Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	877,023	G→T	P338Q (CCG→CAG)	rimO ←	ribosomal protein S12 methylthiotransferase RimO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	877,023	0	G	T	100.0%	92.2 / NA	27	P338Q (CCG→CAG)	rimO	ribosomal protein S12 methylthiotransferase RimO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/15);  total (12/15)

ATTTCACGGCCCACTTTCTCTTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCGGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGTTGAGCGTAGGGTCAGTTCCGGGCAGATTT  >  NC_000913/876888‑877158                                                                                                                                        |                                                                                                                                        aTTTCACGGCCCACTTTCTCTTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCt                                                                                                                                     <  2:232082/140‑1 (MQ=255)       cGGCCCACTTTCTCTTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTAttt                                                                                                                               >  1:219408/1‑140 (MQ=255)          cccACTTTCTCTTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTaaa                                                                                                                            >  2:59982/1‑140 (MQ=255)            cACTTTCTCTTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAagc                                                                                                                          >  1:4965/1‑140 (MQ=255)                    ctTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAAcg                                                                                                                  >  2:41155/1‑140 (MQ=255)                    ctTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAAcg                                                                                                                  <  1:74919/140‑1 (MQ=255)                    ctTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAAcg                                                                                                                  <  1:74820/140‑1 (MQ=255)                      tGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAAcgcg                                                                                                                <  2:219408/140‑1 (MQ=255)                             gcTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGa                                                                                                         <  1:235903/140‑1 (MQ=255)                                cggcggAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGAcgc                                                                                                      >  2:196085/1‑140 (MQ=255)                                   cggAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGct                                                                                                   <  2:106823/140‑1 (MQ=255)                                            gctgctgcAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAgg                                                                                          <  1:61131/140‑1 (MQ=255)                                            gctgctgcAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAgg                                                                                          <  2:4965/140‑1 (MQ=255)                                                                cGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGaa                                                                      >  1:160535/1‑140 (MQ=255)                                                                   ttCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTgg                                                                        <  1:135713/135‑1 (MQ=255)                                                                   ttCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTgg                                                                        >  2:135713/1‑135 (MQ=255)                                                                            tcttctTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGt                                                          <  1:41155/140‑1 (MQ=255)                                                                             cttcttTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGtc                                                         >  1:136878/1‑140 (MQ=255)                                                                                   tAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCa                                                   <  2:160535/140‑1 (MQ=255)                                                                                            ccGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCACCAGGGAAGCCg                                          <  1:223234/140‑1 (MQ=255)                                                                                                        caggcaggGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGtt                              <  1:196085/140‑1 (MQ=255)                                                                                                         aggcaggGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGTTg                             <  2:136878/140‑1 (MQ=255)                                                                                                          ggcaggGCATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGTTGa                            >  1:283529/1‑140 (MQ=255)                                                                                                                 cATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGTTg                             <  1:265596/132‑1 (MQ=255)                                                                                                                 cATTGGCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGTTg                             >  2:265596/1‑132 (MQ=255)                                                                                                                     ggCGTCTGCACCTTCAACCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGTTGAGCGTAGGGTCa                 >  1:241148/1‑140 (MQ=255)                                                                                                                                    aaCCTGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGTGAAGCCGACAATAAAGGTTGAGCGTAGGGTCAGTTCCGGGCAGAttt  >  1:300382/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTTCACGGCCCACTTTCTCTTGCAGGCGCTCGGCGGAAATCTGCTGCTGCAGCTGCATGAAACGGTTCCAGCGTTCTTCTTTAACTTCTTCCGGAACCTGGTCAGGCAGGGCATTGGCGTCTGCACCTTCAACCGGGCTGTATTTAAAGCAGCCAACGCGATCCAGACGCGCTTCTTTCAGGAAGTCGAGTAGCATCTGGAAATCTTCTTCTGTCTCGCCAGGGAAGCCGACAATAAAGGTTGAGCGTAGGGTCAGTTCCGGGCAGATTT  >  NC_000913/876888‑877158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑