Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,974,054	C→A	G424V (GGG→GTG)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,974,054	0	C	A	100.0%	87.4 / NA	27	G424V (GGG→GTG)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/16);  total (11/16)

GGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCCCCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTGGCAGTTCAATACCGTGATCGAGG  >  NC_000913/1973918‑1974182                                                                                                                                         |                                                                                                                                 ggAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAgcaccg                                                                                                                               <  2:688327/140‑1 (MQ=255)     aCGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAgcaccgcccc                                                                                                                           <  2:139229/140‑1 (MQ=255)             gcccTGCTCTGCCGTGGAGAAGCCAGGTGAAAATATCAGCGTCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAGCCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCGCGGTTTAGCACCGCCCCATCGTCgg                                                                                                                   <  2:303776/138‑1 (MQ=255)              aCCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGt                                                                                                                  <  1:318207/140‑1 (MQ=255)              aCCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGt                                                                                                                  <  1:708362/140‑1 (MQ=255)                        ccGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATg                                                                                                        >  2:609157/1‑140 (MQ=255)                             gagaAGCCAGGTTCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAAt                                                                                                   >  2:302819/1‑140 (MQ=255)                             gagaAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAAt                                                                                                         >  2:86941/1‑134 (MQ=255)                             gagaAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAAt                                                                                                         <  1:86941/134‑1 (MQ=255)                                aaGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATG‑TTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTg                                                                                               <  1:708848/140‑1 (MQ=255)                                  gCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATggttc                                                                                              >  2:177348/1‑136 (MQ=255)                                  gCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTgc                                                                                              <  2:375401/140‑1 (MQ=255)                                  gCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTgc                                                                                              >  2:212504/1‑140 (MQ=255)                                  gCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTgc                                                                                              >  2:141498/1‑140 (MQ=255)                                           aaaTATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCCCGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGAt                                                                                     <  2:104160/140‑1 (MQ=255)                                           aaaTATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGAt                                                                                     <  1:212504/140‑1 (MQ=255)                                                       cgcgACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGcagaca                                                                         >  2:421609/1‑140 (MQ=255)                                                         cgACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGcagacaga                                                                       >  1:467397/1‑140 (MQ=255)                                                                        tCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACg                                                        <  1:141498/140‑1 (MQ=255)                                                                        tCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACg                                                        <  1:609157/140‑1 (MQ=255)                                                                        tCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACg                                                        <  1:302819/140‑1 (MQ=255)                                                                        tCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACg                                                        <  2:652804/140‑1 (MQ=255)                                                                               ttCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACg                                                        <  1:674796/133‑1 (MQ=255)                                                                               ttCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACg                                                        >  2:674796/1‑133 (MQ=255)                                                                                                        gCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTgg                        <  1:421609/140‑1 (MQ=255)                                                                                                        gCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTgg                        >  1:361777/1‑140 (MQ=255)                                                                                                        gCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTgg                        <  1:29785/140‑1 (MQ=255)                                                                                                          cGCTTTTGCCAGAATTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTGGCa                      >  1:50229/1‑140 (MQ=255)                                                                                                                       aTTCGCTCACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTGGCAGTTCAATACCGTg         <  1:792771/140‑1 (MQ=255)                                                                                                                              cACGGTTTAGCACCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTGGCAGTTCAATACCGTGATCGAgg  <  2:361777/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 GGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTCGTCGTCGCTCATGTTTTCGCTGACAGTCAAACCTTGCGAGGCCGCTTTTGCCAGAATTCGCTCACGGTTTAGCCCCGCCCCATCGTCGGTCACTTCAATGCAAATGTTGCCGCCCTGATGTTCGGCAGACAGAATTAAATTTCCGACGCTGTTTTTACCTGCGGCGAGCCGTTTTTCTGGCAGTTCAATACCGTGATCGAGG  >  NC_000913/1973918‑1974182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑