Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,537,862	C→A	F38L (TTC→TTA)	yfeS →	conserved protein YfeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,537,862	0	C	A	100.0%	163.8 / NA	47	F38L (TTC→TTA)	yfeS	conserved protein YfeS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/23);  total (24/23)

GGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCTACTATTTTGATGATGAAGA  >  NC_000913/2537740‑2537996                                                                                                                           |                                                                                                                                       ggaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGa                                                                                                                       <  1:699031/140‑1 (MQ=255) ggaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGa                                                                                                                       <  1:442742/140‑1 (MQ=255)  gaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGa                                                                                                                       <  2:144950/139‑1 (MQ=255)  gaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGa                                                                                                                       >  1:144950/1‑139 (MQ=255)  gaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGa                                                                                                                       >  1:681775/1‑139 (MQ=255)  gaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGa                                                                                                                       <  2:681775/139‑1 (MQ=255)     gTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAAt                                                                                                                   >  1:202083/1‑140 (MQ=255)     gTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAAt                                                                                                                   >  2:26151/1‑140 (MQ=255)      tAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATg                                                                                                                  <  2:202083/140‑1 (MQ=255)           tGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATgaagaa                                                                                                             <  1:780065/140‑1 (MQ=255)           tGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATgaagaa                                                                                                             <  1:679789/140‑1 (MQ=255)           tGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATgaagaa                                                                                                             <  1:252664/140‑1 (MQ=255)             aaaaaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACa                                                                                                           <  1:26151/140‑1 (MQ=255)               aaaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAgt                                                                                                         >  2:644544/1‑140 (MQ=255)                      tttatttaTCACGATGAAAAATCGAATAAATTTTGGGGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGCAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGaa                                                                                                  >  1:41374/1‑140 (MQ=255)                       ttatttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGaaa                                                                                                 <  1:644544/140‑1 (MQ=255)                            tatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGc                                                                                            <  1:623617/140‑1 (MQ=255)                               cACGATGAAAAATCGAATAAATTTTGGAGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAg                                                                                         >  1:265729/1‑140 (MQ=255)                                  gATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaaga                                                                                               >  1:460686/1‑131 (MQ=255)                                  gATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaaga                                                                                               <  2:460686/131‑1 (MQ=255)                                    tGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAat                                                                                    >  2:504271/1‑140 (MQ=255)                                    tGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAat                                                                                    >  1:726238/1‑140 (MQ=255)                                    tGAAAAATCGAATAAATTTTGGCGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAat                                                                                    >  2:332445/1‑140 (MQ=255)                                              aaTAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGc                                                                          >  1:647921/1‑140 (MQ=255)                                                           ggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGCCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGaaa                                                             <  1:623750/140‑1 (MQ=255)                                                                      gAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCaaga                                                  >  2:773048/1‑140 (MQ=255)                                                                      gAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCaaga                                                  <  1:504271/140‑1 (MQ=255)                                                                        aGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCaagaag                                                >  1:448010/1‑140 (MQ=255)                                                                          gggATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGAt                                              >  2:154878/1‑140 (MQ=255)                                                                           ggATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATc                                             >  2:198686/1‑140 (MQ=255)                                                                           ggATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATc                                             >  1:549135/1‑140 (MQ=255)                                                                             aTAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCa                                           >  2:400579/1‑140 (MQ=255)                                                                                  ttAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGtt                                      <  1:773048/140‑1 (MQ=255)                                                                                   tAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGttt                                     <  2:265729/140‑1 (MQ=255)                                                                                      cTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATc                                             <  1:385918/129‑1 (MQ=255)                                                                                      cTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATc                                             >  2:385918/1‑129 (MQ=255)                                                                                         tCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTc                               >  2:231446/1‑140 (MQ=255)                                                                                           aaCTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCAt                             >  2:190995/1‑140 (MQ=255)                                                                                               aTGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGAt                         <  2:448010/140‑1 (MQ=255)                                                                                                tGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGttt                                     <  2:347793/127‑1 (MQ=255)                                                                                                tGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGttt                                     >  1:347793/1‑127 (MQ=255)                                                                                                  gCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGc                      <  1:764430/140‑1 (MQ=255)                                                                                                              gtaTTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCTACTATTTtgat          <  2:41374/140‑1 (MQ=255)                                                                                                              gtaTTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCTACTATTTtgat          <  2:647921/140‑1 (MQ=255)                                                                                                                 ttGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCTACTATTTtgatgat       >  2:614537/1‑140 (MQ=255)                                                                                                                   ggTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCTACTATTTtgatgatga     <  1:231446/140‑1 (MQ=255)                                                                                                                      aaaTTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCTACTATTTTGATGATgaaga  >  2:619654/1‑140 (MQ=255)                                                                                                                           |                                                                                                                                       GGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGCTACTATTTTGATGATGAAGA  >  NC_000913/2537740‑2537996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑