Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,618,576	C→A	L67F (TTG→TTT)	hda ←	inibitor of reinitiation of DNA replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,576	0	C	A	100.0%	168.3 / NA	47	L67F (TTG→TTT)	hda	inibitor of reinitiation of DNA replication
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/23);  total (24/23)

AACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAGAGGAG  >  NC_000913/2618442‑2618696                                                                                                                                       |                                                                                                                         aaCTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTc                                                                                                                     <  1:595122/140‑1 (MQ=255) aaCTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTc                                                                                                                     >  2:251642/1‑140 (MQ=255)                aTACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTg                                                                                                      <  1:726483/140‑1 (MQ=255)                aTACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTg                                                                                                      <  1:644169/140‑1 (MQ=255)                     cTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTgcagca                                                                                                 >  1:412145/1‑140 (MQ=255)                     cTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTgcagca                                                                                                 <  2:185766/140‑1 (MQ=255)                         aTGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATg                                                                                             >  2:341712/1‑140 (MQ=255)                         aTGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATg                                                                                             >  2:535038/1‑140 (MQ=255)                         aTGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATg                                                                                             >  1:587501/1‑140 (MQ=255)                             tgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGTGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTg                                                                                         >  1:339863/1‑140 (MQ=255)                             tgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTg                                                                                         >  1:384076/1‑140 (MQ=255)                             tgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTg                                                                                         <  2:176792/140‑1 (MQ=255)                               tCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTgcg                                                                                       >  2:550920/1‑140 (MQ=255)                               tCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTgcg                                                                                       >  2:590589/1‑140 (MQ=255)                                  aTACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGccc                                                                                    >  2:145877/1‑140 (MQ=255)                                        aCCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGcc                                                                              >  2:438234/1‑140 (MQ=255)                                        aCCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGcc                                                                              <  1:251642/140‑1 (MQ=255)                                        aCCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGcc                                                                              <  2:587501/140‑1 (MQ=255)                                             cGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGTGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTcgc                                                                         >  2:473470/1‑140 (MQ=255)                                              gACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGTGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTcgcg                                                                        <  2:339863/140‑1 (MQ=255)                                              gACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTcgcg                                                                        <  1:648707/140‑1 (MQ=255)                                                  aaTGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGcc                                                                    <  1:721800/140‑1 (MQ=255)                                                   aTGCTCCATACCGTCGAGCACTTCCGGGACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGccc                                                                   <  1:11773/140‑1 (MQ=255)                                                   aTGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGccc                                                                   <  1:590589/140‑1 (MQ=255)                                                   aTGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGccc                                                                   <  1:438234/140‑1 (MQ=255)                                                    tGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCa                                                                  <  1:341712/140‑1 (MQ=255)                                                           tACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGagatag                                                           >  2:103671/1‑140 (MQ=255)                                                                tCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGcc                                                                    >  1:51223/1‑126 (MQ=255)                                                                tCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGcc                                                                    <  1:120755/126‑1 (MQ=255)                                                                tCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGcc                                                                    <  2:51223/126‑1 (MQ=255)                                                                tCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGcc                                                                    >  2:120755/1‑126 (MQ=255)                                                                                      aGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACgcagc                                <  1:103671/140‑1 (MQ=255)                                                                                                  ccAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAgc                    >  2:463767/1‑140 (MQ=255)                                                                                                     gCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGcgcg                 >  1:652305/1‑140 (MQ=255)                                                                                                     gCGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGcgcg                 >  1:18150/1‑140 (MQ=255)                                                                                                      cGGGACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCgg                >  1:680381/1‑140 (MQ=255)                                                                                                       gggACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAgcgc                  <  2:226985/137‑1 (MQ=255)                                                                                                       gggACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAgcgc                  >  1:226985/1‑137 (MQ=255)                                                                                                       gggACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGc               <  1:535038/140‑1 (MQ=255)                                                                                                       gggACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGc               <  1:550920/140‑1 (MQ=255)                                                                                                       gggACATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGc               <  2:384076/140‑1 (MQ=255)                                                                                                           cATAGCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGt           >  2:193643/1‑140 (MQ=255)                                                                                                               gCCCACCGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAag       <  2:412145/140‑1 (MQ=255)                                                                                                                    ccGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAgaggag  >  1:133132/1‑140 (MQ=255)                                                                                                                    ccGCATCGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAgaggag  >  2:194750/1‑140 (MQ=255)                                                                                                                          cGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCa             >  1:142894/1‑123 (MQ=255)                                                                                                                          cGCCACGCTGCGAAAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCa             <  2:142894/123‑1 (MQ=255)                                                                                                                                       |                                                                                                                         AACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAGAGGAG  >  NC_000913/2618442‑2618696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑