Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,737,242	G→T	W30L (TGG→TTG)	raiA →	stationary phase translation inhibitor and ribosome stability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,737,242	0	G	T	100.0%	255.0 / NA	73	W30L (TGG→TTG)	raiA	stationary phase translation inhibitor and ribosome stability factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/39);  total (34/39)

GGAACGGTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATGGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGATATGTACACCGCAATTAA  >  NC_000913/2737105‑2737377                                                                                                                                          |                                                                                                                                        ggAACGGTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGc                                                                                                                                       >  2:268858/1‑140 (MQ=255)  gAACGGTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCa                                                                                                                                      <  2:72737/140‑1 (MQ=255)    aCGGTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCaaa                                                                                                                                    <  1:785074/140‑1 (MQ=255)    aCGGTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCaaa                                                                                                                                    <  2:227914/140‑1 (MQ=255)    aCGGTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCaaa                                                                                                                                    <  2:393345/140‑1 (MQ=255)       gTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAacac                                                                                                                                 >  1:470663/1‑140 (MQ=255)       gTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGACATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAacac                                                                                                                                 >  2:764896/1‑140 (MQ=255)        tATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAAcaca                                                                                                                                >  2:572762/1‑140 (MQ=255)         aTGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACAt                                                                                                                               <  2:457482/140‑1 (MQ=255)         aTGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACAt                                                                                                                               <  1:322747/140‑1 (MQ=255)          tGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATc                                                                                                                              >  1:304121/1‑140 (MQ=255)           gCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCt                                                                                                                             >  2:154253/1‑140 (MQ=255)             tGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGa                                                                                                                           >  2:182878/1‑140 (MQ=255)               aaTTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCaaa                                                                                                                                    <  2:506941/129‑1 (MQ=255)               aaTTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCaaa                                                                                                                                    >  1:506941/1‑129 (MQ=255)               aaTTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGAtt                                                                                                                         <  2:304121/140‑1 (MQ=255)               aaTTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGAtt                                                                                                                         >  2:779645/1‑140 (MQ=255)                aTTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTa                                                                                                                        <  2:549100/140‑1 (MQ=255)                 ttCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTaa                                                                                                                       >  2:498051/1‑140 (MQ=255)                   cACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATc                                                                                                                     <  2:198561/140‑1 (MQ=255)                    aCCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATcc                                                                                                                    >  2:166882/1‑140 (MQ=255)                    aCCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATcc                                                                                                                    <  1:154253/140‑1 (MQ=255)                    aCCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATcc                                                                                                                    <  1:166882/140‑1 (MQ=255)                         gACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACata                                                                                                               <  1:748072/140‑1 (MQ=255)                          aCGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACatat                                                                                                              <  1:779645/140‑1 (MQ=255)                              gAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCAtt                                                                                                          >  2:456423/1‑140 (MQ=255)                                                 tATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAg                                                                                       >  2:542602/1‑140 (MQ=255)                                                    gACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGt                                                                                    <  2:40601/140‑1 (MQ=255)                                                        aTGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTttgt                                                                                >  1:619325/1‑140 (MQ=255)                                                            aCATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCt                                                                            <  2:523365/140‑1 (MQ=255)                                                                tACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACg                                                                        >  2:286806/1‑140 (MQ=255)                                                                 aCCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGc                                                                       >  1:299986/1‑140 (MQ=255)                                                                    aGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCcac                                                                    <  2:256212/140‑1 (MQ=255)                                                                    aGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCcac                                                                    <  1:271245/140‑1 (MQ=255)                                                                     gCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCcaca                                                                   >  2:308760/1‑140 (MQ=255)                                                                     gCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCcaca                                                                   >  1:660377/1‑140 (MQ=255)                                                                     gCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCcaca                                                                   >  1:103879/1‑140 (MQ=255)                                                                     gCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATAAATCCACATATCATTCTGTCTAAAGAGCCACAAGGGTTTGTTGCTGACGCcaca                                                                   <  2:470663/140‑1 (MQ=255)                                                                       aaacaaaTGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCAcaat                                                                 >  2:304413/1‑140 (MQ=255)                                                                            aaTGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATa                                                            <  1:182878/140‑1 (MQ=255)                                                                                    ttACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAAc                                                    >  2:193847/1‑140 (MQ=255)                                                                                     tACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAGATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACg                                                   >  1:175520/1‑140 (MQ=255)                                                                                     tACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACg                                                   >  2:673956/1‑140 (MQ=255)                                                                                     tACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACg                                                   >  2:554792/1‑140 (MQ=255)                                                                                        tCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCg                                                <  2:103879/140‑1 (MQ=255)                                                                                           ggCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGG‑AAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCt                                            >  2:33263/1‑140 (MQ=255)                                                                                            gCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCAcaatcaat                                                             >  2:443680/1‑123 (MQ=255)                                                                                            gCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCAcaatcaat                                                             <  1:443680/123‑1 (MQ=255)                                                                                            gCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCt                                            >  2:320325/1‑140 (MQ=255)                                                                                                 ccGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTg                                       >  2:155418/1‑140 (MQ=255)                                                                                                  cGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGc                                      <  1:764896/140‑1 (MQ=255)                                                                                                   gCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGcc                                     <  1:304413/140‑1 (MQ=255)                                                                                                         aTGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGt                               <  2:619325/140‑1 (MQ=255)                                                                                                         aTGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGt                               <  1:498051/140‑1 (MQ=255)                                                                                                          tGTCGCAGACCGTCTCGCCAAACTGGAAAGATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTa                              <  2:175520/140‑1 (MQ=255)                                                                                                          tGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTa                              <  1:268858/140‑1 (MQ=255)                                                                                                          tGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTa                              <  1:286806/140‑1 (MQ=255)                                                                                                          tGTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTa                              <  2:645758/140‑1 (MQ=255)                                                                                                           gTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTaa                             <  2:299986/140‑1 (MQ=255)                                                                                                           gTCGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTaa                             >  2:498952/1‑140 (MQ=255)                                                                                                             cGCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAAc                           <  1:456423/140‑1 (MQ=255)                                                                                                              gCAGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACa                          <  1:554792/140‑1 (MQ=255)                                                                                                                aGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATg                        <  1:572762/140‑1 (MQ=255)                                                                                                                aGACCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATg                        <  1:498952/140‑1 (MQ=255)                                                                                                                  aCCGTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGaa                      <  2:660377/140‑1 (MQ=255)                                                                                                                    cGTCTCGCCAAACTGG‑AAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGat                   <  1:33263/140‑1 (MQ=255)                                                                                                                     gTCTCGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGat                   <  1:193847/140‑1 (MQ=255)                                                                                                                      tctcGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGata                  >  2:289853/1‑140 (MQ=255)                                                                                                                      tctcGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGata                  >  2:76598/1‑140 (MQ=255)                                                                                                                      tctcGCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCA‑‑‑‑CAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGATATGTa              >  2:652599/1‑140 (MQ=255)                                                                                                                          gCCAAACTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGATATGTa              <  1:155418/140‑1 (MQ=255)                                                                                                                              aaCTGGAAAAATTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGATATGTACAcc          >  1:138586/1‑140 (MQ=255)                                                                                                                                      aaaTTGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGATATGTACACCGCAATTaa  >  1:209314/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GGAACGGTATGCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTTATGACAATGAACATTACCAGCAAACAAATGGAAATTACTCCGGCCATCCGCCAACATGTCGCAGACCGTCTCGCCAAACTGGAAAAATGGCAAACACATCTGATTAATCCACATATCATTCTGTCCAAAGAGCCACAAGGGTTTGTTGCTGACGCCACAATCAATACACCTAACGGCGTTCTGGTTGCCAGTGGTAAACATGAAGATATGTACACCGCAATTAA  >  NC_000913/2737105‑2737377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑