Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,896,127	G→T	G143G (GGC→GGA)	ygcR ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,896,127	0	G	T	100.0%	109.3 / NA	32	G143G (GGC→GGA)	ygcR	putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/15);  total (17/15)

TTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAG  >  NC_000913/2895992‑2896262                                                                                                                                        |                                                                                                                                        ttCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGccatccca                                                                                                                                     >  1:433344/1‑140 (MQ=255)                         cACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCg                                                                                                             <  1:15870/140‑1 (MQ=255)                             gCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTg                                                                                                         >  1:777905/1‑140 (MQ=255)                              cAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGc                                                                                                        <  2:777905/140‑1 (MQ=255)                              cAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGc                                                                                                        <  1:291259/140‑1 (MQ=255)                                    aGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTc                                                                                                  >  2:554933/1‑140 (MQ=255)                                              gCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCgc                                                                                        <  1:737204/140‑1 (MQ=255)                                                        aaCCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCa                                                                              <  1:613068/140‑1 (MQ=255)                                                              tGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGt                                                                        <  1:219683/140‑1 (MQ=255)                                                                ttCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTaa                                                                      <  1:520746/140‑1 (MQ=255)                                                                     tACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATc                                                                 >  2:115909/1‑140 (MQ=255)                                                                       cGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAg                                                               <  1:591351/140‑1 (MQ=255)                                                                         cTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGAt                                                             <  1:115909/140‑1 (MQ=255)                                                                          tGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATc                                                            >  1:726432/1‑140 (MQ=255)                                                                            ttCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCa                                                          >  2:546931/1‑140 (MQ=255)                                                                            ttCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCa                                                          >  1:176340/1‑140 (MQ=255)                                                                             tCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAg                                                         >  1:276456/1‑140 (MQ=255)                                                                             tCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAg                                                         <  2:135499/140‑1 (MQ=255)                                                                                    tGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTc                                                  >  1:423000/1‑140 (MQ=255)                                                                                      aTAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTg                                                >  1:641683/1‑140 (MQ=255)                                                                                      aTAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTg                                                >  1:320370/1‑140 (MQ=255)                                                                                          aCAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGAtgc                                            <  2:276456/140‑1 (MQ=255)                                                                                             gcgcGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGAtgctgc                                         >  2:535282/1‑140 (MQ=255)                                                                                              cgcgTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGcc                                        >  1:365866/1‑140 (MQ=255)                                                                                               gcgTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCa                                       <  1:546931/140‑1 (MQ=255)                                                                                                cgTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCAt                                      >  1:600979/1‑140 (MQ=255)                                                                                                               gCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGTCAATATGgcga                       <  2:320370/140‑1 (MQ=255)                                                                                                               gCTCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGgcga                       >  2:171326/1‑140 (MQ=255)                                                                                                                 tCCACCTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGgcgagc                     >  2:709062/1‑140 (MQ=255)                                                                                                                      cTGGGTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACaa                >  1:91602/1‑140 (MQ=255)                                                                                                                        gggTGAAGCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAAt              >  2:211543/1‑140 (MQ=255)                                                                                                                               gCAGGGCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGgcg       <  2:365866/140‑1 (MQ=255)                                                                                                                                    gCCATCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAg  <  2:91602/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAG  >  NC_000913/2895992‑2896262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑