Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,539	G→T	E271D (GAG→GAT)	yghU →	disulfide reductase/organic hydroperoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,539	0	G	T	100.0%	124.1 / NA	35	E271D (GAG→GAT)	yghU	disulfide reductase/organic hydroperoxide reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/18);  total (17/18)

TGCCGCTGAGTTTCTTGATGCGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCT  >  NC_000913/3139404‑3139671                                                                                                                                        |                                                                                                                                     tgCCGCTGAGTTTCTTGATGCGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGcc                                                                                                                                  >  2:635208/1‑140 (MQ=255)   ccGCTGAGTTTCTTGATGCGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCAt                                                                                                                                <  1:163384/140‑1 (MQ=255)      cTGAGTTTCTTGATGCGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGAc                                                                                                                             >  2:190936/1‑140 (MQ=255)             tCTTGATGCGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTg                                                                                                                      >  1:397793/1‑140 (MQ=255)                    gCGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCga                                                                                                               >  1:485748/1‑140 (MQ=255)                     cGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCgag                                                                                                              >  1:453086/1‑140 (MQ=255)                       ggCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGAc                                                                                                            >  2:699216/1‑140 (MQ=255)                        gCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACg                                                                                                           <  1:16971/140‑1 (MQ=255)                            ttATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATa                                                                                                       <  1:635208/140‑1 (MQ=255)                              ataAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACg                                                                                                     <  2:397793/140‑1 (MQ=255)                              ataAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACg                                                                                                     <  2:322318/140‑1 (MQ=255)                              ataAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACg                                                                                                     <  2:485748/140‑1 (MQ=255)                                aaGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGa                                                                                                   <  2:696757/140‑1 (MQ=255)                                     tGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATa                                                                                              <  1:699216/140‑1 (MQ=255)                                     tGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATa                                                                                              <  1:698326/140‑1 (MQ=255)                                      gTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATaa                                                                                             >  2:419346/1‑140 (MQ=255)                                      gTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATaa                                                                                             >  1:244202/1‑140 (MQ=255)                                       tACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCCCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAg                                                                                            >  1:311105/1‑140 (MQ=255)                                            cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCa                                                                                       <  1:419346/140‑1 (MQ=255)                                                 ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGt                                                                                  <  1:728906/140‑1 (MQ=255)                                                 ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGt                                                                                  >  1:237647/1‑140 (MQ=255)                                                 ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGt                                                                                  <  2:237647/140‑1 (MQ=255)                                                 ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGt                                                                                  <  2:453086/140‑1 (MQ=255)                                                 ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGt                                                                                  <  1:319376/140‑1 (MQ=255)                                                 ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGt                                                                                  >  2:488999/1‑140 (MQ=255)                                                                          gcggTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAg                                                         <  1:190936/140‑1 (MQ=255)                                                                                                  aaCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTc                                 >  1:177658/1‑140 (MQ=255)                                                                                                       cACCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgcc                            >  2:458991/1‑140 (MQ=255)                                                                                                        aCCAACGGACCGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccg                           >  1:509007/1‑140 (MQ=255)                                                                                                               gACCGCTGAATGAGCAGTTGCATGATCGCAATGGCGCCAGTGATTTCGAGACGAACACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTCTTCGTCCCCGCAAGCCAGCCGGGCCGGATAGGGCTTTCCCGCCGCATCCgg                    <  2:564835/140‑1 (MQ=255)                                                                                                                  cGCTGAATGAGCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                 <  2:69387/140‑1 (MQ=255)                                                                                                                  cGCTGAATGAGCAGTTGCATGATCCCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                 <  2:311105/140‑1 (MQ=255)                                                                                                                            gCAGTTGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGG‑AATCGTGCACAAt      >  2:301019/1‑140 (MQ=255)                                                                                                                                 tGCATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGGTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCt  >  1:574871/1‑140 (MQ=255)                                                                                                                                   cATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca        <  2:401821/132‑1 (MQ=255)                                                                                                                                   cATGATCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca        >  1:401821/1‑132 (MQ=255)                                                                                                                                        |                                                                                                                                     TGCCGCTGAGTTTCTTGATGCGGGCAGTTATAAGCATGTACAACGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCT  >  NC_000913/3139404‑3139671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑