Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,192,579	C→A	P124P (CCC→CCA)	yqiJ →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,192,579	0	C	A	100.0%	171.0 / NA	48	P124P (CCC→CCA)	yqiJ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/31);  total (17/31)

TGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCCTGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACA  >  NC_000913/3192444‑3192713                                                                                                                                        |                                                                                                                                       tGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGa                                                                                                                                    <  2:768113/140‑1 (MQ=255)    tCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTc                                                                                                                                 <  1:473239/140‑1 (MQ=255)      ttATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCa                                                                                                                               >  1:100566/1‑140 (MQ=255)       tATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCAc                                                                                                                              <  1:768171/140‑1 (MQ=255)       tATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCAc                                                                                                                              <  1:90818/140‑1 (MQ=255)         ttGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCAcgc                                                                                                                            <  2:317905/140‑1 (MQ=255)            gCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACAATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGAc                                                                                                                         <  2:381068/140‑1 (MQ=255)             cATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGAcc                                                                                                                        >  1:478235/1‑140 (MQ=255)              aTCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCa                                                                                                                       >  1:197324/1‑140 (MQ=255)                ccTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATa                                                                                                                     >  2:735364/1‑140 (MQ=255)                  tGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGt                                                                                                                   <  2:612240/140‑1 (MQ=255)                  tGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGt                                                                                                                   >  1:625743/1‑140 (MQ=255)                  tGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGt                                                                                                                   <  1:625472/140‑1 (MQ=255)                    ttGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTc                                                                                                                 <  2:625743/140‑1 (MQ=255)                       cagcaTGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGc                                                                                                              <  2:197324/140‑1 (MQ=255)                       cagcaTGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGc                                                                                                              <  2:511004/140‑1 (MQ=255)                               cTGCATCATGGTCTGGCAATCACCACTGTCTAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACag                                                                                                      <  2:12612/140‑1 (MQ=255)                               cTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACag                                                                                                      <  2:278758/140‑1 (MQ=255)                                 gCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACagaa                                                                                                    <  2:478235/140‑1 (MQ=255)                                 gCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACagaa                                                                                                    <  1:448574/140‑1 (MQ=255)                                 gCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACagaa                                                                                                    <  2:597076/140‑1 (MQ=255)                                  catcatGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACagaag                                                                                                   <  2:791588/140‑1 (MQ=255)                                   atcatGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACagaaga                                                                                                  <  2:444423/140‑1 (MQ=255)                                    tcatGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACagaagaa                                                                                                 <  2:196967/140‑1 (MQ=255)                                     catGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACagaagaag                                                                                                <  2:100566/140‑1 (MQ=255)                                       tGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAgaagaagaa                                                                                              >  2:679276/1‑140 (MQ=255)                                                      cacTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCAGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCGGCTATTACAGAAGAAGAat                                                                                             >  2:248948/1‑126 (MQ=255)                                                      cacTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCAGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCGGCTATTACAGAAGAAGAat                                                                                             <  1:248948/126‑1 (MQ=255)                                                        cTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCa                                                                                 >  1:501406/1‑136 (MQ=255)                                                        cTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCa                                                                                 <  2:501406/136‑1 (MQ=255)                                                         tGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCg                                                                            <  2:650586/140‑1 (MQ=255)                                                              aaCCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCAt                                                                                >  2:141937/1‑131 (MQ=255)                                                              aaCCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCAt                                                                                <  1:141937/131‑1 (MQ=255)                                                                ccTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTa                                                                     >  2:719330/1‑140 (MQ=255)                                                                        tGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGtcat                                                             <  1:679276/140‑1 (MQ=255)                                                                                     tttACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCAc                                                     >  2:34080/1‑135 (MQ=255)                                                                                     tttACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCAc                                                     <  1:34080/135‑1 (MQ=255)                                                                                               aCGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTg                                      >  2:506035/1‑140 (MQ=255)                                                                                                           gTGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACt                          <  1:735364/140‑1 (MQ=255)                                                                                                            tGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGaaa                             <  1:119041/136‑1 (MQ=255)                                                                                                            tGCATTATACCGGTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGaaa                             >  2:119041/1‑136 (MQ=255)                                                                                                                        gTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTATCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAAttt             >  1:282900/1‑140 (MQ=255)                                                                                                                        gTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAAttt             >  1:288670/1‑140 (MQ=255)                                                                                                                        gTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAAttt             >  2:608605/1‑140 (MQ=255)                                                                                                                        gTAAAATAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAAttt             >  2:122468/1‑140 (MQ=255)                                                                                                                        gTAAAATAGTTGCGCCATGGATTCCACGCAACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAAttt             >  2:558461/1‑140 (MQ=255)                                                                                                                            aagaGTTGCGCCATGGATTCCACGCGATGATAGTTCAGCTATATCAGAAAAAAAAAATATTGGTAGCATGGGGTTAATTACCGGTCATCAAGCCACTTCAGTTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGcc         <  2:282900/137‑1 (MQ=255)                                                                                                                            aaTAGTTGCGCCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGcc         <  1:122468/140‑1 (MQ=255)                                                                                                                                   gcgcCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACa  <  2:514650/140‑1 (MQ=255)                                                                                                                                   gcgcCATGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACa  <  1:608605/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TGGTCTTATTGGCATCCTGTTGCAGCATGCCTGCATCATGGTCTGGCAATCACCACTGTCGAACCTGTTCGTGGTTCCTGTCAGTTTACTGTTTACGATTATTGCGGTGCATTATACCGGTAAAATAGTTGCGCCCTGGATTCCACGCGACCATAGTTCAGCTATTACAGAAGAAGAATATATTGGTAGCATGGCGTTAATTACCGGTCATCAAGCCACTTCAGGTAATCCCTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACA  >  NC_000913/3192444‑3192713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑