| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,127,940 | C→A | intergenic (‑46/+138) | yiiX ← / ← metJ | putative lipid binding hydrolase/DNA‑binding transcriptional repressor MetJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,127,940 | 0 | C | A | 95.9% | 168.3 / ‑2.0 | 49 | intergenic (‑46/+138) | yiiX/metJ | putative lipid binding hydrolase/DNA‑binding transcriptional repressor MetJ |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (21/26); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
TGAAAGATGATGTCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCG > NC_000913/4127809‑4128062 | tGAAAGATGATGTCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCgttgtt > 1:630999/1‑140 (MQ=255) tgatgTCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCg > 2:604707/1‑140 (MQ=255) tgatgTCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCg > 2:403736/1‑140 (MQ=255) atgTCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGa < 1:155995/140‑1 (MQ=255) atgTCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAACAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGa > 2:24511/1‑140 (MQ=255) tCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATgc < 1:397761/140‑1 (MQ=255) tCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATgc > 2:54866/1‑140 (MQ=255) cGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATgcg < 2:769011/140‑1 (MQ=255) gCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATgcgg > 2:164833/1‑140 (MQ=255) gTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTg < 2:363711/140‑1 (MQ=255) gTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTg < 1:391001/140‑1 (MQ=255) ggCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGcc < 2:674781/140‑1 (MQ=255) ccAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATc < 1:4498/140‑1 (MQ=255) ggTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGtt < 1:164833/140‑1 (MQ=255) aaaCCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCg > 1:684055/1‑122 (MQ=255) aaaCCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCg < 2:684055/122‑1 (MQ=255) agcagGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCa > 2:252559/1‑140 (MQ=255) agcagGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCa > 1:38765/1‑140 (MQ=255) cagGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAAt > 2:790662/1‑140 (MQ=255) cagGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAAt < 2:630999/140‑1 (MQ=255) cagGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAAt < 1:54866/140‑1 (MQ=255) agGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATa < 1:125321/140‑1 (MQ=255) gCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATaaa < 1:370912/140‑1 (MQ=255) gCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATaaa < 1:403736/140‑1 (MQ=255) tGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAAtt > 1:420757/1‑140 (MQ=255) aGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGc > 1:144531/1‑140 (MQ=255) agcagcCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATg > 2:715010/1‑140 (MQ=255) agcCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACg < 1:604707/140‑1 (MQ=255) agcCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACg < 1:252559/140‑1 (MQ=255) ccTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTa < 1:715010/140‑1 (MQ=255) tGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAgg < 1:24511/140‑1 (MQ=255) tGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAgg > 1:251475/1‑140 (MQ=255) tGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAgg < 2:38765/140‑1 (MQ=255) gTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGc < 2:420757/140‑1 (MQ=255) ttAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCt < 1:3784/131‑1 (MQ=255) ttAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCt > 2:3784/1‑131 (MQ=255) ttAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGt > 1:242479/1‑140 (MQ=255) ttAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGt > 1:123303/1‑140 (MQ=255) tCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCAt < 2:144531/140‑1 (MQ=255) tCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCAt < 1:790662/140‑1 (MQ=255) cATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCa > 2:619511/1‑140 (MQ=255) cATGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCa > 1:421280/1‑140 (MQ=255) aTGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAg > 1:621865/1‑140 (MQ=255) tGTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAgg < 2:151400/140‑1 (MQ=255) gTATAAAAAGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGc < 2:123303/140‑1 (MQ=255) aaaaGCGGTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTc > 1:215078/1‑140 (MQ=255) cGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTc > 1:329376/1‑140 (MQ=255) gTGGGTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCcg < 2:421280/140‑1 (MQ=255) gggTCGCAGACAACGTGATCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCcgcg < 2:370443/140‑1 (MQ=255) | TGAAAGATGATGTCGCCGGTTTGTGGCTGCCAGGCAAAGGCAGGTACAGAAACCAGCAGGCTGAGGATCAGCAGCCTGTTTTTCATAGTTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCG > NC_000913/4127809‑4128062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |