Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,383,471	C→T	H277Y (CAC→TAC)	epmA →	EF‑P‑lysine lysyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,383,471	0	C	T	100.0%	173.9 / NA	49	H277Y (CAC→TAC)	epmA	EF‑P‑lysine lysyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/22);  total (27/22)

TCGCTGAACGCTTTGAGGTTTATTATAAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGCACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTGAAGTCAT  >  NC_000913/4383337‑4383595                                                                                                                                       |                                                                                                                             tCGCTGAACGCTTTGAGGTTTATTATAAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTAcccc                                                                                                                         >  2:562727/1‑140 (MQ=255)   gCTGAACGCTTTGAGGTTTATTATAAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCAt                                                                                                                       <  1:539864/140‑1 (MQ=255)          gCTTTGAGGTTTATTATAAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAg                                                                                                                <  1:756613/140‑1 (MQ=255)                      attatAAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGTACAAGATAACCGTAAGCGCGCGGCGCGCGGTATGCCGCAGCACCCCATTGACCAGAATCTGATTCaa                                                                                                    >  2:651610/1‑140 (MQ=255)                             aGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGaa                                                                                                    <  1:374461/133‑1 (MQ=255)                             aGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGaa                                                                                                    >  2:374461/1‑133 (MQ=255)                                 aTTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGt                                                                                         >  2:16162/1‑140 (MQ=255)                                 aTTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGt                                                                                         >  1:665916/1‑140 (MQ=255)                                 aTTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGAAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGt                                                                                         >  2:167959/1‑140 (MQ=255)                                  ttGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTc                                                                                        >  2:434498/1‑140 (MQ=255)                                  ttGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTc                                                                                        >  2:506833/1‑140 (MQ=255)                                  ttGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTc                                                                                        >  2:707187/1‑140 (MQ=255)                                    gAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCgg                                                                                      >  1:61740/1‑140 (MQ=255)                                     aGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGt                                                                                     <  1:52307/140‑1 (MQ=255)                                      gCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTa                                                                                    <  2:471398/140‑1 (MQ=255)                                       cTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCt                                                                                                <  1:395461/127‑1 (MQ=255)                                       cTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCt                                                                                                >  2:395461/1‑127 (MQ=255)                                         ggCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGc                                                                                 >  1:393827/1‑140 (MQ=255)                                          gCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGcc                                                                                >  2:314842/1‑140 (MQ=255)                                             aaTGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGa                                                                             <  1:506833/140‑1 (MQ=255)                                               tGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACt                                                                           >  2:386607/1‑140 (MQ=255)                                                    tCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTcc                                                                      >  2:597047/1‑140 (MQ=255)                                                    tCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTcc                                                                      >  2:680689/1‑140 (MQ=255)                                                    tCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTcc                                                                      >  2:165379/1‑140 (MQ=255)                                                       aTGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGc                                                                   <  2:89964/140‑1 (MQ=255)                                                        tGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCg                                                                  >  2:289789/1‑140 (MQ=255)                                                         gAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGt                                                                 <  1:224463/140‑1 (MQ=255)                                                            ttGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGc                                                                   <  1:124819/135‑1 (MQ=255)                                                            ttGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGc                                                                   >  2:124819/1‑135 (MQ=255)                                                              gACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTTGGTATGCCTGACTGTTCCGGCGTGGCAt                                                            <  2:455122/140‑1 (MQ=255)                                                              gACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCAt                                                            <  2:665916/140‑1 (MQ=255)                                                              gACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCAt                                                            <  1:289789/140‑1 (MQ=255)                                                              gACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCAt                                                            <  1:314842/140‑1 (MQ=255)                                                              gACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCAt                                                            <  1:386607/140‑1 (MQ=255)                                                                      cccGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCAt                                                            >  1:461072/1‑132 (MQ=255)                                                                      cccGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCAt                                                            <  2:461072/132‑1 (MQ=255)                                                                           gAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCg                                               <  1:562727/140‑1 (MQ=255)                                                                                 caacaaCGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGt                                         <  1:167959/140‑1 (MQ=255)                                                                                 caacaaCGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGt                                         >  2:201703/1‑140 (MQ=255)                                                                                 caacaaCGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGt                                         >  1:37346/1‑140 (MQ=255)                                                                                           ttGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGgcg                               >  1:513947/1‑140 (MQ=255)                                                                                           ttGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGgcg                               >  2:410694/1‑140 (MQ=255)                                                                                             gAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCt                             <  1:651610/140‑1 (MQ=255)                                                                                              aaCAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTg                            <  1:707187/140‑1 (MQ=255)                                                                                                   gATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGgcgc                       >  2:354349/1‑140 (MQ=255)                                                                                                              gcgcgcggcgcgcgGTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTgg            >  1:739810/1‑140 (MQ=255)                                                                                                                 cgcggcgcgcggTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTg         <  2:393827/140‑1 (MQ=255)                                                                                                                 cgcggcgcgcggTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTg         >  2:18495/1‑140 (MQ=255)                                                                                                                 cgcggcgcgcggTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTg         >  1:747929/1‑140 (MQ=255)                                                                                                                   cggcgcgcggTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTGaa       >  1:778675/1‑140 (MQ=255)                                                                                                                        cgcggTTTGCCGCAGTACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTGAAGTCAt  <  2:513947/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                             TCGCTGAACGCTTTGAGGTTTATTATAAAGGTATTGAGCTGGCGAATGGTTTCCATGAATTGACGGATGCCCGTGAGCAGCAACAACGCTTTGAACAAGATAACCGTAAGCGCGCGGCGCGCGGTTTGCCGCAGCACCCCATTGACCAGAATCTGATTGAAGCCTTGAAAGTCGGTATGCCTGACTGTTCCGGCGTGGCATTAGGTGTTGATCGTCTGGTGATGTTGGCGCTGGGCGCGGAGACACTGGCTGAAGTCAT  >  NC_000913/4383337‑4383595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑