Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,245	G→T	S317I (AGC→ATC)	appB →	cytochrome bd‑II ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,245	0	G	T	100.0%	116.3 / NA	34	S317I (AGC→ATC)	appB	cytochrome bd‑II ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/20);  total (14/20)

TCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTAC  >  NC_000913/1040110‑1040369                                                                                                                                        |                                                                                                                             tcttctGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTg                                                                                                                          <  2:306821/140‑1 (MQ=255)  cttctGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGa                                                                                                                         <  1:585764/140‑1 (MQ=255)       gCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCg                                                                                                                    >  2:87434/1‑140 (MQ=255)             tGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTg                                                                                                                          >  2:82160/1‑128 (MQ=255)             tGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTg                                                                                                                          <  1:82160/128‑1 (MQ=255)                    ggTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGa                                                                                                       <  1:11103/140‑1 (MQ=255)                               tttATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGAc                                                                                            <  1:204159/140‑1 (MQ=255)                               tttATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGAc                                                                                            <  1:453020/140‑1 (MQ=255)                                  aTCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGt                                                                                         >  1:222318/1‑140 (MQ=255)                                             ccGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCa                                                                              >  1:25648/1‑140 (MQ=255)                                                ggTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCt                                                                           >  1:265418/1‑140 (MQ=255)                                                      ggATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGTAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCt                                                                     <  2:265418/140‑1 (MQ=255)                                                      ggATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCt                                                                     <  2:25648/140‑1 (MQ=255)                                                           tttGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCa                                                                <  1:711783/140‑1 (MQ=255)                                                             tGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCAtt                                                              <  2:102675/140‑1 (MQ=255)                                                               aTGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTAt                                                            <  2:222318/140‑1 (MQ=255)                                                                 ggCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGt                                                          >  2:744831/1‑140 (MQ=255)                                                                    atcatTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTgg                                                       >  1:71837/1‑140 (MQ=255)                                                                           acgCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGt                                                <  2:658915/138‑1 (MQ=255)                                                                                aTTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGa                                           >  1:771334/1‑140 (MQ=255)                                                                                aTTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGa                                           <  1:287383/140‑1 (MQ=255)                                                                                   tGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGatat                                        >  2:94795/1‑140 (MQ=255)                                                                                   tGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGatat                                        >  1:655137/1‑140 (MQ=255)                                                                                       gTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATAttttt                                    <  2:263928/140‑1 (MQ=255)                                                                                       gTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATAttttt                                    <  1:744831/140‑1 (MQ=255)                                                                                           ttttCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGccc                                >  2:752953/1‑140 (MQ=255)                                                                                            tttCACGGGAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTTCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCa                               <  2:207520/140‑1 (MQ=255)                                                                                                           cACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTaca                <  1:631229/140‑1 (MQ=255)                                                                                                           cACGCTGTTCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTaca                <  1:191319/140‑1 (MQ=255)                                                                                                                  ttCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTaca                <  2:463364/133‑1 (MQ=255)                                                                                                                  ttCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTaca                >  1:463364/1‑133 (MQ=255)                                                                                                                  ttCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTg         >  2:482370/1‑140 (MQ=255)                                                                                                                  ttCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTg         >  1:570090/1‑140 (MQ=255)                                                                                                                   tCCCCTTTGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTgg        <  2:655137/140‑1 (MQ=255)                                                                                                                         ttGTCATGCCGTCAATCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGctac  <  1:94795/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                             TCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTAC  >  NC_000913/1040110‑1040369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑