| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,139,960 | C→A | A528E (GCG→GAG) | flgK → | flagellar hook‑filament junction protein 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,139,960 | 0 | C | A | 94.9% | 121.5 / 1.2 | 39 | A528E (GCG→GAG) | flgK | flagellar hook‑filament junction protein 1 |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (15/22); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
CCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACC > NC_000913/1139825‑1140083 | ccGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAAt > 1:389322/1‑140 (MQ=255) cgcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATg > 1:784982/1‑140 (MQ=255) gcgACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATgc > 2:273832/1‑140 (MQ=255) gACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATgcgc < 2:784982/140‑1 (MQ=255) gACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATgcgc < 1:720751/140‑1 (MQ=255) aCGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCa > 1:180942/1‑140 (MQ=255) gTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAgg < 1:680595/140‑1 (MQ=255) gTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACg > 1:590630/1‑140 (MQ=255) gTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACg > 2:732606/1‑140 (MQ=255) tAGCGCCACGCAAGGTAATGTGGTGACGCANCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACgg > 2:541038/1‑140 (MQ=255) aGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGCCGGc < 2:589627/140‑1 (MQ=255) aCGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGCCGGCAAAcgc > 2:706026/1‑140 (MQ=255) tgtgGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATg < 1:541038/140‑1 (MQ=255) gtggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgc < 2:590630/140‑1 (MQ=255) tggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcg < 1:273832/140‑1 (MQ=255) tggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcg < 2:389322/140‑1 (MQ=255) tggtgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATgcg < 2:180942/140‑1 (MQ=255) tgACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTg > 2:516862/1‑140 (MQ=255) aCGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAt > 2:777783/1‑140 (MQ=255) cGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGAtt < 2:509639/140‑1 (MQ=255) cTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACa > 2:611062/1‑139 (MQ=255) cTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACa < 1:611062/139‑1 (MQ=255) cTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACAt < 2:260182/140‑1 (MQ=255) tCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAg < 1:516862/140‑1 (MQ=255) cagcagTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGga > 2:421632/1‑140 (MQ=255) agTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATa > 1:13947/1‑140 (MQ=255) agTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATa < 1:628699/140‑1 (MQ=255) tttCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGc > 1:619924/1‑140 (MQ=255) ttCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCg < 2:383419/140‑1 (MQ=255) cGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGttt < 1:85377/140‑1 (MQ=255) cGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGttt < 1:777783/140‑1 (MQ=255) cGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGttt < 1:519604/140‑1 (MQ=255) tgtCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAg < 1:706026/140‑1 (MQ=255) aggagTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGt < 2:13947/140‑1 (MQ=255) gTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCa > 2:392816/1‑140 (MQ=255) aCGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGc > 1:730913/1‑140 (MQ=255) cGTTTTCAGCAGTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGt < 1:732606/140‑1 (MQ=255) cagcagTATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCAcc < 1:392816/140‑1 (MQ=255) tATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCAcc < 2:475816/134‑1 (MQ=255) tATTACCTGGAGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCAcc > 1:475816/1‑134 (MQ=255) | CCGCGACGTTGAAAACCAGTAGCGCCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGATTTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAGTATTACCTGGCGAATGCGCAGGTTCTGCAGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAAGGGGAGATAAGATGCGTTTCAGTACACAGATGATGTACCAGCAAAACATGCGTGGTATCACC > NC_000913/1139825‑1140083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |