Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,107	C→A	W206C (TGG→TGT)	ydhW ←	protein YdhW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,107	0	C	A	100.0%	184.5 / NA	54	W206C (TGG→TGT)	ydhW	protein YdhW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/28);  total (26/28)

TTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGC  >  NC_000913/1750974‑1751238                                                                                                                                      |                                                                                                                                    ttCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCg                                                                                                                               >  2:669976/1‑140 (MQ=255)   cccATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGca                                                                                                                             <  2:711113/140‑1 (MQ=255)    ccATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcat                                                                                                                            <  1:314569/140‑1 (MQ=255)     cATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcata                                                                                                                           <  1:88627/140‑1 (MQ=255)     cATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcata                                                                                                                           <  2:698313/140‑1 (MQ=255)           cAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcataagccat                                                                                                                     >  1:297190/1‑140 (MQ=255)             gAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcataagccataa                                                                                                                   >  2:381167/1‑140 (MQ=255)               aCAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcataagccataagc                                                                                                                 <  2:432243/140‑1 (MQ=255)               aCAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcataagccataagc                                                                                                                 <  1:606441/140‑1 (MQ=255)               aCAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGcataagccataagc                                                                                                                 <  2:267319/140‑1 (MQ=255)                   aTTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTg                                                                                                             >  2:146972/1‑140 (MQ=255)                   aTTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTg                                                                                                             >  1:672916/1‑140 (MQ=255)                       gCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCAt                                                                                                         <  2:233593/140‑1 (MQ=255)                        cGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATa                                                                                                        >  2:333875/1‑140 (MQ=255)                        cGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATa                                                                                                        >  2:439537/1‑140 (MQ=255)                              gTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCAt                                                                                                  <  1:333875/140‑1 (MQ=255)                                    gACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcg                                                                                            <  2:364476/140‑1 (MQ=255)                                       aTCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctc                                                                                         >  2:399342/1‑140 (MQ=255)                                        tCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcg                                                                                        <  1:626590/140‑1 (MQ=255)                                        tCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAAtcgctcg                                                                                        >  1:57662/1‑140 (MQ=255)                                            aTTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTaa                                                                                    <  1:399342/140‑1 (MQ=255)                                              tATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAAc                                                                                  <  1:669976/140‑1 (MQ=255)                                              tATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAAc                                                                                  <  1:449967/140‑1 (MQ=255)                                               aTCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACa                                                                                 >  2:303257/1‑140 (MQ=255)                                                 ccccTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAg                                                                               <  2:57662/140‑1 (MQ=255)                                                   ccTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTa                                                                             >  1:167987/1‑140 (MQ=255)                                                        tGCAGTGGCTGCAACTGTCCCCCCTACCCATGACATCCCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTacaaca                                                                        <  2:212721/140‑1 (MQ=255)                                                          cAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACAcc                                                                      >  1:50144/1‑140 (MQ=255)                                                            gTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATTAATCGCTCGCTAAACAAGTACAACAccgc                                                                    >  1:112855/1‑140 (MQ=255)                                                                 tGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCg                                                               <  2:297349/140‑1 (MQ=255)                                                                         tCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGa                                                           >  2:606901/1‑136 (MQ=255)                                                                         tCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGa                                                           <  1:606901/136‑1 (MQ=255)                                                                         tCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTcc                                                       >  2:558256/1‑140 (MQ=255)                                                                           cACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATTAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCAc                                                     <  2:112855/140‑1 (MQ=255)                                                                           cACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCAc                                                     <  1:558256/140‑1 (MQ=255)                                                                           cACCCTACCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCAc                                                     <  2:753635/140‑1 (MQ=255)                                                                                 aTCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCg                                               <  2:297190/140‑1 (MQ=255)                                                                                   ccATGACATCTCCCTGAAATTTCAGGGGTTCTGAGACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGta                                             >  2:565837/1‑140 (MQ=255)                                                                                     aTGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGtata                                           <  2:672916/140‑1 (MQ=255)                                                                                     aTGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGtata                                           <  1:146972/140‑1 (MQ=255)                                                                                     aTGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGtata                                           <  1:381167/140‑1 (MQ=255)                                                                                        aCATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCg                                        <  1:689988/140‑1 (MQ=255)                                                                                            ctcCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGtata                                           >  2:440351/1‑133 (MQ=255)                                                                                            ctcCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGtata                                           <  1:440351/133‑1 (MQ=255)                                                                                            ctcCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCat                                    >  2:5553/1‑140 (MQ=255)                                                                                            ctcCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCat                                    >  1:496515/1‑140 (MQ=255)                                                                                            ctcCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCat                                    >  1:307343/1‑140 (MQ=255)                                                                                            ctcCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCat                                    >  2:736542/1‑140 (MQ=255)                                                                                                cTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTc                                <  2:511276/140‑1 (MQ=255)                                                                                                cTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTc                                <  1:736542/140‑1 (MQ=255)                                                                                                   aaaTTTCAGGGGTTCTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAgg                             >  2:533219/1‑140 (MQ=255)                                                                                                                 cTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGt                            <  2:187141/127‑1 (MQ=255)                                                                                                                 cTGAAACTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGt                            >  1:187141/1‑127 (MQ=255)                                                                                                                       cTGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGa         >  1:287131/1‑140 (MQ=255)                                                                                                                        tGCTCTACCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGAt        >  2:647824/1‑140 (MQ=255)                                                                                                                              aCCTCGAAACATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGc  >  1:530254/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGC  >  NC_000913/1750974‑1751238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑