Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,512,937	G→T	intergenic (‑231/‑105)	mntH ← / → nupC	Mn(2(+))/Fe(2(+)): H(+) symporter MntH/nucleoside:H(+) symporter NupC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,512,937	0	G	T	100.0%	332.1 / NA	93	intergenic (‑231/‑105)	mntH/nupC	Mn(2(+))/Fe(2(+)): H(+) symporter MntH/nucleoside:H(+) symporter NupC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/54);  total (39/54)

TGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCA  >  NC_000913/2512801‑2513071                                                                                                                                         |                                                                                                                                       tGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCt                                                                                                                                     <  1:818893/140‑1 (MQ=255)      gACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTcac                                                                                                                                >  2:658263/1‑140 (MQ=255)       aCCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTcaca                                                                                                                               >  1:786897/1‑140 (MQ=255)       aCCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTcaca                                                                                                                               >  1:84506/1‑140 (MQ=255)       aCCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTcaca                                                                                                                               >  2:336058/1‑140 (MQ=255)       aCCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTcaca                                                                                                                               >  1:96724/1‑140 (MQ=255)         cAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTT‑ATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            >  1:620816/1‑140 (MQ=255)         cAGGATTATGACTACGAAAAGATT‑ATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:826539/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACGCAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:479606/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:925273/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:85781/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:461573/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:861654/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:433371/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:37339/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:282724/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  2:898746/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  2:855819/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  2:786897/140‑1 (MQ=255)          aGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  2:332476/140‑1 (MQ=255)            gATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACg                                                                                                                          <  2:298016/140‑1 (MQ=255)            gATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACg                                                                                                                          <  2:19277/140‑1 (MQ=255)            gATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACg                                                                                                                          <  1:594566/140‑1 (MQ=255)            gATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACg                                                                                                                          <  1:689092/140‑1 (MQ=255)            gATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACg                                                                                                                          <  1:809291/140‑1 (MQ=255)            gATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACg                                                                                                                          <  2:168887/140‑1 (MQ=255)                 tGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCAtt                                                                                                                     >  1:463136/1‑140 (MQ=255)                 tGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCAtt                                                                                                                     >  1:925568/1‑140 (MQ=255)                 tGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCAtt                                                                                                                     >  2:769213/1‑140 (MQ=255)                  gACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATta                                                                                                                    <  1:889353/140‑1 (MQ=255)                  gACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATta                                                                                                                    <  2:84506/140‑1 (MQ=255)                    cTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            >  2:641834/1‑130 (MQ=255)                    cTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGa                                                                                                                            <  1:641834/130‑1 (MQ=255)                    cTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATtata                                                                                                                  <  2:96724/140‑1 (MQ=255)                      aCGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAgt                                                                                                                <  2:767721/140‑1 (MQ=255)                      aCGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAgt                                                                                                                <  1:392623/140‑1 (MQ=255)                      aCGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAgt                                                                                                                >  2:104939/1‑140 (MQ=255)                                 gatgaCTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGAt                                                                                                     >  1:509176/1‑140 (MQ=255)                                 gatgaCTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGAt                                                                                                     <  2:622904/140‑1 (MQ=255)                                  atgaCTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGAtc                                                                                                    >  2:407183/1‑140 (MQ=255)                                            tGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCt                                                                                          <  1:713424/140‑1 (MQ=255)                                             gttatggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCtt                                                                                         <  1:104939/140‑1 (MQ=255)                                             gttatggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCtt                                                                                         <  1:336058/140‑1 (MQ=255)                                               tatggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTaa                                                                                       >  2:580989/1‑140 (MQ=255)                                               tatggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTaa                                                                                       >  1:108459/1‑140 (MQ=255)                                                 tggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAAcc                                                                                     <  2:137652/140‑1 (MQ=255)                                                 tggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAAcc                                                                                     <  2:250535/140‑1 (MQ=255)                                                 tggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAAcc                                                                                     <  2:789302/140‑1 (MQ=255)                                                 tggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAAcc                                                                                     <  1:72194/140‑1 (MQ=255)                                                 tggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAAcc                                                                                     <  1:407183/140‑1 (MQ=255)                                                   gttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCAt                                                                                   <  1:440776/140‑1 (MQ=255)                                                    ttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATg                                                                                  >  1:145407/1‑140 (MQ=255)                                                     tatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGt                                                                                 <  2:152650/140‑1 (MQ=255)                                                        gTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTAc                                                                              >  2:124300/1‑140 (MQ=255)                                                        gTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTAc                                                                              >  2:53937/1‑140 (MQ=255)                                                         tttAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACa                                                                             >  1:329166/1‑140 (MQ=255)                                                             aTGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGa                                                                         <  1:363531/140‑1 (MQ=255)                                                             aTGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGa                                                                         >  2:293851/1‑140 (MQ=255)                                                             aTGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGa                                                                         <  2:108459/140‑1 (MQ=255)                                                             aTGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGa                                                                         <  2:341854/140‑1 (MQ=255)                                                                 tAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAAtgt                                                                     >  1:194159/1‑140 (MQ=255)                                                                  aGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAAtgtg                                                                    >  2:218887/1‑140 (MQ=255)                                                                  aGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAAtgtg                                                                    >  1:225177/1‑140 (MQ=255)                                                                      caTTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACg                                                                <  2:509176/140‑1 (MQ=255)                                                                        tttACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGa                                                              >  1:841209/1‑140 (MQ=255)                                                                                aCTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAAc                                                      <  2:620816/140‑1 (MQ=255)                                                                                aCTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAAc                                                      <  2:329166/140‑1 (MQ=255)                                                                                           ccATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCatat                                           >  2:9075/1‑140 (MQ=255)                                                                                               acacAACATAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTgg                                       <  2:302992/140‑1 (MQ=255)                                                                                                 acaacaTAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAg                                     <  2:948478/140‑1 (MQ=255)                                                                                                 acaacaTAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAg                                     <  2:278586/140‑1 (MQ=255)                                                                                                     caTAGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa                                 >  1:795733/1‑140 (MQ=255)                                                                                                        aGCAGAAATGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAatat                              >  1:325834/1‑140 (MQ=255)                                                                                                             aaaTGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGAcc                         <  2:925568/140‑1 (MQ=255)                                                                                                               aTGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAg                                     >  1:698444/1‑126 (MQ=255)                                                                                                               aTGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAg                                     <  2:698444/126‑1 (MQ=255)                                                                                                                tGTATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACcgcg                      <  2:220300/140‑1 (MQ=255)                                                                                                                  tATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTc                    >  2:630605/1‑140 (MQ=255)                                                                                                                  tATGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTc                    >  1:876212/1‑140 (MQ=255)                                                                                                                    tGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCt                  <  2:841209/140‑1 (MQ=255)                                                                                                                    tGACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCt                  <  1:297093/140‑1 (MQ=255)                                                                                                                     gACAGATCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCtt                 <  2:145407/140‑1 (MQ=255)                                                                                                                          aTCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAttt            >  1:177092/1‑140 (MQ=255)                                                                                                                          aTCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAttt            >  2:339096/1‑140 (MQ=255)                                                                                                                          aTCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAttt            <  1:769213/140‑1 (MQ=255)                                                                                                                          aTCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAttt            >  1:317533/1‑140 (MQ=255)                                                                                                                          aTCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAttt            >  1:317141/1‑140 (MQ=255)                                                                                                                          aTCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtt             <  2:243592/139‑1 (MQ=255)                                                                                                                          aTCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtt             >  1:243592/1‑139 (MQ=255)                                                                                                                           tCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtttt           >  2:820007/1‑140 (MQ=255)                                                                                                                           tCACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCAtttt           >  2:573109/1‑140 (MQ=255)                                                                                                                            cACTATTTTTGAATCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTg          >  2:647566/1‑140 (MQ=255)                                                                                                                                 tttttGAATCCTGTCACAGGACATCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCG‑‑‑CCTTCATTTTGTACTGGCa  >  1:284794/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCA  >  NC_000913/2512801‑2513071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑