Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,757,829	C→A	E258* (GAA→TAA)	yfjH ←	CP4‑57 prophage; protein YfjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,757,829	0	C	A	100.0%	220.3 / NA	62	E258* (GAA→TAA)	yfjH	CP4‑57 prophage; protein YfjH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/36);  total (26/36)

ACATCTTCATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCAC  >  NC_000913/2757693‑2757963                                                                                                                                         |                                                                                                                                       aCATCTTCATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGAt                                                                                                                                     <  2:521894/140‑1 (MQ=255)  cATCTTCATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATa                                                                                                                                    <  2:49976/140‑1 (MQ=255)  cATCTTCATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATa                                                                                                                                    <  2:488896/140‑1 (MQ=255)    tCTTCATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAg                                                                                                                                  <  2:709519/140‑1 (MQ=255)      ttCATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGtt                                                                                                                                >  2:39741/1‑140 (MQ=255)        cATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCa                                                                                                                              <  1:737133/140‑1 (MQ=255)          ttttttAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCAtt                                                                                                                            <  1:882453/140‑1 (MQ=255)               tAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTg                                                                                                                       >  2:696827/1‑140 (MQ=255)                  aGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTa                                                                                                                    <  2:562767/140‑1 (MQ=255)                                    aGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCAcc                                                                                                  <  2:407729/140‑1 (MQ=255)                                     gTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCAccc                                                                                                 >  1:435726/1‑140 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTATCTGCTACAACCTTATTTTTCCACCca                                                                                                >  1:327484/1‑140 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                <  2:523616/140‑1 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                >  1:856432/1‑140 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                <  2:109488/140‑1 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                <  2:641448/140‑1 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                <  2:694342/140‑1 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                <  2:821617/140‑1 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                <  2:865854/140‑1 (MQ=255)                                      tATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCca                                                                                                <  1:388983/140‑1 (MQ=255)                                       aTCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCcat                                                                                               >  2:369303/1‑140 (MQ=255)                                                aGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCCCCCATCATTCGGaa                                                                                      <  2:218398/140‑1 (MQ=255)                                                aGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGaa                                                                                      >  2:656254/1‑140 (MQ=255)                                                aGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGaa                                                                                      <  1:389727/140‑1 (MQ=255)                                                      ttGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGt                                                                                >  1:691508/1‑140 (MQ=255)                                                                  tgCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCt                                                                    <  1:429027/140‑1 (MQ=255)                                                                      acacGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTcaac                                                                >  1:57433/1‑140 (MQ=255)                                                                       cacGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTcaacc                                                               >  2:748577/1‑140 (MQ=255)                                                                       cacGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTcaacc                                                               <  2:435726/140‑1 (MQ=255)                                                                         cGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTcaaccaa                                                             >  1:842897/1‑140 (MQ=255)                                                                         cGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTcaaccaa                                                             >  1:284839/1‑140 (MQ=255)                                                                            tatTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACtt                                                          <  1:389784/140‑1 (MQ=255)                                                                               tttttATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAg                                                       <  1:432583/140‑1 (MQ=255)                                                                               tttttATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAg                                                       <  2:853381/140‑1 (MQ=255)                                                                               tttttATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAg                                                       <  2:654309/140‑1 (MQ=255)                                                                               tttttATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAg                                                       <  1:656254/140‑1 (MQ=255)                                                                               tttttATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAg                                                       <  2:911116/140‑1 (MQ=255)                                                                                 tttATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGtt                                                     <  2:35974/140‑1 (MQ=255)                                                                                 tttATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGtt                                                     <  1:81394/140‑1 (MQ=255)                                                                                    aTTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGaa                                                  >  2:107214/1‑140 (MQ=255)                                                                                      tttGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATg                                                <  2:842897/140‑1 (MQ=255)                                                                                      tttGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATg                                                >  2:514831/1‑140 (MQ=255)                                                                                        tGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGat                                              >  1:325519/1‑140 (MQ=255)                                                                                         gATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGata                                             <  1:592571/140‑1 (MQ=255)                                                                                          attattTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGatat                                            >  2:485091/1‑140 (MQ=255)                                                                                          attattTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGatat                                            >  1:910726/1‑140 (MQ=255)                                                                                             atttatCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGt                                         <  2:277294/140‑1 (MQ=255)                                                                                              tttatCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTa                                        <  1:858400/140‑1 (MQ=255)                                                                                                    tcAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTc                                  >  2:422758/1‑140 (MQ=255)                                                                                                    tcAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTc                                  >  1:71113/1‑140 (MQ=255)                                                                                                    tcAAACTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTc                                  >  1:29979/1‑140 (MQ=255)                                                                                                        aCTCTGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgct                              >  1:317561/1‑140 (MQ=255)                                                                                                            tGACATTTGAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCgctttgc                          <  1:92974/140‑1 (MQ=255)                                                                                                                    gAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCa                  >  1:127059/1‑140 (MQ=255)                                                                                                                    gAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCa                  <  1:366796/140‑1 (MQ=255)                                                                                                                    gAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCa                  <  1:39741/140‑1 (MQ=255)                                                                                                                    gAATAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCa                  >  2:179143/1‑140 (MQ=255)                                                                                                                       tAAAATCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCAccc               <  2:29979/140‑1 (MQ=255)                                                                                                                          aaTCCCATAGCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCcttc            >  2:20454/1‑140 (MQ=255)                                                                                                                                   gCGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCa   <  2:954683/140‑1 (MQ=255)                                                                                                                                    cGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCAc  >  2:100371/1‑140 (MQ=255)                                                                                                                                    cGGTTAGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCAc  >  1:183719/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACATCTTCATTTTTTAAAGACCATTGATTTAAAATAGTATCAACCAAAGCATCTTGACTCATTGTTGCTACACGATATTTTTTATTTTGATTATTTATCTCAAACTCTGACATTTGAATAAAATCCCATAGCGGTTCGATAAGTTCATTAACTGCTACAACCTTATTTTTCCACCCATCATTCGGAACAGAGTCATTAATTAGCTCAACCAACTTCAGTTGAATGATATGGTACACTTCCGCTTTGCTTTTGCCACCCTTCTTTCCTGCAC  >  NC_000913/2757693‑2757963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑