Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,593,037	C→A	R134L (CGG→CTG)	livF ←	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,593,037	0	C	A	100.0%	90.8 / NA	27	R134L (CGG→CTG)	livF	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/14);  total (13/14)

GAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCCGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGC  >  NC_000913/3592907‑3593159                                                                                                                                   |                                                                                                                           gAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCCCCGCCGGACATGGTGCCCGCCAGCTGAATAc                                                                                                                   <  2:645033/140‑1 (MQ=255)   aaaTTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACgg                                                                                                                 <  1:650223/140‑1 (MQ=255)     aTTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGgcg                                                                                                               <  1:827764/140‑1 (MQ=255)                       ggCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGaaa                                                                                             <  2:122559/140‑1 (MQ=255)                          gcAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAg                                                                                          >  2:71121/1‑140 (MQ=255)                          gcAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAg                                                                                          <  1:43576/140‑1 (MQ=255)                              gACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCa                                                                                      <  2:269854/140‑1 (MQ=255)                                   aGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATacac                                                                                 >  1:463431/1‑140 (MQ=255)                                             catcaAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATg                                                                       <  1:293786/140‑1 (MQ=255)                                              atcaAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATgc                                                                      >  2:872357/1‑140 (MQ=255)                                              atcaAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATgc                                                                      <  1:42349/140‑1 (MQ=255)                                                      gTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTgg                                                              <  2:848461/140‑1 (MQ=255)                                                            aaCGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTgg                                                        >  2:329365/1‑140 (MQ=255)                                                             aCGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGt                                                       >  2:466391/1‑140 (MQ=255)                                                             aCGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGt                                                       >  2:684434/1‑140 (MQ=255)                                                              cgcgGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTc                                                      >  2:850089/1‑140 (MQ=255)                                                                    ttGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTTGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTc                                                <  2:243538/140‑1 (MQ=255)                                                                       cTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACAGCCGGACATGGCGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGc                                             >  2:553796/1‑140 (MQ=255)                                                                                      ccAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCAt                              >  2:461093/1‑140 (MQ=255)                                                                                       cAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATc                             <  2:531130/140‑1 (MQ=255)                                                                                             ccAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAgg                       <  2:352502/140‑1 (MQ=255)                                                                                             ccAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAgg                       <  2:500797/140‑1 (MQ=255)                                                                                               aGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGtt                     <  1:103333/140‑1 (MQ=255)                                                                                               aGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGtt                     <  2:750049/140‑1 (MQ=255)                                                                                                            cACCGCCGGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTc        >  2:478966/1‑140 (MQ=255)                                                                                                               cgccgGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATc     >  1:725809/1‑140 (MQ=255)                                                                                                               cgccgGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATc     >  1:262111/1‑140 (MQ=255)                                                                                                                  cgGACATGGTGCCCGCCAGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCcgc  >  1:137980/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                           GAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGCAAACGCGGGTTGCTCATCAGCGCACGACCAATCGCCAGCATCTGCTGTTCACCGCCGGACATGGTGCCCGCCCGCTGAATACGGCGCTCATGCAGACGTGGAAACAGCTCATACACCCACTTTATGCGCTCCTGGAACTGGTCGCGTTCAGCAAAAAAACCGCCCATCGCCAGGTTCTCTTCCACCGTCATCCGC  >  NC_000913/3592907‑3593159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑