Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,389,630	C→A	G244V (GGC→GTC)	psd ←	phosphatidylserine decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,389,630	0	C	A	100.0%	126.4 / NA	37	G244V (GGC→GTC)	psd	phosphatidylserine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/20);  total (17/20)

TCGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAATGCTGCCAACAATCGTCGCTCC  >  NC_000913/4389498‑4389757                                                                                                                                     |                                                                                                                                tCGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAATGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTcag                                                                                                                          <  1:434017/140‑1 (MQ=255)  cGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTcagc                                                                                                                         >  2:102525/1‑140 (MQ=255)    gTAGATACTGCCAGCGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTcagcag                                                                                                                       <  2:881474/140‑1 (MQ=255)          aCTGCCAGCGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCcac                                                                                                                 <  2:727098/140‑1 (MQ=255)          aCTGCCAGCGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCcac                                                                                                                 >  1:897589/1‑140 (MQ=255)                  cGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACcgt                                                                                                         >  1:864436/1‑140 (MQ=255)                    gCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACcgtcg                                                                                                       >  2:794305/1‑140 (MQ=255)                      tGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGtt                                                                                                     >  1:226445/1‑140 (MQ=255)                                   tGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGc                                                                                        <  2:116182/140‑1 (MQ=255)                                         acagGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTc                                                                                  <  2:855114/140‑1 (MQ=255)                                         acagGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTc                                                                                  >  2:426907/1‑140 (MQ=255)                                              cTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAgcg                                                                             <  2:173533/140‑1 (MQ=255)                                              cTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAgcg                                                                             <  1:590359/140‑1 (MQ=255)                                               tttCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAgcgc                                                                            <  2:887212/140‑1 (MQ=255)                                               tttCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAgcgc                                                                            <  2:566866/140‑1 (MQ=255)                                               tttCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGACAGGCCAGGTCCAgcgc                                                                            <  2:63371/140‑1 (MQ=255)                                                 tCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCtt                                                                          >  2:846940/1‑140 (MQ=255)                                                 tCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCtt                                                                          <  1:442314/140‑1 (MQ=255)                                                   cAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTtga                                                                        >  2:262793/1‑140 (MQ=255)                                                    aGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTtgat                                                                       >  1:757040/1‑140 (MQ=255)                                                           cAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCt                                                                >  2:533525/1‑140 (MQ=255)                                                                aGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTcgcg                                                           >  1:336730/1‑140 (MQ=255)                                                                  aTTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTcgcgcg                                                         <  2:877390/140‑1 (MQ=255)                                                                   ttCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcgg                                                        <  1:217814/140‑1 (MQ=255)                                                                     cACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggcg                                                      <  1:563180/140‑1 (MQ=255)                                                                      aCTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggcgg                                                     >  1:802777/1‑140 (MQ=255)                                                                      aCTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggcgg                                                     >  2:202643/1‑140 (MQ=255)                                                                           aCCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTaa                                                <  1:102525/140‑1 (MQ=255)                                                                                gTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTg                                           <  2:757040/140‑1 (MQ=255)                                                                                gTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTg                                           <  1:640161/140‑1 (MQ=255)                                                                                 tGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTgc                                          >  1:310341/1‑140 (MQ=255)                                                                                           ttGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGAc                                >  1:442126/1‑140 (MQ=255)                                                                                                     tGGAACCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAAtg                      <  2:307012/140‑1 (MQ=255)                                                                                                        aaCCGAGTTTAAAGCGACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAAtgctg                   >  2:551684/1‑140 (MQ=255)                                                                                                                       gACCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTTGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAATGCTGCCAACAATCGTCGCt    >  1:113633/1‑140 (MQ=255)                                                                                                                        aCCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAATGCTGCCAACAATCGTCGCTc   <  2:802777/140‑1 (MQ=255)                                                                                                                        aCCCATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAATGCTGCCAACAATCGTCGCTc   <  2:226445/140‑1 (MQ=255)                                                                                                                         cccATTTCCTGGACTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAATGCTGCCAACAATCGTCGCTcc  >  1:577682/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                TCGGTAGATACTGCCAGCGGCTGGCCAATTTTCGTGACAGACAGGCTTTCCAGTTGCTCAACCAGATTCACTTTACCCGGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACCGTCTCAATGCTGCCAACAATCGTCGCTCC  >  NC_000913/4389498‑4389757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑