Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,457,851	G→T	P5P (CCC→CCA)	yjgA ←	DUF615 domain‑containing protein YjgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,457,851	0	G	T	100.0%	256.6 / NA	72	P5P (CCC→CCA)	yjgA	DUF615 domain‑containing protein YjgA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/47);  total (25/47)

TTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTC  >  NC_000913/4457714‑4457984                                                                                                                                          |                                                                                                                                      ttCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCtgg                                                                                                                                     <  2:403340/140‑1 (MQ=255)   ccccAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGgct                                                                                                                                   <  2:550990/140‑1 (MQ=255)        gATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTa                                                                                                                              <  1:352274/140‑1 (MQ=255)         aTCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAg                                                                                                                             <  2:222941/140‑1 (MQ=255)         aTCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAg                                                                                                                             <  1:826320/140‑1 (MQ=255)         aTCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAg                                                                                                                             <  1:176085/140‑1 (MQ=255)         aTCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAg                                                                                                                             <  1:543563/140‑1 (MQ=255)         aTCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAg                                                                                                                             <  2:701603/140‑1 (MQ=255)          tCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGt                                                                                                                            <  2:493878/140‑1 (MQ=255)          tCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGt                                                                                                                            <  2:523093/140‑1 (MQ=255)            aacaaTTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCa                                                                                                                          >  2:173990/1‑140 (MQ=255)                   tCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAgg                                                                                                                   >  1:566305/1‑140 (MQ=255)                   tCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAgg                                                                                                                   >  1:785235/1‑140 (MQ=255)                     cgcgCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCt                                                                                                                 >  1:255023/1‑140 (MQ=255)                           aaGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTaa                                                                                                           <  1:604572/140‑1 (MQ=255)                                gTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTaaaaaaa                                                                                                      <  1:125064/140‑1 (MQ=255)                                      gCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCt                                                                                                <  1:942038/140‑1 (MQ=255)                                       ctcctcGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTa                                                                                               <  2:127565/140‑1 (MQ=255)                                         cctcGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAAt                                                                                             >  2:140391/1‑140 (MQ=255)                                            cGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGtt                                                                                          >  2:834459/1‑140 (MQ=255)                                            cGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGtt                                                                                          <  1:404010/140‑1 (MQ=255)                                                             tCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCa                                                                         <  2:613924/140‑1 (MQ=255)                                                               aCTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACt                                                                       >  1:147031/1‑140 (MQ=255)                                                               aCTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACt                                                                       >  1:575100/1‑140 (MQ=255)                                                                    tACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGaaaa                                                                  >  2:79400/1‑140 (MQ=255)                                                                     aCTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGaaaaa                                                                 <  1:773799/140‑1 (MQ=255)                                                                     aCTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGaaaaa                                                                 <  1:103690/140‑1 (MQ=255)                                                                        gACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACgg                                                              <  2:771846/140‑1 (MQ=255)                                                                         aCCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGt                                                             >  2:255712/1‑140 (MQ=255)                                                                         aCCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGt                                                             <  2:785235/140‑1 (MQ=255)                                                                         aCCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGt                                                             >  1:353827/1‑140 (MQ=255)                                                                          cccAGATAATTTCATCGTCTTCGTCTTCGATGTCATCTCCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGtt                                                            <  2:697631/140‑1 (MQ=255)                                                                          cccAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGtt                                                            <  1:670832/140‑1 (MQ=255)                                                                          cccAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGtt                                                            <  1:124437/140‑1 (MQ=255)                                                                          cccAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGtt                                                            <  1:349074/140‑1 (MQ=255)                                                                           ccAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGtt                                                            >  1:157011/1‑139 (MQ=255)                                                                           ccAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGtt                                                            <  2:157011/139‑1 (MQ=255)                                                                             aGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGTGCACTGAAAAACGGTtctc                                                         >  1:275320/1‑140 (MQ=255)                                                                              gATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTctct                                                        >  2:500645/1‑140 (MQ=255)                                                                                  aTTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTa                                                    <  2:255023/140‑1 (MQ=255)                                                                                   tttCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAg                                                   >  1:903216/1‑140 (MQ=255)                                                                                   tttCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAg                                                   >  2:637210/1‑140 (MQ=255)                                                                                   tttCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAg                                                   >  2:221312/1‑140 (MQ=255)                                                                                     tCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGAc                                                 >  1:471840/1‑140 (MQ=255)                                                                                       aTCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAGAACGGTTCTCTGTTAGACtt                                               <  1:208279/140‑1 (MQ=255)                                                                                        tcgtcttcgtctTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTc                                              >  2:115655/1‑140 (MQ=255)                                                                                         cgtcttcgtcttCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCa                                             >  1:11408/1‑140 (MQ=255)                                                                                         cgtcttcgtcttCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCa                                             >  2:232484/1‑140 (MQ=255)                                                                                            cttcgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGTGCACTGAAAAACGGTTCTCTGTTAGACTTCagag                                          <  2:275320/140‑1 (MQ=255)                                                                                            cttcgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCagag                                          <  1:618069/140‑1 (MQ=255)                                                                                             ttcgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAgaga                                         <  2:147031/140‑1 (MQ=255)                                                                                               cgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGaaa                                       <  2:796839/140‑1 (MQ=255)                                                                                               cgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGaaa                                       >  1:938428/1‑140 (MQ=255)                                                                                               cgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGaaa                                       <  1:221312/140‑1 (MQ=255)                                                                                               cgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGaaa                                       <  2:353827/140‑1 (MQ=255)                                                                                               cgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGaaa                                       <  1:834459/140‑1 (MQ=255)                                                                                               cgtcttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGaaa                                       <  2:471840/140‑1 (MQ=255)                                                                                                   ttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAActct                                   <  1:835219/140‑1 (MQ=255)                                                                                                   ttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAActct                                   <  1:255712/140‑1 (MQ=255)                                                                                                   ttcgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAActct                                   <  1:267894/140‑1 (MQ=255)                                                                                                     cgATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAActctct                                 <  2:220738/140‑1 (MQ=255)                                                                                                      gATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTa                                <  2:392073/140‑1 (MQ=255)                                                                                                            atcaCCGGGAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTAt                          >  2:515682/1‑140 (MQ=255)                                                                                                                  gggAACGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACt                    >  1:647793/1‑140 (MQ=255)                                                                                                                      aCGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCa                <  2:52640/140‑1 (MQ=255)                                                                                                                      aCGTCGTCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCa                <  1:694021/140‑1 (MQ=255)                                                                                                                       cgtcgtCGAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCaa               <  1:836163/140‑1 (MQ=255)                                                                                                                              gAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGt        <  1:270276/140‑1 (MQ=255)                                                                                                                              gAGCCAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGt        <  1:79400/140‑1 (MQ=255)                                                                                                                                 ccAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAAt     <  1:500645/140‑1 (MQ=255)                                                                                                                                 ccAGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAAt     <  1:232484/140‑1 (MQ=255)                                                                                                                                   aGTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAAtct   >  1:342547/1‑140 (MQ=255)                                                                                                                                    gTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAAtctc  <  2:11408/140‑1 (MQ=255)                                                                                                                                    gTCTTCTGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAAtctc  <  1:173990/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTC  >  NC_000913/4457714‑4457984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑