Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,527,292	G→T	P82P (CCC→CCA)	sgcE ←	KpLE2 phage‑like element; putative epimerase SgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,527,292	0	G	T	100.0%	189.1 / NA	56	P82P (CCC→CCA)	sgcE	KpLE2 phage‑like element; putative epimerase SgcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/28);  total (28/28)

CAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAATGAAGCTG  >  NC_000913/4527156‑4527427                                                                                                                                         |                                                                                                                                        cAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGt                                                                                                                                      <  2:20483/140‑1 (MQ=255)  aGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTc                                                                                                                                     <  1:373928/140‑1 (MQ=255)    aCGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGACCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCCGCATGAACGAAGATCCACGCTGGTCTg                                                                                                                                   >  2:62956/1‑140 (MQ=255)         aGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTc                                                                                                                              >  1:952567/1‑140 (MQ=255)                    ggCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCCCGCTGGTCTGATTTCAGCCAGCGCGt                                                                                                                   >  2:890737/1‑140 (MQ=255)                            tCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTT‑AACCAgc                                                                                                          >  2:19711/1‑140 (MQ=255)                                     ccGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTTCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGc                                                                                                  >  2:398460/1‑140 (MQ=255)                                     ccGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGc                                                                                                  <  2:742770/140‑1 (MQ=255)                                         aTTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCggg                                                                                              <  1:359880/140‑1 (MQ=255)                                         aTTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCggg                                                                                              <  1:404287/140‑1 (MQ=255)                                                aCCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCat                                                                                       <  1:191789/140‑1 (MQ=255)                                                aCCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCat                                                                                       <  2:174881/140‑1 (MQ=255)                                                  cAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatga                                                                                     <  2:617476/140‑1 (MQ=255)                                                  cAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatga                                                                                     >  1:578845/1‑140 (MQ=255)                                                   aGTCCGGCTCGTGCGCTGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTTTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaa                                                                                    >  2:591112/1‑140 (MQ=255)                                                   aGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaa                                                                                    >  2:482621/1‑140 (MQ=255)                                                   aGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaa                                                                                    >  1:107183/1‑140 (MQ=255)                                                   aGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaa                                                                                    >  1:932625/1‑140 (MQ=255)                                                   aGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaa                                                                                    >  1:130228/1‑140 (MQ=255)                                                    gTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaa                                                                                   >  2:80423/1‑140 (MQ=255)                                                    gTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaa                                                                                   >  1:940764/1‑140 (MQ=255)                                                    gTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaa                                                                                   >  2:589743/1‑140 (MQ=255)                                                     tCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaat                                                                                  >  1:860587/1‑140 (MQ=255)                                                     tCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaat                                                                                  >  1:647068/1‑140 (MQ=255)                                                     tCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTGTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaat                                                                                  <  2:338883/140‑1 (MQ=255)                                                       cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatga                                                                                <  1:909438/140‑1 (MQ=255)                                                       cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatga                                                                                >  2:657521/1‑140 (MQ=255)                                                        ggCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatgaa                                                                               <  1:782064/140‑1 (MQ=255)                                                           tCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAgg                                                                            <  2:585093/140‑1 (MQ=255)                                                             gTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGAc                                                                          <  2:674514/140‑1 (MQ=255)                                                             gTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGAc                                                                          <  1:922216/140‑1 (MQ=255)                                                                               tctcAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCgtctgt                                                        >  1:31306/1‑140 (MQ=255)                                                                                ctcAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCgtctgtc                                                       >  2:570508/1‑140 (MQ=255)                                                                                        aCGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACg                                               >  2:577036/1‑140 (MQ=255)                                                                                             tCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTg                                          >  1:936680/1‑140 (MQ=255)                                                                                              ccGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCATTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGc                                         >  2:373822/1‑140 (MQ=255)                                                                                                gAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCAc                                       <  2:952567/140‑1 (MQ=255)                                                                                                    gATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGtt                                   <  1:767811/140‑1 (MQ=255)                                                                                                      tAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGtttt                                 >  1:103594/1‑140 (MQ=255)                                                                                                           cAGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATcc                            <  1:577036/140‑1 (MQ=255)                                                                                                            aGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATccc                           <  2:824856/140‑1 (MQ=255)                                                                                                            aGCGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTCAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATccc                           <  1:570197/140‑1 (MQ=255)                                                                                                              cGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCaa                         >  2:929763/1‑140 (MQ=255)                                                                                                              cGTTTCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCaa                         >  2:911197/1‑140 (MQ=255)                                                                                                                 ttCTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACg                      <  2:647068/140‑1 (MQ=255)                                                                                                                   cTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTa                    <  2:534336/140‑1 (MQ=255)                                                                                                                   cTGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTa                    >  2:202418/1‑140 (MQ=255)                                                                                                                    tGCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTT‑AACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAt                  <  1:62956/140‑1 (MQ=255)                                                                                                                     gCATGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAt                  <  1:802572/140‑1 (MQ=255)                                                                                                                       aTGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtgt                <  1:911197/140‑1 (MQ=255)                                                                                                                        tGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtgtt               >  1:148114/1‑140 (MQ=255)                                                                                                                        tGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtgtt               <  2:889707/140‑1 (MQ=255)                                                                                                                        tGAACGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtgtt               >  2:433868/1‑140 (MQ=255)                                                                                                                          aaCGAAGATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtgttgt             >  1:313976/1‑140 (MQ=255)                                                                                                                                gATCCACGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAATGa       <  2:467830/140‑1 (MQ=255)                                                                                                                                     aCGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtg                 >  1:295750/1‑125 (MQ=255)                                                                                                                                     aCGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtg                 <  2:295750/125‑1 (MQ=255)                                                                                                                                     aCGCTGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAATGAAgctg  <  2:107183/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAATGAAGCTG  >  NC_000913/4527156‑4527427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑