Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,080,904	G→T	intergenic (+91/‑452)	putP → / → efeU	proline:Na(+) symporter/inactive ferrous iron permease EfeU, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,080,904	0	G	T	100.0%	57.7 / NA	18	intergenic (+91/‑452)	putP/efeU	proline:Na(+) symporter/inactive ferrous iron permease EfeU, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/8);  total (10/8)

CGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCGG  >  NC_000913/1080770‑1081036                                                                                                                                       |                                                                                                                                     cgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAtcgcgc                                                                                                                                 <  1:266086/140‑1 (MQ=255)              ggCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCaa                                                                                                                    >  1:283114/1‑140 (MQ=255)                cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCCTATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATc                                                                                                                  >  2:372814/1‑140 (MQ=255)                         cACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCgccgccgcc                                                                                                         <  2:567252/140‑1 (MQ=255)                                                  ttAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGaa                                                                                <  1:3936/140‑1 (MQ=255)                                                          cggcggTTTTGTTTTGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAg                                                                        >  1:722925/1‑140 (MQ=255)                                                                          cTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGCACGCTTCAGCGATTTTGACGccacc                                                        >  2:291825/1‑140 (MQ=255)                                                                                    gggTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAg                                              <  2:722925/140‑1 (MQ=255)                                                                                      gTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCt                                            <  2:446550/140‑1 (MQ=255)                                                                                       ttGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTg                                           <  2:798086/140‑1 (MQ=255)                                                                                       ttGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTg                                           <  1:55842/140‑1 (MQ=255)                                                                                       ttGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTg                                           <  1:455728/140‑1 (MQ=255)                                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCaa                         >  1:877095/1‑140 (MQ=255)                                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCaa                         >  2:211914/1‑140 (MQ=255)                                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCaa                         >  1:71803/1‑140 (MQ=255)                                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCaa                         >  2:508045/1‑140 (MQ=255)                                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCaa                         >  2:627266/1‑140 (MQ=255)                                                                                                         ccTCGCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCaa                         >  1:286525/1‑140 (MQ=255)                                                                                                             gCCATATAAATTGAATGGTGCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAg                     >  1:638512/1‑140 (MQ=255)                                                                                                                                gCAGGGATCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCgg  <  1:397756/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATGTTCCGGGCAAAAAGTTAGATTTGATAATCGCGG  >  NC_000913/1080770‑1081036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑