Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,841	G→T	R74L (CGG→CTG)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,841	0	G	T	100.0%	123.8 / NA	37	R74L (CGG→CTG)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/22);  total (15/22)

AATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACT  >  NC_000913/1147705‑1147976                                                                                                                                         |                                                                                                                                        aaTTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGcc                                                                                                                                      <  1:878817/140‑1 (MQ=255)   ttCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCtt                                                                                                                                    <  2:354949/140‑1 (MQ=255)        aTTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCaa                                                                                                                               >  1:192425/1‑140 (MQ=255)                     tcttctTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCa                                                                                                                  >  1:818616/1‑140 (MQ=255)                     tcttctTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCa                                                                                                                  >  2:363390/1‑140 (MQ=255)                     tcttctTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCa                                                                                                                  >  2:906400/1‑140 (MQ=255)                        tcttTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTc                                                                                                               >  1:929701/1‑140 (MQ=255)                               gTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAg                                                                                                        >  2:859858/1‑140 (MQ=255)                                          cGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGcgcg                                                                                             <  1:408154/140‑1 (MQ=255)                                          cGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGcgcg                                                                                             <  1:23632/140‑1 (MQ=255)                                          cGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGcgcg                                                                                             <  1:582688/140‑1 (MQ=255)                                           gACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGt                                                                                            <  1:239919/140‑1 (MQ=255)                                            aCGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTg                                                                                           <  1:269881/140‑1 (MQ=255)                                                     cGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAg                                                                                  <  2:833076/140‑1 (MQ=255)                                                     cGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAg                                                                                  <  2:442406/140‑1 (MQ=255)                                                                          aCTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAgcg                                                             <  1:271731/140‑1 (MQ=255)                                                                          aCTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAgcg                                                             >  1:199832/1‑140 (MQ=255)                                                                                 acaacGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCt                                                      >  2:453122/1‑140 (MQ=255)                                                                                    acGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCtgtg                                                   >  1:240999/1‑140 (MQ=255)                                                                                         cgcgTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGt                                              <  1:424757/140‑1 (MQ=255)                                                                                         cgcgTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGt                                              <  1:279325/140‑1 (MQ=255)                                                                                          gcgTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTg                                             <  2:240999/140‑1 (MQ=255)                                                                                            gTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGcc                                           <  1:406628/140‑1 (MQ=255)                                                                                                      tattCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCg                                 <  1:76521/140‑1 (MQ=255)                                                                                                      tattCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCg                                 <  2:192425/140‑1 (MQ=255)                                                                                                         tCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCgggg                              <  1:514755/140‑1 (MQ=255)                                                                                                         tCCTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCgggg                              <  1:212130/140‑1 (MQ=255)                                                                                                           cTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGgcg                            <  1:883254/140‑1 (MQ=255)                                                                                                           cTGCGCAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGgcg                            >  2:586373/1‑140 (MQ=255)                                                                                                              cgcAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTg                         >  1:544768/1‑140 (MQ=255)                                                                                                              cgcAGTCAGTTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTg                         >  1:539032/1‑140 (MQ=255)                                                                                                                   tcagtTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGAAATACCGGGGCGCTGATggg                    >  2:559889/1‑140 (MQ=255)                                                                                                                    cagtTATCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATgggg                   <  2:929701/140‑1 (MQ=255)                                                                                                                         aTCGCCAGTGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGc              <  1:453122/140‑1 (MQ=255)                                                                                                                                 tGATGCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAattat      >  2:16657/1‑140 (MQ=255)                                                                                                                                     gCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACt  <  1:317291/140‑1 (MQ=255)                                                                                                                                     gCCCTGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACt  >  2:923101/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACT  >  NC_000913/1147705‑1147976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑