Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,598,477	T→C	intergenic (‑391/+140)	yneO ← / ← lsrK	AIDA‑I family autotransporter YneO/autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,598,477	0	T	C	100.0%	284.6 / NA	77	intergenic (‑391/+140)	yneO/lsrK	AIDA‑I family autotransporter YneO/autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/37);  total (40/37)

CATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATG  >  NC_000913/1598343‑1598613                                                                                                                                       |                                                                                                                                         catATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCtt                                                                                                                                     <  1:583921/140‑1 (MQ=255)    atTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGaa                                                                                                                                  <  1:810350/140‑1 (MQ=255)      ttAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAata                                                                                                                                <  2:639604/140‑1 (MQ=255)          cAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGt                                                                                                                            >  2:217074/1‑140 (MQ=255)           aaaGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTa                                                                                                                           >  2:61922/1‑140 (MQ=255)           aaaGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTa                                                                                                                           <  1:523479/140‑1 (MQ=255)            aaGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTAc                                                                                                                          <  2:55729/140‑1 (MQ=255)             aGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACt                                                                                                                         <  1:761366/140‑1 (MQ=255)             aGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACt                                                                                                                         >  2:429700/1‑140 (MQ=255)              gAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTg                                                                                                                        >  2:706735/1‑140 (MQ=255)               aaTAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTgg                                                                                                                       >  2:697505/1‑140 (MQ=255)                aTAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGt                                                                                                                      >  2:618084/1‑140 (MQ=255)                   gCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGAt                                                                                                                   <  2:905232/140‑1 (MQ=255)                         aTTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACg                                                                                                             >  1:49702/1‑140 (MQ=255)                         aTTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACg                                                                                                             >  1:861889/1‑140 (MQ=255)                         aTTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACg                                                                                                             >  2:9745/1‑140 (MQ=255)                             cTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGa                                                                                                         >  2:117009/1‑140 (MQ=255)                             cTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGa                                                                                                         <  2:180005/140‑1 (MQ=255)                              tAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGaa                                                                                                        >  2:46399/1‑140 (MQ=255)                                       aaGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGc                                                                                               >  1:935754/1‑140 (MQ=255)                                            tattaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCt                                                                                          <  2:861889/140‑1 (MQ=255)                                            tattaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCt                                                                                          <  1:586367/140‑1 (MQ=255)                                                  aCCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAAc                                                                                    >  2:55697/1‑140 (MQ=255)                                                      cTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGc                                                                                >  2:238886/1‑140 (MQ=255)                                                       ttttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGcc                                                                               <  2:440879/140‑1 (MQ=255)                                                       ttttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGcc                                                                               <  1:238886/140‑1 (MQ=255)                                                            cATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGctc                                                                          <  2:864757/140‑1 (MQ=255)                                                              tCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTa                                                                        >  1:171186/1‑140 (MQ=255)                                                                   tAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaaga                                                                   <  1:429700/140‑1 (MQ=255)                                                                     gtgtCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaagaag                                                                 <  2:274214/140‑1 (MQ=255)                                                                        tCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGa                                                              >  1:44959/1‑140 (MQ=255)                                                                         cTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGaa                                                             <  2:823420/140‑1 (MQ=255)                                                                          tttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAgg                                                                >  1:675747/1‑136 (MQ=255)                                                                          tttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAgg                                                                <  2:675747/136‑1 (MQ=255)                                                                          tttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGaaa                                                            >  2:424271/1‑140 (MQ=255)                                                                          tttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGaaa                                                            <  2:414737/140‑1 (MQ=255)                                                                          tttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGaaa                                                            <  1:10048/140‑1 (MQ=255)                                                                           ttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAAt                                                           >  1:552144/1‑140 (MQ=255)                                                                           ttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAAt                                                           <  1:210366/140‑1 (MQ=255)                                                                           ttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAAt                                                           <  1:1623/140‑1 (MQ=255)                                                                            tAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATa                                                          >  2:540511/1‑140 (MQ=255)                                                                                aaTATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGaa                                                      >  1:842747/1‑140 (MQ=255)                                                                                aaTATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGaa                                                      >  2:134140/1‑140 (MQ=255)                                                                                  tatTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGAACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATa                                                    >  1:806865/1‑140 (MQ=255)                                                                                   atTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATaa                                                   <  1:83217/140‑1 (MQ=255)                                                                                    ttCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAAc                                                  >  1:541269/1‑140 (MQ=255)                                                                                    ttCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAAc                                                  >  1:52797/1‑140 (MQ=255)                                                                                    ttCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAAc                                                  >  2:898726/1‑140 (MQ=255)                                                                                    ttCGCTATAAAATAAGGGTCGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAAc                                                  >  1:283093/1‑140 (MQ=255)                                                                                         tataAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATAc                                             >  2:36734/1‑140 (MQ=255)                                                                                          ataAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACt                                            <  1:134140/140‑1 (MQ=255)                                                                                                        gtgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACta                                                >  1:14292/1‑122 (MQ=255)                                                                                                        gtgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACta                                                <  2:14292/122‑1 (MQ=255)                                                                                                        gtgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGc                              >  1:153438/1‑140 (MQ=255)                                                                                                        gtgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGc                              >  1:199388/1‑140 (MQ=255)                                                                                                        gtgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGc                              >  2:892507/1‑140 (MQ=255)                                                                                                         tgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGcc                             >  2:46169/1‑140 (MQ=255)                                                                                                         tgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGcc                             >  2:189032/1‑140 (MQ=255)                                                                                                         tgCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGcc                             >  1:914178/1‑140 (MQ=255)                                                                                                          gCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCa                            <  2:388913/140‑1 (MQ=255)                                                                                                          gCTTAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCa                            <  2:261368/140‑1 (MQ=255)                                                                                                            ttAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACg                          <  1:706735/140‑1 (MQ=255)                                                                                                            ttAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACg                          <  1:25385/140‑1 (MQ=255)                                                                                                             tAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACgg                         <  2:240269/140‑1 (MQ=255)                                                                                                             tAATGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACgg                         <  1:337070/140‑1 (MQ=255)                                                                                                              aaTGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGt                        >  2:369434/1‑140 (MQ=255)                                                                                                              aaTGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGt                        >  2:611999/1‑140 (MQ=255)                                                                                                                tGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCAc                           >  2:70523/1‑135 (MQ=255)                                                                                                                tGCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCAc                           <  1:70523/135‑1 (MQ=255)                                                                                                                 gCTTTAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcat                     <  1:406914/140‑1 (MQ=255)                                                                                                                   tttAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATGACTATACTCAAAAACTAACAGCCACGGtcatca                   <  2:935754/140‑1 (MQ=255)                                                                                                                   tttAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcatca                   >  1:491843/1‑140 (MQ=255)                                                                                                                   tttAAGAAAAATAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcatca                   >  2:140056/1‑140 (MQ=255)                                                                                                                           aaaTAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTgg           <  1:419204/140‑1 (MQ=255)                                                                                                                             aTAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCt         <  1:424271/140‑1 (MQ=255)                                                                                                                             aTAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCt         <  1:611999/140‑1 (MQ=255)                                                                                                                             aTAGCAATTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCt         <  2:199388/140‑1 (MQ=255)                                                                                                                                   aTTTCCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAAt   <  2:842747/140‑1 (MQ=255)                                                                                                                                    tttcCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATg  <  2:658331/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATG  >  NC_000913/1598343‑1598613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑