Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,835	C→T	intergenic (‑67/+28)	pyrE ← / ← rph	orotate phosphoribosyltransferase/truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,835	0	C	T	100.0%	284.1 / NA	81	intergenic (‑67/+28)	pyrE/rph	orotate phosphoribosyltransferase/truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/41);  total (40/41)

AAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGC  >  NC_000913/3815700‑3815963                                                                                                                                        |                                                                                                                                  aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGtt                                                                                                                               >  2:1250605/1‑140 (MQ=255)   aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTAATTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTg                                                                                                                             >  1:1812317/1‑140 (MQ=255)       gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAg                                                                                                                         >  1:1201625/1‑140 (MQ=255)        ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAgc                                                                                                                        >  2:1502937/1‑140 (MQ=255)           aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGcgcc                                                                                                                     <  2:1927488/140‑1 (MQ=255)           aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGcgcc                                                                                                                     <  1:1980007/140‑1 (MQ=255)                   cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTg                                                                                                             <  2:380779/140‑1 (MQ=255)                   cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTg                                                                                                             <  2:2013008/140‑1 (MQ=255)                    acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTAATTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGc                                                                                                            <  2:1812317/140‑1 (MQ=255)                     ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCg                                                                                                           <  2:1525857/140‑1 (MQ=255)                      cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCg                                                                                                           >  1:2330187/1‑139 (MQ=255)                      cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCg                                                                                                           <  2:2330187/139‑1 (MQ=255)                      cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                                          <  1:646451/140‑1 (MQ=255)                      cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                                          <  2:1667279/140‑1 (MQ=255)                      cTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                                          <  2:1201625/140‑1 (MQ=255)                       tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                                         >  1:1784976/1‑140 (MQ=255)                       tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCATCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTc                                                                                                         >  2:2367061/1‑140 (MQ=255)                        gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                                                                        >  1:1569170/1‑140 (MQ=255)                        gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                                                                        >  2:2366793/1‑140 (MQ=255)                        gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCg                                                                                                        >  1:2158779/1‑140 (MQ=255)                              tAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACaa                                                                                                  >  2:1039057/1‑140 (MQ=255)                                  cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTg                                                                                                             <  1:218476/125‑1 (MQ=255)                                  cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTg                                                                                                             >  2:218476/1‑125 (MQ=255)                                  cgcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                                                                              >  2:1903120/1‑140 (MQ=255)                                   gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                             >  1:2577527/1‑140 (MQ=255)                                   gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgat                                                                                             <  2:930734/140‑1 (MQ=255)                                    cAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgatt                                                                                            <  2:1212114/140‑1 (MQ=255)                                       aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                                         >  2:1494232/1‑140 (MQ=255)                                       aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                                         >  1:1389576/1‑140 (MQ=255)                                          cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt‑c                                                                                     <  2:1674393/140‑1 (MQ=255)                                               aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑ccccct                                                                                <  1:1494232/140‑3 (MQ=255)                                               aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑ccccct                                                                                <  2:46249/140‑3 (MQ=255)                                                  tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑ccccctcgg                                                                             >  1:1535607/1‑135 (MQ=255)                                                   ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCACCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCggg                                                                             >  1:713432/1‑140 (MQ=255)                                                     cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGcc                                                                           <  2:973208/140‑1 (MQ=255)                                                      gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCa                                                                          <  2:1569170/140‑1 (MQ=255)                                                         gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCagag                                                                       <  2:908525/140‑1 (MQ=255)                                                         gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCagag                                                                       <  1:2553823/140‑1 (MQ=255)                                                          atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCaga                                                                        >  2:2616539/1‑138 (MQ=255)                                                          atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCaga                                                                        <  1:2616539/138‑1 (MQ=255)                                                          atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGc                                                                      >  2:137466/1‑140 (MQ=255)                                                           tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGcc                                                                     >  1:586231/1‑140 (MQ=255)                                                                  tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGa                                                              >  1:16379/1‑140 (MQ=255)                                                                    aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAg                                                               <  1:1249472/137‑1 (MQ=255)                                                                    aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAg                                                               >  2:1249472/1‑137 (MQ=255)                                                                    aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                            >  2:1243543/1‑140 (MQ=255)                                                                    aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                            >  1:2431690/1‑140 (MQ=255)                                                                     tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGa                                                           >  2:326187/1‑140 (MQ=255)                                                                      gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                          <  2:16379/140‑1 (MQ=255)                                                                         ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                       <  1:137466/140‑1 (MQ=255)                                                                         ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                       <  2:586231/140‑1 (MQ=255)                                                                         ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                       <  1:1903120/140‑1 (MQ=255)                                                                         ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                       <  1:2468882/140‑1 (MQ=255)                                                                         ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                       <  2:1784976/140‑1 (MQ=255)                                                                          tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGc                                                      >  1:1003733/1‑140 (MQ=255)                                                                              tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                  <  2:1389576/140‑1 (MQ=255)                                                                                ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                          <  2:1765268/130‑1 (MQ=255)                                                                                ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                          >  1:1765268/1‑130 (MQ=255)                                                                                        aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                        >  2:2157724/1‑140 (MQ=255)                                                                                             tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                   <  2:2329715/140‑1 (MQ=255)                                                                                             tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGACGAGTAGCTCTTCATGGGTGAACGGCt                                   >  2:2456986/1‑140 (MQ=255)                                                                                              cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                  >  1:619846/1‑140 (MQ=255)                                                                                              cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                  >  1:2660326/1‑140 (MQ=255)                                                                                               tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                 >  2:1834775/1‑140 (MQ=255)                                                                                                   gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                             <  2:619846/140‑1 (MQ=255)                                                                                                   gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                             <  2:2660326/140‑1 (MQ=255)                                                                                                    aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                        <  1:324903/128‑1 (MQ=255)                                                                                                    aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                        >  2:324903/1‑128 (MQ=255)                                                                                                      aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                       >  2:1551805/1‑127 (MQ=255)                                                                                                      aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                       <  1:1551805/127‑1 (MQ=255)                                                                                                        aaaaaaGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                        >  2:1539068/1‑140 (MQ=255)                                                                                                        aaaaaaGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                        <  1:1243543/140‑1 (MQ=255)                                                                                                        aaaaaaGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATCGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                        >  1:986865/1‑140 (MQ=255)                                                                                                         aaaaaGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCg                       <  1:2367061/140‑1 (MQ=255)                                                                                                           aaaGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                     <  2:1535607/140‑1 (MQ=255)                                                                                                              ggCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                  <  1:1022876/140‑1 (MQ=255)                                                                                                                 gtcTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc               <  2:1888493/138‑1 (MQ=255)                                                                                                                     catcaGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACtt           <  2:2577527/140‑1 (MQ=255)                                                                                                                             gCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg   <  1:2157724/140‑1 (MQ=255)                                                                                                                              ccTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc  >  2:2004969/1‑140 (MQ=255)                                                                                                                              ccTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc  >  1:2486448/1‑140 (MQ=255)                                                                                                                              ccTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc  >  1:791538/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  AAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGC  >  NC_000913/3815700‑3815963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑