BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,066,191	G→T	intergenic (‑427/+114)	cobU ← / ← insH‑6	cobinamide‑P guanylyltransferase/cobinamide kinase/CP4‑44 prophage; IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,066,191	0	G	T	100.0%	594.6 / NA	183	intergenic (‑427/+114)	cobU/insH‑6	cobinamide‑P guanylyltransferase/cobinamide kinase/CP4‑44 prophage; IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (105/78); total (105/78)

Only 100 of 187 total aligned reads displayed.

GAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAT  >  NC_000913/2066055‑2066314

gAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTAGCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATGCTTCTCTGCt                                                                                                                          <  2:1068431/140‑1 (MQ=255)

 aaaTCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTACGAATAAGTGGGGAAATTCTTCTctgctg                                                                                                                         <  1:369264/140‑1 (MQ=255)

    tCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACt                                                                                                                      >  1:1141419/1‑140 (MQ=255)

      cAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCa                                                                                                                    >  2:2369301/1‑140 (MQ=255)

         gCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtc                                                                                                                 >  2:1559268/1‑140 (MQ=255)

         gCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtc                                                                                                                 >  1:2150132/1‑140 (MQ=255)

         gCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtc                                                                                                                 >  1:1380595/1‑140 (MQ=255)

         gCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtc                                                                                                                 >  2:2431206/1‑140 (MQ=255)

              tGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtcatttc                                                                                                            <  1:2770687/140‑1 (MQ=255)

              tGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtcatttc                                                                                                            <  2:2072444/140‑1 (MQ=255)

              tGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtcatttc                                                                                                            >  1:2030724/1‑140 (MQ=255)

              tGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtcatttc                                                                                                            <  1:882538/140‑1 (MQ=255)

                aTTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGtcatttcat                                                                                                          <  1:1559268/140‑1 (MQ=255)

                     aCGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGt                                                                                                                  <  2:708046/127‑1 (MQ=255)

                     aCGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGt                                                                                                                  >  1:708046/1‑127 (MQ=255)

                     aCGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATttctt                                                                                                     >  1:490609/1‑140 (MQ=255)

                       gTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCa                                                                                                   >  2:2745835/1‑140 (MQ=255)

                       gTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCa                                                                                                   >  2:1103406/1‑140 (MQ=255)

                          aGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATttctt                                                                                                     <  2:1979651/135‑1 (MQ=255)

                          aGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGt                                                                                                >  1:1801381/1‑140 (MQ=255)

                                aGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                                          <  1:2767901/140‑1 (MQ=255)

                                 gATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                                                                                         <  2:2508456/140‑1 (MQ=255)

                                         cTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                                 >  2:346765/1‑140 (MQ=255)

                                         cTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                                 <  1:390800/140‑1 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAg                                                                                           >  1:2383297/1‑127 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                                                                                         >  1:849946/1‑129 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                                 <  2:1369091/137‑1 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                                 >  1:1369091/1‑137 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                                              >  1:250394/1‑140 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                                              >  2:2058538/1‑140 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                                              >  2:2132645/1‑140 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                                              >  2:234489/1‑140 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                                              >  1:922936/1‑140 (MQ=255)

                                            tAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                                              >  1:330898/1‑140 (MQ=255)

                                                 gAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTa                                                                         >  1:859567/1‑140 (MQ=255)

                                                  aaCTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaa                                                                        <  1:2745835/140‑1 (MQ=255)

                                                  aaCTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaa                                                                        <  2:1801381/140‑1 (MQ=255)

                                                   aCTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                                       <  1:2431206/140‑1 (MQ=255)

                                                     ttGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATg                                                                     >  2:2789783/1‑140 (MQ=255)

                                                      tGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGc                                                                    >  1:265764/1‑140 (MQ=255)

                                                            acaacGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              >  2:934744/1‑140 (MQ=255)

                                                            acaacGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              <  1:1086891/140‑1 (MQ=255)

                                                            acaacGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              <  2:850250/140‑1 (MQ=255)

                                                            acaacGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              <  2:2834307/140‑1 (MQ=255)

                                                            acaacGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              <  2:2706448/140‑1 (MQ=255)

                                                            acaacGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              <  1:234489/140‑1 (MQ=255)

                                                               acGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                                           >  1:516868/1‑140 (MQ=255)

                                                                cGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAg                                                          >  2:202686/1‑140 (MQ=255)

                                                                 gTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              <  1:2060372/135‑1 (MQ=255)

                                                                 gTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                                         <  2:490609/140‑1 (MQ=255)

                                                                 gTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                                         <  2:1291184/140‑1 (MQ=255)

                                                                 gTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                                         <  2:1380595/140‑1 (MQ=255)

                                                                 gTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                                         <  2:2150132/140‑1 (MQ=255)

                                                                  tAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              <  1:1946884/134‑1 (MQ=255)

                                                                  tAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                              >  2:1946884/1‑134 (MQ=255)

                                                                        aaTTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCAATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                                                  >  2:1221978/1‑140 (MQ=255)

                                                                          ttATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGa                                                >  2:290001/1‑140 (MQ=255)

                                                                          ttATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGa                                                >  1:1994293/1‑140 (MQ=255)

                                                                            aTCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                                              >  1:1394307/1‑140 (MQ=255)

                                                                             tCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCg                                             >  2:1441899/1‑140 (MQ=255)

                                                                             tCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCg                                             >  2:2503672/1‑140 (MQ=255)

                                                                             tCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCg                                             >  1:2204874/1‑140 (MQ=255)

                                                                                  aaTGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGcc                                                        <  1:1964991/124‑1 (MQ=255)

                                                                                  aaTGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAc                                               <  1:2564862/133‑1 (MQ=255)

                                                                                  aaTGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAc                                               >  2:2564862/1‑133 (MQ=255)

                                                                                   aTGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttctt                                       <  2:2204874/140‑1 (MQ=255)

                                                                                     ggtggCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                                     <  1:1657895/140‑1 (MQ=255)

                                                                                     ggtggCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                                     <  1:290001/140‑1 (MQ=255)

                                                                                     ggtggCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                                     <  1:346765/140‑1 (MQ=255)

                                                                                      gtggCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc                                    <  2:1976321/140‑1 (MQ=255)

                                                                                      gtggCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc                                    <  1:158168/140‑1 (MQ=255)

                                                                                            aCTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTa                              >  2:1960173/1‑140 (MQ=255)

                                                                                                tGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttctt                                       >  2:1601433/1‑127 (MQ=37)

                                                                                                tGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc                          >  2:2024799/1‑140 (MQ=37)

                                                                                                tGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc                          >  2:1125774/1‑140 (MQ=37)

                                                                                                 gCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg                         <  2:76964/140‑1 (MQ=25)

                                                                                                   aCTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt                       <  1:934744/140‑1 (MQ=18)

                                                                                                   aCTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt                       <  1:1617400/140‑1 (MQ=18)

                                                                                                      aGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATc                    >  1:1118386/1‑140 (MQ=18)

                                                                                                       gggAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc                   >  2:1915917/1‑140 (MQ=17)

                                                                                                       gggAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc                   >  1:838549/1‑140 (MQ=17)

                                                                                                       gggAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc                   <  2:838549/140‑1 (MQ=17)

                                                                                                       gggAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc                   <  1:1978088/140‑1 (MQ=17)

                                                                                                       gggAAGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc                   >  2:2408523/1‑140 (MQ=17)

                                                                                                          aaGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcccc                 <  1:1094420/139‑1 (MQ=17)

                                                                                                          aaGGTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCa                >  1:2213391/1‑140 (MQ=17)

                                                                                                            ggTGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGt              >  1:248978/1‑140 (MQ=17)

                                                                                                              tGCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGttt            <  2:270211/140‑1 (MQ=17)

                                                                                                               gCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg                         >  1:1858179/1‑126 (MQ=18)

                                                                                                               gCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGtttt           >  1:1823254/1‑140 (MQ=17)

                                                                                                               gCGAATAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGtttt           >  2:2439/1‑140 (MQ=17)

                                                                                                                   aTAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt       <  2:2770073/140‑1 (MQ=17)

                                                                                                                   aTAAGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt       <  1:1915917/140‑1 (MQ=17)

                                                                                                                      aGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgag    <  2:39104/140‑1 (MQ=17)

                                                                                                                      aGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgag    <  1:2541080/140‑1 (MQ=17)

                                                                                                                      aGTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgag    <  2:1842813/140‑1 (MQ=17)

                                                                                                                       gTGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgaga   >  2:2788522/1‑140 (MQ=14)

                                                                                                                        tGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAt  <  2:184195/140‑1 (MQ=11)

                                                                                                                        tGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAt  <  1:2132645/140‑1 (MQ=11)

                                                                                                                        tGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAt  >  2:1888239/1‑140 (MQ=11)

GAAATCCAGGCTATTGATTCAACGTCAGCGACAGATCTGCGCTGTAAAAGAACTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATGCACTAGGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAT  >  NC_000913/2066055‑2066314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`