BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,719,644	C→A	P141Q (CCG→CAG)	yfiP →	conserved protein YfiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,719,644	0	C	A	99.4%	587.0 / ‑3.3	161	P141Q (CCG→CAG)	yfiP	conserved protein YfiP
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (88/72); total (89/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

Only 100 of 162 total aligned reads displayed.

GCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAG  >  NC_000913/2719507‑2719778

     aGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCt                                                                                                                                 >  2:1287550/1‑140 (MQ=255)

         ggTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCa                                                                                                                             >  2:2255828/1‑140 (MQ=255)

         ggTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCa                                                                                                                             >  2:2016035/1‑140 (MQ=255)

           tGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACCCCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAACTCGCAAg                                                                                                                           >  1:2119305/1‑140 (MQ=255)

           tGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAg                                                                                                                           <  2:1465185/140‑1 (MQ=255)

            gCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGa                                                                                                                          >  1:2062575/1‑140 (MQ=255)

              aaaaCCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATg                                                                                                                        <  2:293108/140‑1 (MQ=255)

              aaaaCCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATg                                                                                                                        <  1:2342709/140‑1 (MQ=255)

               aaaCCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGt                                                                                                                       >  1:2865064/1‑140 (MQ=255)

                     ggACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTa                                                                                                                 <  2:1333148/140‑1 (MQ=255)

                      gACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTaa                                                                                                                <  1:1575425/140‑1 (MQ=255)

                           tCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTc                                                                                                           <  2:947366/140‑1 (MQ=255)

                           tCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTc                                                                                                           <  1:2080871/140‑1 (MQ=255)

                           tCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTc                                                                                                           <  2:2227488/140‑1 (MQ=255)

                           ccAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGAAGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTc                                                                                                           <  2:2607862/139‑1 (MQ=255)

                               ccAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTa                                                                                                       <  2:2091589/140‑1 (MQ=255)

                               ccAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTa                                                                                                       <  2:2378119/140‑1 (MQ=255)

                               ccAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTa                                                                                                       >  2:1391960/1‑140 (MQ=255)

                               ccAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTa                                                                                                       <  1:2829772/140‑1 (MQ=255)

                               ccAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTa                                                                                                       <  2:856831/140‑1 (MQ=255)

                                cAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTAt                                                                                                      <  1:1391960/140‑1 (MQ=255)

                                 aaTGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATc                                                                                                     >  2:674175/1‑140 (MQ=255)

                                   tggtggTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTg                                                                                                   >  2:2512831/1‑140 (MQ=255)

                                   tggtggTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTg                                                                                                   >  1:2864156/1‑140 (MQ=255)

                                     gtggtCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGa                                                                                                 <  1:2745710/140‑1 (MQ=255)

                                     gtggtCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGa                                                                                                 <  1:2703813/140‑1 (MQ=255)

                                     gtggtCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGa                                                                                                 >  1:685271/1‑140 (MQ=255)

                                     gtggtCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGa                                                                                                 >  1:1043263/1‑140 (MQ=255)

                                     gtggtCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGa                                                                                                 >  2:1534412/1‑140 (MQ=255)

                                        gtCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATaa                                                                                              >  1:1343614/1‑140 (MQ=255)

                                         tCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAAt                                                                                             >  2:1093966/1‑140 (MQ=255)

                                            ttCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCtt                                                                                          <  1:1962493/140‑1 (MQ=255)

                                               ccGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTccc                                                                                       <  1:674175/140‑1 (MQ=255)

                                                cGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTAt                                                                                                      >  1:302083/1‑124 (MQ=255)

                                                  cTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTc                                                                                    >  1:2779475/1‑140 (MQ=255)

                                                   ttCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCa                                                                                   <  1:1708435/140‑1 (MQ=255)

                                                   ttCGTATGCTGATGAGCAACGGGAAGTGATCTCCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCa                                                                                   <  1:1175183/140‑1 (MQ=255)

                                                         tGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCg                                                                             <  2:462740/140‑1 (MQ=255)

                                                             gatgaGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGa                                                                         >  2:2510287/1‑140 (MQ=255)

                                                              atgaGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGAt                                                                        <  2:2779475/140‑1 (MQ=255)

                                                               tgaGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATc                                                                       >  2:167840/1‑140 (MQ=255)

                                                                 aGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCtt                                                                     <  1:167840/140‑1 (MQ=255)

                                                                   cAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTc                                                                   >  1:2359019/1‑140 (MQ=255)

                                                                      cGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCg                                                                >  1:1530612/1‑140 (MQ=255)

                                                                      cGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCg                                                                <  1:1071424/140‑1 (MQ=255)

                                                                      cGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCg                                                                >  2:2800338/1‑140 (MQ=255)

                                                                        ggAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGc                                                              >  2:477574/1‑140 (MQ=255)

                                                                                   tcccaccacCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTAt                                                   <  2:114209/137‑1 (MQ=255)

                                                                                       accaccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGcc                                               <  1:895255/140‑1 (MQ=255)

                                                                                       accaccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGcc                                               >  2:2590112/1‑140 (MQ=255)

                                                                                       accaccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGcc                                               >  1:2469917/1‑140 (MQ=255)

                                                                                       accaccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGcc                                               >  1:2806253/1‑140 (MQ=255)

                                                                                          accTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGc                                            >  1:439849/1‑140 (MQ=255)

                                                                                           ccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                                           >  2:747786/1‑140 (MQ=255)

                                                                                           ccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                                           >  1:2297158/1‑140 (MQ=255)

                                                                                           ccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                                           >  1:2268611/1‑140 (MQ=255)

                                                                                           ccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                                           >  2:2520559/1‑140 (MQ=255)

                                                                                           ccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                                           >  1:1272158/1‑140 (MQ=255)

                                                                                           ccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                                           >  1:1312733/1‑140 (MQ=255)

                                                                                           ccTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                                           >  1:651832/1‑140 (MQ=255)

                                                                                            cTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGt                                          <  2:2677809/140‑1 (MQ=255)

                                                                                            cTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGt                                          >  1:2154080/1‑140 (MQ=255)

                                                                                            cTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGt                                          <  1:80914/140‑1 (MQ=255)

                                                                                             tGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCATCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTg                                         <  2:2737354/140‑1 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTg                                         >  2:1664212/1‑139 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTg                                         <  1:1664212/139‑1 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGa                                        >  1:1405968/1‑140 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGa                                        >  1:2517632/1‑140 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGa                                        >  2:1897623/1‑140 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGa                                        >  1:529522/1‑140 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGa                                        >  2:1117499/1‑140 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGa                                        >  2:164954/1‑140 (MQ=255)

                                                                                              gCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCATATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGCCTTTCTGCCTATCGCCTGCGTGa                                        >  2:819037/1‑140 (MQ=255)

                                                                                                     aGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAg                                 >  1:172822/1‑140 (MQ=255)

                                                                                                         aCCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTg                             >  1:1828990/1‑140 (MQ=255)

                                                                                                         aCCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTg                             >  2:318547/1‑140 (MQ=255)

                                                                                                         aCCGCTGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTg                             <  2:2335381/140‑1 (MQ=255)

                                                                                                              tGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTggcctt                        <  1:835848/140‑6 (MQ=255)

                                                                                                              tGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        <  2:509595/140‑1 (MQ=255)

                                                                                                              tGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        <  2:2731038/140‑1 (MQ=255)

                                                                                                              tGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        <  1:2188004/140‑1 (MQ=255)

                                                                                                              tGTTTATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        <  2:2852799/140‑1 (MQ=255)

                                                                                                              tGTTTATCACGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        <  2:1343614/140‑1 (MQ=255)

                                                                                                                 ttATCATGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCaa                     >  2:1837965/1‑140 (MQ=255)

                                                                                                                      aTGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTg                <  2:651832/140‑1 (MQ=255)

                                                                                                                       tGCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGt               >  1:1627539/1‑140 (MQ=255)

                                                                                                                        gCTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTa              <  2:1812607/140‑1 (MQ=255)

                                                                                                                         cTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTAc             <  2:854268/140‑1 (MQ=255)

                                                                                                                         cTCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTAc             >  2:2070015/1‑140 (MQ=255)

                                                                                                                          tCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        <  1:479266/128‑1 (MQ=255)

                                                                                                                          tCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        <  2:1223964/128‑1 (MQ=255)

                                                                                                                          tCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACt            >  1:2680697/1‑140 (MQ=255)

                                                                                                                          tCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACt            >  2:694650/1‑140 (MQ=255)

                                                                                                                          tCGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACt            >  1:1875784/1‑140 (MQ=255)

                                                                                                                           cGATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTg           <  2:2062575/140‑1 (MQ=255)

                                                                                                                            gATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCaa                     >  2:1879669/1‑129 (MQ=255)

                                                                                                                            gATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCaa                     <  1:1879669/129‑1 (MQ=255)

                                                                                                                            gATGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGc          >  2:2879939/1‑140 (MQ=255)

                                                                                                                              tGGTACCTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGc                        >  1:1141802/1‑124 (MQ=255)

                                                                                                                                    cTGGCAGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAg  >  1:1679966/1‑140 (MQ=255)

GCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAG  >  NC_000913/2719507‑2719778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`