Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,272,933	G→T	H209N (CAC→AAC)	garL ←	alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,272,933	0	G	T	100.0%	249.2 / NA	69	H209N (CAC→AAC)	garL	alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/33);  total (36/33)

ATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGTAGCGGCAA  >  NC_000913/3272802‑3273067                                                                                                                                    |                                                                                                                                       aTCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTgg                                                                                                                                >  2:1635622/1‑140 (MQ=255) aTCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTgg                                                                                                                                >  2:792989/1‑140 (MQ=255) aTCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTgg                                                                                                                                <  1:962685/140‑1 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGTGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  1:1993722/140‑1 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               >  1:1002574/1‑140 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  1:1172018/140‑1 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  1:2354775/140‑1 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  1:1965400/140‑1 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  1:336020/140‑1 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  1:183356/140‑1 (MQ=255)  tCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  2:2498030/140‑1 (MQ=255)   cAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCa                                                                                                                              <  2:1002574/140‑1 (MQ=255)    aGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCAc                                                                                                                             <  1:712517/140‑1 (MQ=255)         gTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               <  1:1507887/133‑1 (MQ=255)         gTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGc                                                                                                                               >  2:1507887/1‑133 (MQ=255)         gTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTa                                                                                                                        >  1:601785/1‑140 (MQ=255)          tttCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTaa                                                                                                                       >  2:285181/1‑140 (MQ=255)          tttCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTaa                                                                                                                       >  2:41842/1‑140 (MQ=255)          tttCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTaa                                                                                                                       >  2:841747/1‑140 (MQ=255)          tttCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTaa                                                                                                                       >  2:1417614/1‑140 (MQ=255)            tCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTaaaa                                                                                                                     >  2:2415082/1‑140 (MQ=255)                 gTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAAtgtg                                                                                                                <  1:2415082/140‑1 (MQ=255)                 gTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACATCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAAtgtg                                                                                                                <  1:1636589/140‑1 (MQ=255)                   ggCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCt                                                                                                              <  1:1623449/140‑1 (MQ=255)                    gCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTg                                                                                                             >  2:1710631/1‑140 (MQ=255)                    gCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTg                                                                                                             >  2:848351/1‑140 (MQ=255)                    gCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTg                                                                                                             >  2:442799/1‑140 (MQ=255)                     cAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGCGGATACCGCTGGGTTTGCCGATCGCGCTGGCACGGTTAAAAATGTGCTGa                                                                                                            >  2:2522506/1‑140 (MQ=255)                     cAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGa                                                                                                            <  1:77374/140‑1 (MQ=255)                        agCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAAtt                                                                                                         <  2:2228453/140‑1 (MQ=255)                          cGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGc                                                                                                       >  2:1865849/1‑140 (MQ=255)                           ggAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCt                                                                                                      <  1:1078094/140‑1 (MQ=255)                                 cGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGt                                                                                                <  1:209528/140‑1 (MQ=255)                                 cGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGt                                                                                                <  2:1383298/140‑1 (MQ=255)                                 cGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGt                                                                                                <  2:1065108/140‑1 (MQ=255)                                       gaTCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATcc                                                                                          <  1:1417614/140‑1 (MQ=255)                                       gaTCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATcc                                                                                          <  2:1589869/140‑1 (MQ=255)                                                 gACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATg                                                                                <  1:2810672/140‑1 (MQ=255)                                                       cacaAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGc                                                                          <  1:1865849/140‑1 (MQ=255)                                                            aCGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCgaga                                                                     <  1:658736/140‑1 (MQ=255)                                                            aCGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCgaga                                                                     <  1:207331/140‑1 (MQ=255)                                                              gTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATg                                                                   >  1:2234640/1‑140 (MQ=255)                                                              gTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATg                                                                   <  1:490334/140‑1 (MQ=255)                                                                cgcgCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCgag                                                                      >  1:1651969/1‑135 (MQ=255)                                                                cgcgCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCgag                                                                      <  2:1651969/135‑1 (MQ=255)                                                                          tCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCcgc                                                       <  1:2236551/140‑1 (MQ=255)                                                                                  acgacgCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGCTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGAt                                               >  2:2353939/1‑140 (MQ=255)                                                                                   cgacgCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATc                                              >  1:842824/1‑140 (MQ=255)                                                                                   cgacgCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATc                                              >  1:2319345/1‑140 (MQ=255)                                                                                   cgacgCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATc                                              >  2:2581326/1‑140 (MQ=255)                                                                                    gacgCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCg                                             >  1:2087345/1‑140 (MQ=255)                                                                                      cgcgCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCt                                           >  2:1760909/1‑140 (MQ=255)                                                                                      cgcgCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCt                                           >  1:1995435/1‑140 (MQ=255)                                                                                      cgcgCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCt                                           >  1:1329355/1‑140 (MQ=255)                                                                                      cgcgCATCGGCTTCGACCAGCGCGAGGATAACGATGGGTTTGCCGTTAGCGCTGGCACGGTTAAAAACGGGCTGAATTGCTTTTTGTACATCCGGGTGAGATGCATTACCGAGATGGACTAATGCCGCGGCCAGATCGCt                                           >  2:516191/1‑140 (MQ=255)                                                                                        cgcATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTgg                                         >  2:1665080/1‑140 (MQ=255)                                                                                        cgcATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTgg                                         >  1:607730/1‑140 (MQ=255)                                                                                        cgcATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTACTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTgg                                         >  2:2887528/1‑140 (MQ=255)                                                                                         gcATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTggg                                        <  1:2392689/140‑1 (MQ=255)                                                                                         gcATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTggg                                        <  1:742983/140‑1 (MQ=255)                                                                                                   cGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCTGCGGCCAGATCGCTGGGGCCGACGAAg                              >  1:1380827/1‑140 (MQ=255)                                                                                                   cGACCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTACTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAg                              <  1:2887528/140‑1 (MQ=255)                                                                                                     aCCGGCGCGAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGAt                            >  1:2144611/1‑140 (MQ=255)                                                                                                          cgcgAGGATACCGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGt                       >  2:2046/1‑140 (MQ=255)                                                                                                                    ccGCTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTc             >  2:386535/1‑140 (MQ=255)                                                                                                                       cTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGt          >  2:304222/1‑140 (MQ=255)                                                                                                                       cTGGGTTTGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGt          <  1:2160940/140‑1 (MQ=255)                                                                                                                            tttGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGTAGCgg     >  2:2377939/1‑140 (MQ=255)                                                                                                                            tttGCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGTAGCgg     >  1:1458776/1‑140 (MQ=255)                                                                                                                               gCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGTAGCGGCaa  >  2:2398327/1‑140 (MQ=255)                                                                                                                               gCCGTTCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGTAGCGGCaa  >  1:1092253/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       ATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGGCCTAATGCCGCGGCCAGATCGCTGGGGCCGACGAAGATGCCGTCTACGCCTTCGGTAGCGGCAA  >  NC_000913/3272802‑3273067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑