Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,301,945	G→T	R154L (CGG→CTG)	yhbU →	putative peptidase YhbU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,301,945	0	G	T	100.0%	342.3 / NA	95	R154L (CGG→CTG)	yhbU	putative peptidase YhbU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (51/44);  total (51/44)

CATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTC  >  NC_000913/3301809‑3302078                                                                                                                                         |                                                                                                                                      cATATTGAGGGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTATCTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCAATTCATCAGGTGAAACAACTGGCACTGGt                                                                                                                                    <  2:1426544/140‑1 (MQ=255) cATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGt                                                                                                                                    <  2:2298874/140‑1 (MQ=255) cATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGt                                                                                                                                    <  1:1792062/140‑1 (MQ=255) cATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGt                                                                                                                                    <  2:1917022/140‑1 (MQ=255) cATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGt                                                                                                                                    <  2:2365482/140‑1 (MQ=255) cATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGt                                                                                                                                    <  2:271825/140‑1 (MQ=255) cATATTGAGCCCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGt                                                                                                                                    <  1:128599/140‑1 (MQ=255)  atatTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTc                                                                                                                                   >  1:2313769/1‑140 (MQ=255)      tGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCacac                                                                                                                               >  1:2493519/1‑140 (MQ=255)       gAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACAcc                                                                                                                              >  1:1822441/1‑140 (MQ=255)        aGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCt                                                                                                                             >  2:1825188/1‑140 (MQ=255)        aGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCt                                                                                                                             >  2:2048073/1‑140 (MQ=255)         gcgcCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTg                                                                                                                            <  1:1825188/140‑1 (MQ=255)           gcCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTa                                                                                                                          >  2:565732/1‑140 (MQ=255)            ccACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTAc                                                                                                                         <  2:2405208/140‑1 (MQ=255)             cACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTAcc                                                                                                                        >  1:2218959/1‑140 (MQ=255)               cGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCAc                                                                                                                      >  2:1905751/1‑140 (MQ=255)                 tATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTg                                                                                                                    <  1:2788053/140‑1 (MQ=255)                       tGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTc                                                                                                              >  2:1851472/1‑140 (MQ=255)                         cAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCtt                                                                                                            >  2:1165431/1‑140 (MQ=255)                         cAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCtt                                                                                                            >  1:2638928/1‑140 (MQ=255)                            gCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGc                                                                                                         <  2:1034335/140‑1 (MQ=255)                            gCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGc                                                                                                         <  1:1036572/140‑1 (MQ=255)                            gCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGc                                                                                                         >  1:122742/1‑140 (MQ=255)                                cGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTc                                                                                                     >  2:2854489/1‑140 (MQ=255)                                 ggCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCg                                                                                                    >  1:840003/1‑140 (MQ=255)                                      ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGc                                                                                               >  2:1684007/1‑140 (MQ=255)                                      ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGc                                                                                               <  1:1165431/140‑1 (MQ=255)                                      ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGc                                                                                               <  1:1131783/140‑1 (MQ=255)                                      ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGc                                                                                               <  1:273110/140‑1 (MQ=255)                                       cAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGcc                                                                                              <  1:2854489/140‑1 (MQ=255)                                        aaTGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCt                                                                                             <  1:1567083/140‑1 (MQ=255)                                        aaTGAAGAGGCGATTAACTATTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCGCACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCt                                                                                             <  1:2048073/140‑1 (MQ=255)                                            aaGAGGCGATTAACTTTTATCGTCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTATCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGc                                                                                         <  1:2852344/140‑1 (MQ=255)                                            aaGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGc                                                                                         <  1:2378995/140‑1 (MQ=255)                                             agagGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCa                                                                                        <  2:840003/140‑1 (MQ=255)                                             agagGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCa                                                                                        <  1:1687463/140‑1 (MQ=255)                                              gagGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCAt                                                                                       <  1:2695091/140‑1 (MQ=255)                                                 gCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTAt                                                                                    >  1:2702963/1‑140 (MQ=255)                                                 gCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTAt                                                                                    >  1:21285/1‑140 (MQ=255)                                                 gCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTAt                                                                                    <  1:98414/140‑1 (MQ=255)                                                 gCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTAt                                                                                    >  2:741419/1‑140 (MQ=255)                                                  cGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATg                                                                                   >  2:1587067/1‑140 (MQ=255)                                                    aTTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTc                                                                                 >  1:284987/1‑140 (MQ=255)                                                    aTTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTc                                                                                 >  2:2329407/1‑140 (MQ=255)                                                    aTTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTc                                                                                 >  1:803868/1‑140 (MQ=255)                                                        aCTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGaa                                                                             <  1:1851472/140‑1 (MQ=255)                                                          ttttATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAgg                                                                           <  2:2702963/140‑1 (MQ=255)                                                           tttATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTCCATTATGTCGGAAGGt                                                                          <  2:1822441/140‑1 (MQ=255)                                                             tATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCg                                                                        >  1:1491592/1‑140 (MQ=255)                                                              atcatcGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGt                                                                       >  2:2855771/1‑140 (MQ=255)                                                                   cGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTa                                                                  >  2:2255023/1‑140 (MQ=255)                                                                   cGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTa                                                                  >  2:2588495/1‑140 (MQ=255)                                                                    gCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGa                                                                              <  1:2699206/127‑1 (MQ=255)                                                                    gCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGa                                                                              >  2:2699206/1‑127 (MQ=255)                                                                    gCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTAt                                                                 <  2:2218959/140‑1 (MQ=255)                                                                    gCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTAt                                                                 >  2:2206546/1‑140 (MQ=255)                                                                    gCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTAt                                                                 <  1:41152/140‑1 (MQ=255)                                                                    gCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTAt                                                                 >  2:1227573/1‑140 (MQ=255)                                                                    gCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGCGCATTATGTCGGAAGGTCGTTGCTAt                                                                 >  1:1027483/1‑140 (MQ=255)                                                                       aTTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTg                                                              <  1:1905751/140‑1 (MQ=255)                                                                         tttGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTgtc                                                            >  1:1053654/1‑140 (MQ=255)                                                                          ttGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTgtcg                                                           <  1:2329407/140‑1 (MQ=255)                                                                             aCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGtcgtcg                                                        >  1:2295497/1‑140 (MQ=255)                                                                             aCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGtcgtcg                                                        >  2:2295507/1‑140 (MQ=255)                                                                                ttGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTAt                                                     >  1:2563822/1‑140 (MQ=255)                                                                                ttGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGC‑GCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATc                                                    >  1:694392/1‑140 (MQ=255)                                                                                  gCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCt                                                   >  1:1724589/1‑140 (MQ=255)                                                                                       cgTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACgg                                              >  1:1255176/1‑140 (MQ=255)                                                                                           gtgCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATc                                                    <  2:1357475/130‑1 (MQ=255)                                                                                           gtgCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATc                                                    >  1:1357475/1‑130 (MQ=255)                                                                                           gtgCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGa                                          >  2:316274/1‑140 (MQ=255)                                                                                           gtgCCGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGa                                          >  1:2755746/1‑140 (MQ=255)                                                                                            tgctgcCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAg                                         <  1:1211296/140‑1 (MQ=255)                                                                                            tgctgcCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAg                                         <  1:1227573/140‑1 (MQ=255)                                                                                             gctgcCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGt                                        <  2:21285/140‑1 (MQ=255)                                                                                             gctgcCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGt                                        >  2:775170/1‑140 (MQ=255)                                                                                               tgcCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCg                                      <  2:649338/140‑1 (MQ=255)                                                                                                  cgcgcgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGccc                                   <  2:1724589/140‑1 (MQ=255)                                                                                                    cgcgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCaa                                 <  1:2206546/140‑1 (MQ=255)                                                                                                    cgcgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCaa                                 <  2:284987/140‑1 (MQ=255)                                                                                                      cgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAaca                               >  2:1012720/1‑140 (MQ=255)                                                                                                      cgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAaca                               >  1:2535037/1‑140 (MQ=255)                                                                                                      cgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAaca                               >  1:2382744/1‑140 (MQ=255)                                                                                                      cgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAaca                               >  1:293293/1‑140 (MQ=255)                                                                                                      cgTGTTGTCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAaca                               >  1:75702/1‑140 (MQ=255)                                                                                                           tgtCGATTCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATa                          <  2:1255176/140‑1 (MQ=255)                                                                                                                 ttCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATa                          >  2:168721/1‑134 (MQ=255)                                                                                                                 ttCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATa                          <  1:168721/134‑1 (MQ=255)                                                                                                                 ttCATCAGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGcgcg                    <  2:2638928/140‑1 (MQ=255)                                                                                                                     tcaGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGCGTCGCCCAACACCATAGGCGCGTGtt                >  2:1338362/1‑140 (MQ=255)                                                                                                                     tcaGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGtt                <  2:1491592/140‑1 (MQ=255)                                                                                                                       aGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtct              >  1:2039992/1‑140 (MQ=255)                                                                                                                       aGGTGAAACAACTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtct              <  2:75702/140‑1 (MQ=255)                                                                                                                              acaacTGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGccc       >  1:502767/1‑140 (MQ=255)                                                                                                                                   tGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTc  >  1:542227/1‑140 (MQ=255)                                                                                                                                   tGGCACTGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTc  >  2:1012863/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTC  >  NC_000913/3301809‑3302078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑