Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,218,270 G→T A388S (GCC→TCC)  aceA → isocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,218,2700GT98.7% 563.1 / ‑1.9 158A388S (GCC→TCC) aceAisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (77/79);  total (78/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 162 total aligned reads displayed.

TATGGGCTACAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCG  >  NC_000913/4218134‑4218404
                                                                                                                                        |                                                                                                                                      
tatGGGCTACAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTtccg                                                                                                                                     <  1:2793772/140‑1 (MQ=255)
  tGGGCTACAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTtccgcc                                                                                                                                   >  2:2299300/1‑140 (MQ=255)
     gCTACAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCcgcg                                                                                                                                >  1:2869429/1‑140 (MQ=255)
     gCTACAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCcgcg                                                                                                                                >  1:1249568/1‑140 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCCCTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  2:2855703/140‑1 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  2:1265510/140‑1 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  2:1696806/140‑1 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            >  1:745366/1‑140 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  1:302629/140‑1 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  1:2742670/140‑1 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            >  1:1380617/1‑140 (MQ=255)
         cAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  2:2869429/140‑1 (MQ=255)
           aGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGAt                                                                                                                          <  2:257652/140‑1 (MQ=255)
               ccAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGaaa                                                                                                                             >  2:1500729/1‑133 (MQ=255)
               ccAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCt                                                                                                                      >  2:887714/1‑140 (MQ=255)
               ccAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCt                                                                                                                      >  1:1098041/1‑140 (MQ=255)
                cAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCta                                                                                                                     >  2:2770579/1‑140 (MQ=255)
                cAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCta                                                                                                                     <  2:2750192/140‑1 (MQ=255)
                    tcatcaCCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  1:55008/129‑1 (MQ=255)
                     catcaCCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCt                                                                                                                <  1:2638266/140‑1 (MQ=255)
                       tcaCCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGGAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTc                                                                                                              >  1:496950/1‑140 (MQ=255)
                       tcaCCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            <  1:1764059/126‑1 (MQ=255)
                       tcaCCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAg                                                                                                                            >  2:1764059/1‑126 (MQ=255)
                       tcaCCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTc                                                                                                              >  2:1223297/1‑140 (MQ=255)
                         ccccTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGt                                                                                                            <  2:803708/139‑1 (MQ=255)
                         aCCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGt                                                                                                            <  2:2535189/140‑1 (MQ=255)
                           ccTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTAt                                                                                                          >  2:2123403/1‑140 (MQ=255)
                             tGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTAtct                                                                                                        >  2:1016519/1‑140 (MQ=255)
                               gCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCa                                                                                                      >  2:2507491/1‑140 (MQ=255)
                                 aGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCAcc                                                                                                    <  1:1044079/140‑1 (MQ=255)
                                  ggTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACca                                                                                                   >  1:1383793/1‑140 (MQ=255)
                                    tATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACcagc                                                                                                 <  2:1686382/140‑1 (MQ=255)
                                     aTCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACcagca                                                                                                >  1:2678350/1‑140 (MQ=255)
                                     aTCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACcagca                                                                                                >  1:12064/1‑140 (MQ=255)
                                      tCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACcagcag                                                                                               <  1:813430/140‑1 (MQ=255)
                                      tCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACcagcag                                                                                               <  2:1272840/140‑1 (MQ=255)
                                      tCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACcagcag                                                                                               >  2:2839073/1‑140 (MQ=255)
                                      tCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACcagcag                                                                                               <  1:494791/140‑1 (MQ=255)
                                         acaGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGaa                                                                                            >  2:1823591/1‑140 (MQ=255)
                                         acaGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGaa                                                                                            <  2:1465212/140‑1 (MQ=255)
                                         acaGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGaa                                                                                            <  2:129992/140‑1 (MQ=255)
                                          caGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAg                                                                                           <  1:1801536/140‑1 (MQ=255)
                                           aGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGt                                                                                          >  1:2639703/1‑140 (MQ=255)
                                             cATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTgg                                                                                        >  2:1346701/1‑140 (MQ=255)
                                               tgtgGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGt                                                                                      >  1:1003786/1‑140 (MQ=255)
                                                gtgGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGt                                                                                          <  2:146064/135‑1 (MQ=255)
                                                gtgGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGt                                                                                          >  2:545039/1‑135 (MQ=255)
                                                gtgGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGt                                                                                          >  1:146064/1‑135 (MQ=255)
                                                gtgGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCAGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTa                                                                                     >  2:2144546/1‑140 (MQ=255)
                                                  ggTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACa                                                                                   >  1:2310690/1‑140 (MQ=255)
                                                   gTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAg                                                                                  <  1:341351/140‑1 (MQ=255)
                                                   gTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAg                                                                                  <  1:1578396/140‑1 (MQ=255)
                                                       aaCATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTa                                                                              <  1:1346701/140‑1 (MQ=255)
                                                        aCATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTAc                                                                             >  2:733225/1‑140 (MQ=255)
                                                        aCATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTAc                                                                             <  1:1016519/140‑1 (MQ=255)
                                                              ttGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGAt                                                                       >  2:2077822/1‑140 (MQ=255)
                                                                   cTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGt                                                                  >  2:2594488/1‑140 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGTTTACTTCGATAAAGTgac                                                               <  1:548650/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  2:1225430/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:1223297/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:1256660/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:2484691/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  2:1249568/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:2582010/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:2625217/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:2770579/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:2839073/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  2:1098041/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  1:974241/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  2:1063934/140‑1 (MQ=255)
                                                                      gCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgac                                                               <  2:1375489/140‑1 (MQ=255)
                                                                       cAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGAACAGGTTACTTCGATAAAGTgacg                                                              >  2:2459447/1‑140 (MQ=255)
                                                                        aaaCGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgacga                                                             <  2:2634521/140‑1 (MQ=255)
                                                                           cGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACta                                                          <  2:1868652/140‑1 (MQ=255)
                                                                           cGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACta                                                          <  2:2818605/140‑1 (MQ=255)
                                                                           cGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACta                                                          <  2:2678350/140‑1 (MQ=255)
                                                                            gCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACtat                                                         >  2:583075/1‑140 (MQ=255)
                                                                                aTGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgacga                                                             >  1:1812535/1‑132 (MQ=255)
                                                                                aTGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTgacga                                                             <  2:1812535/132‑1 (MQ=255)
                                                                                aTGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTattatt                                                     >  2:819390/1‑140 (MQ=255)
                                                                                     cAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAggg                                                <  1:2123403/140‑1 (MQ=255)
                                                                                        ggCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTattatt                                                     <  1:2853017/132‑1 (MQ=255)
                                                                                        ggCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGggcgg                                             >  1:1653319/1‑140 (MQ=255)
                                                                                        ggCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGggcgg                                             >  2:410588/1‑140 (MQ=255)
                                                                                          cGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCa                                           <  1:942272/140‑1 (MQ=255)
                                                                                          cGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCa                                           <  1:52112/140‑1 (MQ=255)
                                                                                          cGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCa                                           <  1:2299300/140‑1 (MQ=255)
                                                                                             gggTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCa                                           >  1:2492592/1‑137 (MQ=255)
                                                                                             gggTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCa                                           <  2:2492592/137‑1 (MQ=255)
                                                                                             gggTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGt                                        >  1:1626469/1‑140 (MQ=255)
                                                                                                         cTACGTTGAGAAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGAACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACcg                            >  2:1749002/1‑140 (MQ=255)
                                                                                                                 agaAAGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGAcc                    >  2:2885274/1‑140 (MQ=255)
                                                                                                                   aaaGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCgg                  <  2:2639703/140‑1 (MQ=255)
                                                                                                                    aaGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGc                 >  1:1537812/1‑140 (MQ=255)
                                                                                                                     aGTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCt                <  1:733225/140‑1 (MQ=255)
                                                                                                                      gTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTc               >  2:2270839/1‑140 (MQ=255)
                                                                                                                      gTGCAGCAGCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTc               >  2:589987/1‑140 (MQ=255)
                                                                                                                         cagcagCCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCAc            >  1:1058562/1‑140 (MQ=255)
                                                                                                                              gcCGGAATTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTgaag       <  2:496950/140‑1 (MQ=255)
                                                                                                                                   aaTTTTCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCg  <  1:2513667/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TATGGGCTACAAGTTCCAGTTCATCACCCTGGCAGGTATCCACAGCATGTGGTTCAACATGTTTGACCTGGCAAACGCCTATGCCCAGGGCGAGGGTATGAAGCACTACGTTGAGAAAGTGCAGCAGCCGGAATTTGCCGCCGCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCG  >  NC_000913/4218134‑4218404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑