Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,880,107 G→T intergenic (‑159/+14) yeaR ← / ← leuE DUF1971 domain‑containing protein YeaR/leucine exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,880,1070GT98.8% 604.7 / ‑2.5 167intergenic (‑159/+14)yeaR/leuEDUF1971 domain‑containing protein YeaR/leucine exporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (91/74);  total (93/74)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

TGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGAT  >  NC_000913/1879970‑1880241
                                                                                                                                         |                                                                                                                                      
tGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAActcg                                                                                                                                      >  1:216181/1‑140 (MQ=255)
tGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAActcg                                                                                                                                      >  2:2086208/1‑140 (MQ=255)
      gAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGa                                                                                                                                >  2:1242256/1‑140 (MQ=255)
       aaaaCGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGaa                                                                                                                               <  2:1086942/140‑1 (MQ=255)
       aaaaCGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGaa                                                                                                                               <  1:1242256/140‑1 (MQ=255)
          aCGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGc                                                                                                                            <  1:1869213/140‑1 (MQ=255)
            ggTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCAt                                                                                                                          <  1:389020/140‑1 (MQ=255)
                      aGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCa                                                                                                                >  1:816209/1‑140 (MQ=255)
                        ccGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGc                                                                                                              >  2:2174973/1‑140 (MQ=255)
                        ccGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGc                                                                                                              >  1:1699314/1‑140 (MQ=255)
                                     cGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCggg                                                                                                 >  1:2095306/1‑140 (MQ=255)
                                     cGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCggg                                                                                                 >  2:1931392/1‑140 (MQ=255)
                                     cGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCggg                                                                                                 >  2:1280806/1‑140 (MQ=255)
                                           aTAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGa                                                                                           >  1:679323/1‑140 (MQ=255)
                                           aTAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGa                                                                                           >  1:2706223/1‑140 (MQ=255)
                                           aTAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGa                                                                                           >  2:2742374/1‑140 (MQ=255)
                                           aTAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGa                                                                                           <  2:2809805/140‑1 (MQ=255)
                                             aaTTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGaaa                                                                                         <  2:2095306/140‑1 (MQ=255)
                                             aaTTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGaaa                                                                                         <  2:530600/140‑1 (MQ=255)
                                                  gCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCAc                                                                                    >  2:1271613/1‑140 (MQ=255)
                                                   cATTCTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACg                                                                                   <  2:2706223/140‑1 (MQ=255)
                                                     ttttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGaa                                                                                 >  1:1924058/1‑140 (MQ=255)
                                                      tttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCgg                                                                                                  <  2:1433414/122‑1 (MQ=255)
                                                      tttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCgg                                                                                                  >  1:1433414/1‑122 (MQ=255)
                                                       ttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACa                                                                               <  2:860795/140‑1 (MQ=255)
                                                       ttAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACa                                                                               <  2:2330277/140‑1 (MQ=255)
                                                          aaTACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGaaa                                                                                         <  2:2704367/127‑1 (MQ=255)
                                                             aCCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAAc                                                                         <  2:2889117/140‑1 (MQ=255)
                                                                 tttATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAAt                                                                     >  1:1623906/1‑140 (MQ=255)
                                                                  ttATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATc                                                                    <  2:1623906/140‑1 (MQ=255)
                                                                        gTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAg                                                              >  2:545995/1‑140 (MQ=255)
                                                                         ttACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGt                                                             >  2:1481733/1‑140 (MQ=255)
                                                                         ttACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGt                                                             >  2:1934147/1‑140 (MQ=255)
                                                                           aCTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTg                                                           >  1:1966504/1‑140 (MQ=255)
                                                                           aCTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTg                                                           >  2:2786990/1‑140 (MQ=255)
                                                                           aCTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTg                                                           >  1:120765/1‑140 (MQ=255)
                                                                           aCTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTg                                                           >  1:1157642/1‑140 (MQ=255)
                                                                               tttAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCaa                                                       >  2:1343615/1‑140 (MQ=255)
                                                                                  aGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACtt                                                    >  1:2558018/1‑140 (MQ=255)
                                                                                  aGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACtt                                                    >  1:2052707/1‑140 (MQ=255)
                                                                                    cACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTa                                                  >  1:1211344/1‑140 (MQ=255)
                                                                                       cATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGcc                                               >  1:1038029/1‑140 (MQ=255)
                                                                                       cATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGACAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGcc                                               >  2:1398484/1‑140 (MQ=255)
                                                                                         tatCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGc                                                          <  2:1664293/127‑1 (MQ=255)
                                                                                         tatCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGc                                                          >  1:1664293/1‑127 (MQ=255)
                                                                                         tatCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAg                                             >  2:2485498/1‑140 (MQ=255)
                                                                                         tatCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAg                                             >  2:1455821/1‑140 (MQ=255)
                                                                                         tatCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAg                                             >  2:2561000/1‑140 (MQ=255)
                                                                                                 aagaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCtttt                                     <  1:2214129/140‑1 (MQ=255)
                                                                                                 aagaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCtttt                                     >  1:639443/1‑140 (MQ=255)
                                                                                                 aagaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCtttt                                     >  1:537396/1‑140 (MQ=255)
                                                                                                  agaaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCttttt                                    <  1:906109/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGTAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  2:2859055/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  1:1455821/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  1:1776095/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  2:174470/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  2:2391902/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  1:2284756/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  2:1699314/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  1:1273682/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  1:2561000/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  2:565211/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  1:2789319/140‑1 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  >  1:588199/1‑140 (MQ=255)
                                                                                                    aaTCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTgg                                  <  2:1206863/140‑1 (MQ=255)
                                                                                                      tCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTa                                <  1:2081602/140‑1 (MQ=255)
                                                                                                      tCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTa                                <  1:2508536/140‑1 (MQ=255)
                                                                                                      tCAGGGAGGATTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTa                                >  1:744534/1‑140 (MQ=255)
                                                                                                              gaTTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCttt                                      <  2:761530/126‑1 (MQ=255)
                                                                                                              gaTTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTa                        >  1:1841605/1‑140 (MQ=255)
                                                                                                              gaTTATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTa                        <  1:549401/140‑1 (MQ=255)
                                                                                                                ttATAGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGt                             >  2:1098367/1‑133 (MQ=255)
                                                                                                                 tataGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTg                     <  2:2558018/140‑1 (MQ=255)
                                                                                                                  ataGATGGGAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGc                    <  2:1841605/140‑1 (MQ=255)
                                                                                                                        gggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACTTCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa              >  1:764953/1‑140 (MQ=255)
                                                                                                                        gggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa              >  1:2410075/1‑140 (MQ=255)
                                                                                                                        gggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa              >  1:1366414/1‑140 (MQ=255)
                                                                                                                        gggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa              >  1:419338/1‑140 (MQ=255)
                                                                                                                        gggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa              >  1:902145/1‑140 (MQ=255)
                                                                                                                        gggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa              >  1:647311/1‑140 (MQ=255)
                                                                                                                        gggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAATTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACa              >  2:136188/1‑140 (MQ=255)
                                                                                                                         ggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaa             >  1:2847040/1‑140 (MQ=255)
                                                                                                                         ggAAGCCCGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaa             >  1:599779/1‑140 (MQ=255)
                                                                                                                              cccGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCa        <  2:2645203/140‑1 (MQ=255)
                                                                                                                              cccGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCa        <  2:659461/140‑1 (MQ=255)
                                                                                                                              cccGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCa        >  1:750465/1‑140 (MQ=255)
                                                                                                                               ccGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc       <  1:2867798/140‑1 (MQ=255)
                                                                                                                               ccGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc       <  2:1157642/140‑1 (MQ=255)
                                                                                                                               ccGCGACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc       <  1:2306846/140‑1 (MQ=255)
                                                                                                                                cgcgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaaa            <  1:1024698/134‑1 (MQ=255)
                                                                                                                                cgcgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaaa            >  2:1024698/1‑134 (MQ=255)
                                                                                                                                cgcgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAcc      >  2:482385/1‑140 (MQ=255)
                                                                                                                                cgcgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAcc      >  2:1273951/1‑140 (MQ=255)
                                                                                                                                cgcgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAcc      >  1:199784/1‑140 (MQ=255)
                                                                                                                                cgcgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCAc       <  2:1985091/139‑1 (MQ=255)
                                                                                                                                  cgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACaaa            <  2:2240236/132‑1 (MQ=255)
                                                                                                                                  cgACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAg    >  1:1414700/1‑140 (MQ=255)
                                                                                                                                   gACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGa   <  2:120765/140‑1 (MQ=255)
                                                                                                                                   gACAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGa   <  1:1292948/140‑1 (MQ=255)
                                                                                                                                    aCAACTCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGat  <  1:516143/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TGCGCTGAAAACGGTTTATTCCAGCCGTTTCAGGGTACGCCTGATAATTTGCATTTTAAATACCATTTATTGGTTACTTTTTAGCACCATATCAGCGAAGAATCAGGGAGGATTATAGATGGGAAGCCCGCGACAACGCGGGCTGAAAGCATCAGGATTGCAGCGTCGCCAGTCGGGCAGCGAAACCCACGAACATCAAACCAATCAGTGAGTTGCCAACTTTAGCCAGTTTCTTTTTGGTACGTATGTACTGCGTGACAAAAGCACCAGAT  >  NC_000913/1879970‑1880241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑