Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 95,913 G→T A422S (GCT→TCT)  murF → D‑alanyl‑D‑alanine‑adding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091395,9130GT100.0% 402.3 / NA 116A422S (GCT→TCT) murFD‑alanyl‑D‑alanine‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (54/62);  total (54/62)

Only 100 of 117 total aligned reads displayed.

GCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCT  >  NC_000913/95776‑96047
                                                                                                                                         |                                                                                                                                      
gCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCt                                                                                                                                      >  2:81169/1‑140 (MQ=255)
gCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCt                                                                                                                                      >  2:1490208/1‑140 (MQ=255)
 cGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTg                                                                                                                                     >  1:1694301/1‑140 (MQ=255)
 cGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTg                                                                                                                                     >  1:732978/1‑140 (MQ=255)
 cGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTg                                                                                                                                     >  2:1140447/1‑140 (MQ=255)
 cGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTg                                                                                                                                     >  2:1145684/1‑140 (MQ=255)
 cGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTg                                                                                                                                     >  2:1681464/1‑140 (MQ=255)
 cGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTg                                                                                                                                     >  1:1187703/1‑140 (MQ=255)
   aGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAg                                                                                                                                   <  1:81169/140‑1 (MQ=255)
   aGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAg                                                                                                                                   <  1:584438/140‑1 (MQ=255)
      cggcAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCaa                                                                                                                                >  2:1196039/1‑140 (MQ=255)
      cggcAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCaa                                                                                                                                <  2:1502621/140‑1 (MQ=255)
          aaaaGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAgg                                                                                                                            <  2:1694301/140‑1 (MQ=255)
                tgctgGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTa                                                                                                                      <  1:467535/140‑1 (MQ=255)
                tgctgGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTa                                                                                                                      <  1:956668/140‑1 (MQ=255)
                  ctgGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACg                                                                                                                    >  1:665584/1‑140 (MQ=255)
                     gTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGAtt                                                                                                                 <  2:1550663/140‑1 (MQ=255)
                          gACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGt                                                                                                            >  1:1455767/1‑140 (MQ=255)
                            ccGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTa                                                                                                          <  1:102927/140‑1 (MQ=255)
                            ccGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTa                                                                                                          <  1:168192/140‑1 (MQ=255)
                             cgcgTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTaa                                                                                                         <  1:238256/140‑1 (MQ=255)
                               cgTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAgg                                                                                                       >  2:87540/1‑140 (MQ=255)
                               cgTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAgg                                                                                                       >  1:418547/1‑140 (MQ=255)
                               cgTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAgg                                                                                                       >  2:459/1‑140 (MQ=255)
                               cgTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAgg                                                                                                       >  2:1249555/1‑140 (MQ=255)
                               cgTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAgg                                                                                                       >  1:498411/1‑140 (MQ=255)
                               cgTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAgg                                                                                                       >  1:1395297/1‑140 (MQ=255)
                                 tgtTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGt                                                                                                     <  1:1681464/140‑1 (MQ=255)
                                 tgtTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGt                                                                                                     <  2:1445272/140‑1 (MQ=255)
                                  gtTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGtt                                                                                                    <  1:364153/140‑1 (MQ=255)
                                    tAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCa                                                                                                  <  2:418547/140‑1 (MQ=255)
                                      aGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACg                                                                                                <  1:351075/140‑1 (MQ=255)
                                           gggTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTg                                                                                           >  2:753825/1‑140 (MQ=255)
                                            ggTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTgc                                                                                          <  1:459/140‑1 (MQ=255)
                                            ggTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTgc                                                                                          <  1:753825/140‑1 (MQ=255)
                                             gTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTgcc                                                                                         >  1:1877383/1‑140 (MQ=255)
                                             gTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTgcc                                                                                         >  2:1271719/1‑140 (MQ=255)
                                                  cAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCAt                                                                                    <  2:813533/140‑1 (MQ=255)
                                                    aaGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATgg                                                                                  <  2:1323139/140‑1 (MQ=255)
                                                    aaGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATgg                                                                                  <  2:1488371/140‑1 (MQ=255)
                                                     aGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTgccgcc                                                                                      <  1:331812/135‑1 (MQ=255)
                                                     aGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTgccgcc                                                                                      >  2:331812/1‑135 (MQ=255)
                                                     aGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCAt                                                                                    <  1:920553/137‑1 (MQ=255)
                                                     aGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCAt                                                                                    >  2:920553/1‑137 (MQ=255)
                                                      gCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATgg                                                                                  >  1:346124/1‑138 (MQ=255)
                                                      gCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATgg                                                                                  <  2:346124/138‑1 (MQ=255)
                                                      gCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAAC‑‑GTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAaga                                                                              >  2:608196/1‑140 (MQ=255)
                                                       ccATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAag                                                                               <  2:901979/140‑1 (MQ=255)
                                                          tGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAgg                                                                            <  1:1609811/140‑1 (MQ=255)
                                                          tGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAgg                                                                            >  2:305260/1‑140 (MQ=255)
                                                          tGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAgg                                                                            >  2:1167199/1‑140 (MQ=255)
                                                           gCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGgt                                                                           >  2:71951/1‑140 (MQ=255)
                                                           gCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGgt                                                                           >  2:1215040/1‑140 (MQ=255)
                                                             tATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTATATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGgtag                                                                         >  1:176782/1‑140 (MQ=255)
                                                              aTCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGgtagt                                                                        <  1:1690299/140‑1 (MQ=255)
                                                                 aGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcg                                                                     <  2:100018/140‑1 (MQ=255)
                                                                 aGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcg                                                                     <  1:923857/140‑1 (MQ=255)
                                                                 aGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcg                                                                     <  2:1534255/140‑1 (MQ=255)
                                                                 aGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcg                                                                     <  2:328692/140‑1 (MQ=255)
                                                                   cACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcgcg                                                                   <  2:1809863/140‑1 (MQ=255)
                                                                   cACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcgcg                                                                   <  1:1167199/140‑1 (MQ=255)
                                                                   cACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcgcg                                                                   <  1:71951/140‑1 (MQ=255)
                                                                    aCCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcg                                                                     <  2:433517/137‑1 (MQ=255)
                                                                    aCCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTAcg                                                                     >  1:433517/1‑137 (MQ=255)
                                                                      cGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCtt                                                                >  1:1450346/1‑140 (MQ=255)
                                                                      cGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCtt                                                                >  1:1042662/1‑140 (MQ=255)
                                                                       gCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCttt                                                               <  1:250324/140‑1 (MQ=255)
                                                                        ccAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTa                                                              <  1:1277991/140‑1 (MQ=255)
                                                                        ccAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTa                                                              <  2:403988/140‑1 (MQ=255)
                                                                          aGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACa                                                            <  1:1490208/140‑1 (MQ=255)
                                                                           gcggcgTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAg                                                           >  1:1227743/1‑140 (MQ=255)
                                                                              gcgTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAg                                                           >  2:1179873/1‑137 (MQ=255)
                                                                                gTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGaa                                                      <  2:1395297/140‑1 (MQ=255)
                                                                                gTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGaa                                                      <  1:1042891/140‑1 (MQ=255)
                                                                                gTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGaa                                                      <  1:283550/140‑1 (MQ=255)
                                                                                   ggCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATgg                                                   <  1:1837038/140‑1 (MQ=255)
                                                                                    gCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATggg                                                  <  2:1866974/140‑1 (MQ=255)
                                                                                     cGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGa                                                 <  1:305260/140‑1 (MQ=255)
                                                                                        aCATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACAt                                              <  2:732978/140‑1 (MQ=255)
                                                                                        aCATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACAt                                              <  2:1877383/140‑1 (MQ=255)
                                                                                                  gATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtgg                                    <  1:123170/140‑1 (MQ=255)
                                                                                                  gATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtgg                                    <  1:1215040/140‑1 (MQ=255)
                                                                                                    tAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtggct                                  >  2:1644450/1‑140 (MQ=255)
                                                                                                           gCGTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGaa                           >  1:551763/1‑140 (MQ=255)
                                                                                                             gTTAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACa                         >  2:1664854/1‑140 (MQ=255)
                                                                                                              ttAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACAt                        <  2:1042662/140‑1 (MQ=255)
                                                                                                               tAATTACGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGTGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACAtt                       <  1:1271719/140‑1 (MQ=255)
                                                                                                                    aCGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                  <  2:665584/140‑1 (MQ=255)
                                                                                                                    aCGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                  <  1:1145684/140‑1 (MQ=255)
                                                                                                                    aCGCGTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                  <  2:1425061/140‑1 (MQ=255)
                                                                                                                      gcgTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCaa                >  1:1486529/1‑140 (MQ=255)
                                                                                                                       cgTCTTAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCaaa               >  1:787924/1‑140 (MQ=255)
                                                                                                                           ttAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatt           >  1:1059742/1‑140 (MQ=255)
                                                                                                                            tAAATTACTGATTTCTGAGCGACAGGTAATTTCGATTTTAGTTTAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatta          >  2:1720961/1‑140 (MQ=255)
                                                                                                                            tAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatta          >  2:1811091/1‑140 (MQ=255)
                                                                                                                            tAAATTACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCACCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatta          >  1:1520142/1‑140 (MQ=255)
                                                                                                                                 tACTGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCg     <  2:551763/140‑1 (MQ=255)
                                                                                                                                    tGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCt  >  2:1561883/1‑140 (MQ=255)
                                                                                                                                    tGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCt  >  2:1597945/1‑140 (MQ=255)
                                                                                                                                    tGATTTCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCt  >  1:46773/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GCGAGGCGGCAAAAGCTGCTGGTATTGACCGCGTGTTAAGCGTGGGTAAACAAAGCCATGCTATCAGCACCGCCAGCGGCGTTGGCGAACATTTTGCTGATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCT  >  NC_000913/95776‑96047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑