Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,928,161	C→A	W481C (TGG→TGT)	viaA ←	protein ViaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,928,161	0	C	A	97.2%	247.3 / 0.6	72	W481C (TGG→TGT)	viaA	protein ViaA
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (36/34);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

GGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTT  >  NC_000913/3928028‑3928297                                                                                                                                      |                                                                                                                                         ggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGt                                                                                                                                    <  1:398702/140‑1 (MQ=255)     gcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGa                                                                                                                                >  2:1642068/1‑140 (MQ=255)       ggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGc                                                                                                                              >  2:533781/1‑140 (MQ=255)       ggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGc                                                                                                                              >  2:752456/1‑140 (MQ=255)             ggCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAggcgg                                                                                                                        <  1:131677/140‑1 (MQ=255)                  gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCg                                                                                                                   <  2:661707/140‑1 (MQ=255)                   tctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGc                                                                                                                  >  2:1207407/1‑140 (MQ=255)                     tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCAt                                                                                                                <  2:450464/140‑1 (MQ=255)                     tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCAt                                                                                                                <  2:158771/140‑1 (MQ=255)                     tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCAt                                                                                                                <  1:1569212/140‑1 (MQ=255)                     tcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCAt                                                                                                                <  1:533781/140‑1 (MQ=255)                       gTTTGACTATGCTGCTGCTGTAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATcc                                                                                                              <  2:507733/140‑1 (MQ=255)                             cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt                                                                                                        >  1:107215/1‑140 (MQ=255)                             cTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt                                                                                                        >  2:1358084/1‑140 (MQ=255)                               aTGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa                                                                                                      <  2:1745205/140‑1 (MQ=255)                                tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa                                                                                                     <  2:834806/140‑1 (MQ=255)                                tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa                                                                                                     <  2:1728922/140‑1 (MQ=255)                                 gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaaa                                                                                                    <  2:1681997/140‑1 (MQ=255)                                    gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg                                                                                                 <  1:1302701/140‑1 (MQ=255)                                    gctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg                                                                                                 <  1:1076199/140‑1 (MQ=255)                                     ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGc                                                                                                                  <  2:591009/122‑1 (MQ=255)                                     ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGc                                                                                                                  >  1:591009/1‑122 (MQ=255)                                     ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc                                                                                                >  1:872719/1‑140 (MQ=255)                                      tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGcc                                                                                               >  2:1076182/1‑140 (MQ=255)                                        ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAg                                                                                             <  2:107215/140‑1 (MQ=255)                                          gcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGat                                                                                           >  1:1639045/1‑140 (MQ=255)                                               tGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGAt                                                                                      >  1:1009993/1‑140 (MQ=255)                                                           gCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCa                                                                          <  1:355540/140‑1 (MQ=255)                                                               ggTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCatgat                                                                      <  2:1836696/140‑1 (MQ=255)                                                                 ttCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGc                                                                    <  2:491023/140‑1 (MQ=255)                                                                     gtgtGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCggg                                                                <  1:752456/140‑1 (MQ=255)                                                                          gAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGATTGc                                                           >  2:1185503/1‑140 (MQ=255)                                                                           aGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGcc                                                          <  1:1642068/140‑1 (MQ=255)                                                                                ggCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGtgtg                                                     >  2:1362369/1‑140 (MQ=255)                                                                                ggCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGtgtg                                                     >  2:29414/1‑140 (MQ=255)                                                                                ggCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGtgtg                                                     >  2:588351/1‑140 (MQ=255)                                                                                ggCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGtgtg                                                     >  2:952219/1‑140 (MQ=255)                                                                                  ccAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGcc                                                   >  1:963687/1‑140 (MQ=255)                                                                                  ccAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGcc                                                   >  1:681375/1‑140 (MQ=255)                                                                                  ccAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGcc                                                   >  2:1515218/1‑140 (MQ=255)                                                                                    aGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGa                                                 >  2:260688/1‑140 (MQ=255)                                                                                          tgTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGc                                           <  1:1362369/140‑1 (MQ=255)                                                                                          tgTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGc                                           <  1:1607742/140‑1 (MQ=255)                                                                                           gTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGcc                                          >  1:1191934/1‑140 (MQ=255)                                                                                            ttCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCa                                         <  1:588351/140‑1 (MQ=255)                                                                                            ttCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCa                                         <  2:1639045/140‑1 (MQ=255)                                                                                            ttCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCa                                         <  2:1410168/140‑1 (MQ=255)                                                                                                      cGTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGaaa                               >  2:1336486/1‑140 (MQ=255)                                                                                                       gTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAg                              <  1:741207/140‑1 (MQ=255)                                                                                                       gTCCCTTCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAg                              <  2:681375/140‑1 (MQ=255)                                                                                                            ttCTCTGCTGTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATg                         <  1:29414/140‑1 (MQ=255)                                                                                                                 tgctgTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGAt                    >  2:1396758/1‑140 (MQ=255)                                                                                                                   ctgTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGt                  >  1:1163482/1‑140 (MQ=255)                                                                                                                   ctgTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGt                  >  2:66133/1‑140 (MQ=255)                                                                                                                   ctgTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGt                  >  1:1731746/1‑140 (MQ=255)                                                                                                                   ctgTAATAATTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGt                  >  1:1210622/1‑140 (MQ=255)                                                                                                                      taataaTTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTAcc               >  1:863457/1‑140 (MQ=255)                                                                                                                        ataatTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGc                             <  1:703586/124‑1 (MQ=255)                                                                                                                        ataatTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGc                             >  2:703586/1‑124 (MQ=255)                                                                                                                        ataatTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCg             >  1:1690260/1‑140 (MQ=255)                                                                                                                           atTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTg          >  1:1525949/1‑140 (MQ=255)                                                                                                                           atTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTg          >  1:4802/1‑140 (MQ=255)                                                                                                                           atTTATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTg          >  1:895502/1‑140 (MQ=255)                                                                                                                            tttATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGc         <  1:952219/140‑1 (MQ=255)                                                                                                                             ttATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCa        >  1:1071483/1‑140 (MQ=255)                                                                                                                             ttATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCa        >  2:544476/1‑140 (MQ=255)                                                                                                                             ttATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCa        >  1:1637711/1‑140 (MQ=255)                                                                                                                              tATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAg       <  2:1009993/140‑1 (MQ=255)                                                                                                                              tATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAg       <  2:1731746/140‑1 (MQ=255)                                                                                                                              tATCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAg       <  2:1210622/140‑1 (MQ=255)                                                                                                                                tCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGct     >  2:899987/1‑140 (MQ=255)                                                                                                                                tCGCCGACAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGct     >  2:367438/1‑140 (MQ=255)                                                                                                                                   ccgacAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCtt  >  1:1583955/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         GGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTT  >  NC_000913/3928028‑3928297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑