BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	372,369	C→A	D85E (GAC→GAA)	mhpD →	2‑hydroxypentadienoate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,369	0	C	A	100.0%	416.7 / NA	114	D85E (GAC→GAA)	mhpD	2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (58/56); total (58/56)

Only 100 of 116 total aligned reads displayed.

TTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGTATAACGCCA  >  NC_000913/372236‑372502

ttCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATgtgt                                                                                                                                 >  2:832831/1‑140 (MQ=255)

 tCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGtt                                                                                                                                >  1:827218/1‑140 (MQ=255)

 tCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGtt                                                                                                                                <  2:1549169/140‑1 (MQ=255)

 tCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGtt                                                                                                                                >  2:1945074/1‑140 (MQ=255)

     cacaTAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATgg                                                                                                                            >  1:1106982/1‑140 (MQ=255)

       caTAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGTCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCg                                                                                                                          <  1:1776377/140‑1 (MQ=255)

        aTAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACGAGATTTTGATGCGTTATTTGCCGAAATGTGTTATGGCGa                                                                                                                         >  2:1691983/1‑140 (MQ=255)

        aTAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGa                                                                                                                         >  2:610151/1‑140 (MQ=255)

          aaaTGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCg                                                                                                                          <  1:1323524/137‑1 (MQ=255)

          aaaTGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCg                                                                                                                          >  2:1323524/1‑137 (MQ=255)

          aaaTGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATa                                                                                                                       >  2:651334/1‑140 (MQ=255)

             tgtgCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACg                                                                                                                    >  1:1696811/1‑140 (MQ=255)

               tgCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGaa                                                                                                                  >  2:1047149/1‑140 (MQ=255)

               tgCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGaa                                                                                                                  >  2:1595221/1‑140 (MQ=255)

                  aaCATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGAGGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATc                                                                                                               >  2:1898029/1‑140 (MQ=255)

                  aaCATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATc                                                                                                               >  1:1802932/1‑140 (MQ=255)

                   aCATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCa                                                                                                              <  1:651334/140‑1 (MQ=255)

                    cATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCAt                                                                                                             >  2:1954562/1‑140 (MQ=255)

                    cATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCAt                                                                                                             >  1:1055721/1‑140 (MQ=255)

                         cGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCAtt                                                                                                            >  2:1833194/1‑136 (MQ=255)

                         cGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCAtt                                                                                                            <  1:1833194/136‑1 (MQ=255)

                             gcgcAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTc                                                                                                    <  1:1047149/140‑1 (MQ=255)

                             gcgcAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTc                                                                                                    <  1:547547/140‑1 (MQ=255)

                               gcAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTccc                                                                                                  <  1:942545/140‑1 (MQ=255)

                                cAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCg                                                                                                 <  2:1294720/140‑1 (MQ=255)

                                  ggggCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCgtg                                                                                               <  1:1692403/140‑1 (MQ=255)

                                  ggggCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCgtg                                                                                               <  1:452181/140‑1 (MQ=255)

                                  ggggCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCgtg                                                                                               <  2:1087494/140‑1 (MQ=255)

                                  ggggCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCgtg                                                                                               <  2:1106982/140‑1 (MQ=255)

                                  ggggCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCgtg                                                                                               <  2:769621/140‑1 (MQ=255)

                                    ggCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGtt                                                                                             <  1:1695976/140‑1 (MQ=255)

                                        tCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGc                                                                                         >  2:1917650/1‑140 (MQ=255)

                                        tCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGc                                                                                         >  2:1611908/1‑140 (MQ=255)

                                        tCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGc                                                                                         >  2:155882/1‑140 (MQ=255)

                                        tCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGc                                                                                         >  2:1031848/1‑140 (MQ=255)

                                        tCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGc                                                                                         >  2:272955/1‑140 (MQ=255)

                                          gcgTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCaa                                                                                       <  2:777607/140‑1 (MQ=255)

                                                 aGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCa                                                                                <  1:948161/140‑1 (MQ=255)

                                                   ggCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCAtt                                                                              <  1:1339116/140‑1 (MQ=255)

                                                        aaaGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGc                                                                         >  1:506914/1‑140 (MQ=255)

                                                          aGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCgg                                                                       <  2:1696811/140‑1 (MQ=255)

                                                          aGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCgg                                                                       >  1:742790/1‑140 (MQ=255)

                                                             gggCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGgaga                                                                    <  1:1611908/140‑1 (MQ=255)

                                                             gggCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGgaga                                                                    >  2:1202926/1‑140 (MQ=255)

                                                              ggCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGAt                                                                   <  2:1622073/140‑1 (MQ=255)

                                                              ggCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGAt                                                                   >  2:1236332/1‑140 (MQ=255)

                                                              ggCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGAt                                                                   >  2:1132638/1‑140 (MQ=255)

                                                              ggCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGAt                                                                   >  1:229601/1‑140 (MQ=255)

                                                               gCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGCCGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATc                                                                  >  2:1106851/1‑140 (MQ=255)

                                                               gCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATc                                                                  >  1:1525606/1‑140 (MQ=255)

                                                                ccTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCg                                                                 <  2:827218/140‑1 (MQ=255)

                                                                ccTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCg                                                                 <  2:506914/140‑1 (MQ=255)

                                                                 cTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGc                                                                >  1:1860084/1‑140 (MQ=255)

                                                                   gACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCAc                                                              <  1:610151/140‑1 (MQ=255)

                                                                   gACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCAc                                                              >  1:1531255/1‑140 (MQ=255)

                                                                   gACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCAc                                                              >  2:736984/1‑140 (MQ=255)

                                                                   gACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCAc                                                              <  2:1860084/140‑1 (MQ=255)

                                                                   gACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCAc                                                              <  2:307932/140‑1 (MQ=255)

                                                                             aaaGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGaa                                                    >  2:1392705/1‑140 (MQ=255)

                                                                              aaGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAAc                                                   >  2:452511/1‑140 (MQ=255)

                                                                                   caacaacaaCTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGa                                              >  1:1195342/1‑140 (MQ=255)

                                                                                           acTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCg                                      >  1:717157/1‑140 (MQ=255)

                                                                                           acTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCg                                      >  2:486695/1‑140 (MQ=255)

                                                                                           acTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCg                                      >  2:1644203/1‑140 (MQ=255)

                                                                                            cTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAACTGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGc                                     >  1:781188/1‑140 (MQ=255)

                                                                                             tGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCa                                    <  1:1945074/140‑1 (MQ=255)

                                                                                             tGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCa                                    <  2:1789468/140‑1 (MQ=255)

                                                                                              gggCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCAATTTGCCCGCaa                                   <  2:927179/140‑1 (MQ=255)

                                                                                                gCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCaa                                   >  2:898557/1‑138 (MQ=255)

                                                                                                gCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCaa                                   <  1:898557/138‑1 (MQ=255)

                                                                                                 cGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGGGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCg                                >  2:822741/1‑140 (MQ=255)

                                                                                                 cGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGAttt                                           >  1:1340912/1‑129 (MQ=255)

                                                                                                 cGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGAttt                                           <  2:1340912/129‑1 (MQ=255)

                                                                                                 cGTTGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCg                                >  1:488965/1‑140 (MQ=255)

                                                                                                    tGATCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGata                             >  1:1757509/1‑140 (MQ=255)

                                                                                                      aTCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATc                           >  2:967084/1‑140 (MQ=255)

                                                                                                      aTCAACCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATc                           >  1:791960/1‑140 (MQ=255)

                                                                                                          aCCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCt                       <  1:1031848/140‑1 (MQ=255)

                                                                                                          aCCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCt                       >  2:102740/1‑140 (MQ=255)

                                                                                                          aCCGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCt                       >  1:1687083/1‑140 (MQ=255)

                                                                                                           ccGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCaa                                   >  1:1320029/1‑127 (MQ=255)

                                                                                                           ccGGATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCaa                                   <  2:1320029/127‑1 (MQ=255)

                                                                                                             ggATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcg                    <  2:177846/140‑1 (MQ=255)

                                                                                                             ggATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcg                    <  1:1132638/140‑1 (MQ=255)

                                                                                                             ggATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcg                    <  1:14579/140‑1 (MQ=255)

                                                                                                             ggATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcg                    <  2:1055721/140‑1 (MQ=255)

                                                                                                             ggATTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcg                    <  2:1687083/140‑1 (MQ=255)

                                                                                                               aTTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcgac                  <  2:488965/140‑1 (MQ=255)

                                                                                                               aTTTTGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcgac                  <  1:155882/140‑1 (MQ=255)

                                                                                                                ttttGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTcgacg                 <  1:981837/140‑1 (MQ=255)

                                                                                                                  ttGGGACGTTATTTGCCGAAATGTGTTATGGGGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGaa               <  1:822741/140‑1 (MQ=255)

                                                                                                                  ttGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGaa               <  2:1012109/140‑1 (MQ=255)

                                                                                                                  ttGGGACGTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGaa               <  1:1033675/140‑1 (MQ=255)

                                                                                                                         gTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGtata        >  2:564536/1‑140 (MQ=255)

                                                                                                                         gTTATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGtata        <  2:1757509/140‑1 (MQ=255)

                                                                                                                          ttATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGTATaa       <  1:1898029/140‑1 (MQ=255)

                                                                                                                          ttATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGTATaa       <  1:1392705/140‑1 (MQ=255)

                                                                                                                          ttATTTGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGTATaa       <  2:1802932/140‑1 (MQ=255)

                                                                                                                              ttGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGTATAACGcc   <  1:1595221/140‑1 (MQ=255)

                                                                                                                               tGCCGAAATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGTATAACGCCa  <  1:1644203/140‑1 (MQ=255)

TTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAAGTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGGACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTGCAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGATATCACCTTCGACGAATTGTATAACGCCA  >  NC_000913/372236‑372502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`