Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	415,246	C→A	A170S (GCG→TCG)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	415,246	0	C	A	100.0%	136.3 / NA	39	A170S (GCG→TCG)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/16);  total (23/16)

CCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTATCTTTCA  >  NC_000913/415108‑415368                                                                                                                                           |                                                                                                                           ccGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgcg                                                                                                                           <  2:309124/140‑1 (MQ=255)   ggCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgagcg                                                                                                                         >  2:1375967/1‑140 (MQ=255)    gCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGt                                                                                                                        >  2:854345/1‑140 (MQ=255)         aGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCttt                                                                                                                   >  2:1269610/1‑140 (MQ=255)                   cgccgcTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCa                                                                                                         >  2:586278/1‑140 (MQ=255)                              tGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGgc                                                                                              <  2:1560995/140‑1 (MQ=255)                                cgcTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGgcag                                                                                            >  1:386514/1‑140 (MQ=255)                                      cAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAAtt                                                                                      >  1:40484/1‑140 (MQ=255)                                           tctcCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGcccc                                                                                 >  1:1093766/1‑140 (MQ=255)                                           tctcCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGcccc                                                                                 >  2:782135/1‑140 (MQ=255)                                              ccAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGc                                                                              <  1:871436/140‑1 (MQ=255)                                                   tctGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAag                                                                         <  1:1422374/140‑1 (MQ=255)                                                       tgCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAagcagc                                                                     >  1:522259/1‑140 (MQ=255)                                                       tgCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAagcagc                                                                     >  1:706400/1‑140 (MQ=255)                                                       tgCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAagcagc                                                                     >  2:1743838/1‑140 (MQ=255)                                                       tgCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAagcagc                                                                     >  2:372152/1‑140 (MQ=255)                                                        gCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAgcagca                                                                    <  1:782135/140‑1 (MQ=255)                                                           ggCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCg                                                                 <  1:1796536/140‑1 (MQ=255)                                                           ggCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCg                                                                 <  2:1153377/140‑1 (MQ=255)                                                           ggCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAACAGCATCg                                                                 <  1:1269610/140‑1 (MQ=255)                                                           ggCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGACGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCg                                                                 <  2:889450/140‑1 (MQ=255)                                                             ccGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGaa                                                               <  2:442446/140‑1 (MQ=255)                                                             ccGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGaa                                                               <  2:40484/140‑1 (MQ=255)                                                                 tttGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACggg                                                           >  2:1034079/1‑140 (MQ=255)                                                                        tgctCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAgcg                                                    >  2:204613/1‑140 (MQ=255)                                                                          ctCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGcc                                                  >  1:810910/1‑140 (MQ=255)                                                                                    aTCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAg                                        >  2:1077850/1‑140 (MQ=255)                                                                                    aTCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAg                                        >  1:200313/1‑140 (MQ=255)                                                                                               ttGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACg                             <  2:386514/140‑1 (MQ=255)                                                                                                  cccGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCg                          >  1:569713/1‑140 (MQ=255)                                                                                                  cccGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCg                          <  1:739074/140‑1 (MQ=255)                                                                                                     gTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCACGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATGCAGCCCGGTTCACGTCGCTg                       <  2:57185/140‑1 (MQ=255)                                                                                                      tAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGt                      >  1:555546/1‑140 (MQ=255)                                                                                                      tAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGt                      <  1:1544636/140‑1 (MQ=255)                                                                                                      tAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGt                      <  2:706400/140‑1 (MQ=255)                                                                                                      tAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGt                      >  1:1389775/1‑140 (MQ=255)                                                                                                       aGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTc                     >  2:884656/1‑140 (MQ=255)                                                                                                            tCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCg                          <  2:1462039/130‑1 (MQ=255)                                                                                                            tCAGTGCCGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCg                          >  1:1462039/1‑130 (MQ=255)                                                                                                                  ccGGTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCtt          <  2:569713/140‑1 (MQ=255)                                                                                                                     gTTAACTCCTCGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTATc       >  2:1117607/1‑140 (MQ=255)                                                                                                                          ctcctcGAGCAATTCCGAGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTATCTTTCa  >  2:1544201/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                           CCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTATCTTTCA  >  NC_000913/415108‑415368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑