BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,678,604	G→T	A60S (GCA→TCA)	ydgH →	DUF1471 domain‑containing protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,678,604	0	G	T	100.0%	364.4 / NA	102	A60S (GCA→TCA)	ydgH	DUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (44/58); total (44/58)

Only 100 of 104 total aligned reads displayed.

TGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAAT  >  NC_000913/1678468‑1678737

tGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAg                                                                                                                                    <  1:297151/140‑1 (MQ=255)

    aTGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaa                                                                                                                                <  2:192777/140‑1 (MQ=255)

     tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaaa                                                                                                                               >  1:1390666/1‑140 (MQ=255)

     tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaaa                                                                                                                               >  2:420609/1‑140 (MQ=255)

       gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaaga                                                                                                                             >  2:285670/1‑140 (MQ=255)

          ttCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAgg                                                                                                                          >  2:1125808/1‑140 (MQ=255)

            ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                        >  1:1255249/1‑140 (MQ=255)

            ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                        >  2:317477/1‑140 (MQ=255)

            ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                        >  2:830457/1‑140 (MQ=255)

             tcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgc                                                                                                                       >  1:340556/1‑140 (MQ=255)

              ccGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgcc                                                                                                                      <  1:1111510/140‑1 (MQ=255)

               cGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccg                                                                                                                     <  1:1125808/140‑1 (MQ=255)

                gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgtcgc                                                                                                                    <  1:450505/140‑1 (MQ=255)

                gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgc                                                                                                                    <  1:1115042/140‑1 (MQ=255)

                gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgc                                                                                                                    <  1:285670/140‑1 (MQ=255)

                gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgc                                                                                                                    <  1:655445/140‑1 (MQ=255)

                gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgc                                                                                                                    <  1:441336/140‑1 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGCTAAAGAAGGTgccgcc                                                                                                                   <  2:1243054/140‑1 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                   >  2:500831/1‑140 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                   >  2:1060647/1‑140 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                   <  1:1227937/140‑1 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                   <  1:1136328/140‑1 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                   <  2:1718493/140‑1 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                   <  2:1775674/140‑1 (MQ=255)

                 ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGCCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                   >  1:457160/1‑140 (MQ=255)

                          caACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAt                                                                                                          <  2:340556/140‑1 (MQ=255)

                          caACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAt                                                                                                          >  1:56790/1‑140 (MQ=255)

                          caACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAt                                                                                                          >  1:1040055/1‑140 (MQ=255)

                           aaCAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtg                                                                                                         <  2:569106/140‑1 (MQ=255)

                             cAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtgtt                                                                                                       <  2:1432885/140‑1 (MQ=255)

                             cAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtgtt                                                                                                       <  2:1913848/140‑1 (MQ=255)

                               gAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtgttgt                                                                                                     <  2:192476/140‑1 (MQ=255)

                               gAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtgttgt                                                                                                     <  1:1402630/140‑1 (MQ=255)

                                  cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                  <  2:56790/140‑1 (MQ=255)

                                  cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                  <  1:1752592/140‑1 (MQ=255)

                                    gACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGaca                                                                                                >  1:129661/1‑140 (MQ=255)

                                     acacCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGacac                                                                                               >  1:764748/1‑140 (MQ=255)

                                      cacCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACt                                                                                              <  1:317477/140‑1 (MQ=255)

                                       acCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACtt                                                                                             >  2:809896/1‑140 (MQ=255)

                                          ggAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                          >  2:1164775/1‑140 (MQ=255)

                                            aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                  <  2:1436446/130‑1 (MQ=255)

                                            aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                  >  1:1436446/1‑130 (MQ=255)

                                            aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAt                                                                                        >  2:78496/1‑140 (MQ=255)

                                            aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAt                                                                                        >  1:1551153/1‑140 (MQ=255)

                                            aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAt                                                                                        <  2:793446/140‑1 (MQ=255)

                                            aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAt                                                                                        >  2:1452288/1‑140 (MQ=255)

                                                  cGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGt                                                                                  <  2:1473577/140‑1 (MQ=255)

                                                    gCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTaa                                                                                <  1:830457/140‑1 (MQ=255)

                                                      aGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACa                                                                              <  1:860537/140‑1 (MQ=255)

                                                      aGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACa                                                                              <  2:1084174/140‑1 (MQ=255)

                                                          gTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCgg                                                                          <  2:1713107/140‑1 (MQ=255)

                                                                    ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                <  1:582655/140‑1 (MQ=255)

                                                                    ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                >  2:1773608/1‑140 (MQ=255)

                                                                     tGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACa                                                                              <  2:252924/125‑1 (MQ=255)

                                                                           tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                         <  1:120907/140‑1 (MQ=255)

                                                                            gtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGc                                                        <  1:809896/140‑1 (MQ=255)

                                                                             tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGc                                                        >  2:282949/1‑139 (MQ=255)

                                                                             tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGc                                                        <  1:282949/139‑1 (MQ=255)

                                                                             tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                       <  2:764748/140‑1 (MQ=255)

                                                                             tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                       <  1:665839/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  2:1044219/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  2:1503206/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  2:1568052/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  1:1269885/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  2:1203573/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  1:1329476/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  1:292174/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  1:81399/140‑1 (MQ=255)

                                                                                tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  1:500831/140‑1 (MQ=255)

                                                                                 ggTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAcc                                                   <  1:926437/140‑1 (MQ=255)

                                                                                  gTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACct                                                  >  2:303189/1‑140 (MQ=255)

                                                                                     aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    >  1:58294/1‑135 (MQ=255)

                                                                                     aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                    <  2:58294/135‑1 (MQ=255)

                                                                                     aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                               >  2:923530/1‑140 (MQ=255)

                                                                                     aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                               >  2:1866635/1‑140 (MQ=255)

                                                                                          tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                          >  1:1737418/1‑140 (MQ=255)

                                                                                            gTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGcc                                        >  1:692360/1‑140 (MQ=255)

                                                                                             ttttAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCg                                       >  2:1365938/1‑140 (MQ=255)

                                                                                                       aTTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaa                             <  1:1833874/140‑1 (MQ=255)

                                                                                                        ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                            >  1:1313076/1‑140 (MQ=255)

                                                                                                        ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                            >  1:1768537/1‑140 (MQ=255)

                                                                                                        ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                            >  2:1098199/1‑140 (MQ=255)

                                                                                                        ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                            >  2:511046/1‑140 (MQ=255)

                                                                                                        ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                            >  1:333792/1‑140 (MQ=255)

                                                                                                        ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTAAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                            >  1:52996/1‑140 (MQ=255)

                                                                                                         tGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAg                           <  2:333792/140‑1 (MQ=255)

                                                                                                          ggCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGTCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGc                          >  1:861700/1‑140 (MQ=255)

                                                                                                           gCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGTCCTCTATAAAGCCGATGCTGAAAAAGCa                         <  2:457160/140‑1 (MQ=255)

                                                                                                               aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                     <  2:1630280/140‑1 (MQ=255)

                                                                                                               aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                     >  2:179007/1‑140 (MQ=255)

                                                                                                                        aGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTa            <  1:179007/140‑1 (MQ=255)

                                                                                                                             tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc       <  1:420609/140‑1 (MQ=255)

                                                                                                                             tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc       >  2:1186588/1‑140 (MQ=255)

                                                                                                                             tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc       >  1:1055649/1‑140 (MQ=255)

                                                                                                                              ttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgcg      <  2:1255249/140‑1 (MQ=255)

                                                                                                                              ttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgcg      >  1:1415114/1‑140 (MQ=255)

                                                                                                                               tctcGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGt     <  2:1313076/140‑1 (MQ=255)

                                                                                                                               tctcGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGt     <  1:1060647/140‑1 (MQ=255)

                                                                                                                               tctcGTCGCTCAGATAAAGAAGGTGCCGCCTCATTTTATGTTGTCGACACTTCTGATCTTGGCAACAGCTGTAACTGGCGAGTGGTCACTAACCTCTATAAAGCCGATGCTCAAAAAGCAGAAGAAACAAGTAATCCCGt     <  2:902558/140‑1 (MQ=255)

                                                                                                                                  cgtcgCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAAt  >  1:1078647/1‑140 (MQ=255)

TGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAAT  >  NC_000913/1678468‑1678737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`