| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,809 | 0 | T | G | 32.6% | 88.9 / 19.3 | 49 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (9/24); new base G (16/0); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.30e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATA > NC_000913/650677‑650946 | ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTTATTTTTCACCTGTTTC‑TCTTGCGGGGGGCTTTTGa > 2:381928/1‑140 (MQ=255)ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACAACCCCAGCCTCAAGGGTGCCTGCAACGACGGACTGGTTTTTTTTTATTTTTCTGCTAATTCTTCAGGCGGGGTGCttttt > 2:1170413/1‑139 (MQ=255) ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTCACCTGTTTT‑TCATGCGGGGGGCTTTTTACGa > 2:694206/1‑140 (MQ=255) aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGcc < 2:795325/140‑1 (MQ=255) gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGt < 1:621502/140‑1 (MQ=255) cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTc > 1:276483/1‑140 (MQ=255) cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTc > 1:1307513/1‑140 (MQ=255) cAGGTCGATATCCTGCGAATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCTGGCTCGTTTTTTTTCTCGTTTTACCCGGTTC‑TCGTGCGGGGGGTTTTTGATGGACCGCCGCGTc > 2:1119210/1‑140 (MQ=255) cGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGc < 1:1585893/140‑1 (MQ=255) cGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTCTTTTTCCACTGTTTC‑TCATACGGGGGGCTTTTGACGAGCTGCCGCGTTCTGGc > 2:1446379/1‑140 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGc > 1:1599639/1‑140 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTACCTGTTTT‑TCCTGCGGGGGGCTTTTTACGGGCTGCCGCGTCCTggggggggggc > 2:336863/1‑135 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTACCTGTTTT‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCgggggggc > 2:1585893/1‑135 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTc > 1:591137/1‑140 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGGTc > 1:641112/1‑140 (MQ=255) tATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCaa > 1:1239106/1‑140 (MQ=255) tCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc < 1:381928/140‑1 (MQ=255) cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCa > 1:1411843/1‑140 (MQ=255) cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTTACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAGCa > 2:1257248/1‑140 (MQ=255) gAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAg < 2:314790/140‑1 (MQ=255) aGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTTCCTGTTTC‑TCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCaggagg > 2:539523/1‑140 (MQ=255) ggggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTT‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAGCAgggtt > 1:1554393/1‑137 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt < 1:539523/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt < 2:1599639/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt < 1:1257248/140‑1 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTCATTTTTTACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGttttt > 2:658141/1‑137 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTT‑TCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCAGGGTCTg > 2:834949/1‑140 (MQ=255) cGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAaataa < 2:1447312/140‑1 (MQ=255) tATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATg < 2:519301/140‑1 (MQ=255) atcatcACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGc < 1:795707/140‑1 (MQ=255) catcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAg < 2:1693358/140‑1 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGTTCTGCAAATAATGCAGCgg > 1:885704/1‑139 (MQ=255) tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTCTGCCAATAAAGCAGCggggct > 2:1057245/1‑135 (MQ=255) tCCCCAGACTCAAGGGGGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGGTCTGCAAAAAATGCAGCGTGGCt > 2:1498647/1‑140 (MQ=255) tCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGAcc < 1:1057245/140‑1 (MQ=255) aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAg < 2:1411843/140‑1 (MQ=255) aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAACAGGGTCTGCAAAAAATGCAGCGTGACTGCAGGGGCCAg > 1:936601/1‑140 (MQ=255) aGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgc < 1:117847/140‑1 (MQ=255) tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggcg < 1:1498647/140‑1 (MQ=255) tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGTTTTGCAAAAAATGCAGCGGGACTGCCGGGGCCAgcgggg > 2:670967/1‑138 (MQ=255) gCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGGTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGgcgc > 1:257438/1‑140 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACAGGGTCTGCAAATAAAGCAGCGTGACTGCAGGGACCAGCGGcgcg > 1:471328/1‑140 (MQ=255) ccTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGGAGGGTTTTCCAAAAAAGGAGCGGGAATGCCGGGGCCAGCGGggcg > 2:1107474/1‑140 (MQ=255) cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGa < 1:1054306/140‑1 (MQ=255) tGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGAt < 1:1119210/140‑1 (MQ=255) cAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAACAGGTTTTGCAAAAAATTCAGCGTGACTGCAGGGACCAGCGGCGCGATAg > 2:446875/1‑140 (MQ=255) ggcggGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGt < 2:591137/140‑1 (MQ=255) aTTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc > 2:1040628/1‑140 (MQ=255) cACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGa > 2:1095733/1‑140 (MQ=255) tttC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc > 1:661495/1‑140 (MQ=255) aTGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGaa < 2:782743/140‑1 (MQ=255) tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg < 1:670967/140‑1 (MQ=255) cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg > 2:831286/1‑140 (MQ=255) cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg < 2:936601/140‑1 (MQ=255) gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg > 1:1472405/1‑140 (MQ=255) gggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGat < 1:834949/140‑1 (MQ=255) ggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGata < 2:881174/140‑1 (MQ=255) | CTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTC‑TCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATA > NC_000913/650677‑650946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |