Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,067,274	T→C	Q16Q (CAA→CAG)	insH‑6 ←	CP4‑44 prophage; IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,067,274	0	T	C	100.0%	202.2 / NA	67	Q16Q (CAA→CAG)	insH‑6	CP4‑44 prophage; IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/38);  total (29/38)

CGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCAA  >  NC_000913/2067162‑2067404                                                                                                                 |                                                                                                                                   cGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCa                                                                                                         <  2:362690/140‑1 (MQ=14)  gATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCAt                                                                                                        <  2:1136385/140‑1 (MQ=17)   aTGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATg                                                                                                       >  1:989388/1‑140 (MQ=17)   aTGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATg                                                                                                       >  1:1482056/1‑140 (MQ=17)    tGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATgg                                                                                                      >  2:693601/1‑140 (MQ=17)        ttCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTcc                                                                                                  <  1:869784/140‑1 (MQ=18)              caTGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGa                                                                                            >  1:684334/1‑140 (MQ=17)               aTGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGAc                                                                                           <  1:1090379/140‑1 (MQ=18)                  ttttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACaaa                                                                                        >  2:387846/1‑140 (MQ=17)                   tttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAAc                                                                                       <  2:1482056/140‑1 (MQ=18)                    ttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACa                                                                                      <  2:974637/140‑1 (MQ=18)                    ttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACa                                                                                      <  1:67647/140‑1 (MQ=17)                    ttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACa                                                                                      <  1:568482/140‑1 (MQ=18)                    ttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACa                                                                                      <  2:599345/140‑1 (MQ=18)                    ttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACa                                                                                      <  1:1455199/140‑1 (MQ=17)                     tGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACAt                                                                                     <  1:154218/140‑1 (MQ=17)                     tGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACAt                                                                                     <  2:375359/140‑1 (MQ=17)                     tGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACAt                                                                                     <  2:780405/140‑1 (MQ=17)                          tGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGAtct                                                                                >  2:1560432/1‑140 (MQ=17)                          tGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGAtct                                                                                >  1:404522/1‑140 (MQ=17)                          tGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGAtct                                                                                >  1:1291127/1‑140 (MQ=17)                            gCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCa                                                                              <  2:684334/140‑1 (MQ=17)                            gCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCa                                                                              >  1:1311053/1‑140 (MQ=17)                            gCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCa                                                                              >  1:965560/1‑140 (MQ=17)                                    tGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAggg                                                                      >  2:1596465/1‑140 (MQ=17)                                     gCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGa                                                                     <  1:408341/140‑1 (MQ=18)                                      cTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGAc                                                                    <  1:24048/140‑1 (MQ=18)                                      cTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGAc                                                                    >  1:1177470/1‑140 (MQ=17)                                       tCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACt                                                                   >  1:96261/1‑140 (MQ=17)                                           tGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGtt                                                               >  1:884905/1‑140 (MQ=17)                                           tGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGtt                                                               >  1:1467232/1‑140 (MQ=17)                                              gggACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGc                                                            <  2:1291127/140‑1 (MQ=18)                                                 aCAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAcc                                                         <  2:96261/140‑1 (MQ=17)                                                 aCAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAcc                                                         <  2:760023/140‑1 (MQ=18)                                                 aCAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAcc                                                         <  2:378804/140‑1 (MQ=18)                                                   aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCtt                                                       >  2:243558/1‑140 (MQ=17)                                                     gAAAATCTCTTTTCTGGCCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTcc                                                     >  1:1501246/1‑140 (MQ=17)                                                           ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGaa                                               <  1:243558/140‑1 (MQ=37)                                                             ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAaca                                             >  2:1523739/1‑140 (MQ=38)                                                               tttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcacc                                           >  1:1089123/1‑140 (MQ=255)                                                                ttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcacca                                          >  2:1423179/1‑140 (MQ=255)                                                                ttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcacca                                          >  2:406788/1‑140 (MQ=255)                                                                     gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAAtt                                     >  1:1064036/1‑140 (MQ=255)                                                                     gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAAtt                                     >  2:1396383/1‑140 (MQ=255)                                                                     gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAAtt                                     >  2:218763/1‑140 (MQ=255)                                                                       cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTc                                   <  1:1396383/140‑1 (MQ=255)                                                                       cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTc                                   <  1:1596888/140‑1 (MQ=255)                                                                        tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCg                                  >  1:175616/1‑140 (MQ=255)                                                                        tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCg                                  <  1:952960/140‑1 (MQ=255)                                                                        tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCg                                  <  1:1596465/140‑1 (MQ=255)                                                                          aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGct                                >  1:467206/1‑140 (MQ=255)                                                                          aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGct                                >  1:1179775/1‑140 (MQ=255)                                                                            ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctct                              <  1:569526/140‑1 (MQ=255)                                                                            ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctct                              <  2:1179775/140‑1 (MQ=255)                                                                              cgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctctct                            <  1:387846/140‑1 (MQ=255)                                                                               gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCtctctc                           >  2:850896/1‑140 (MQ=255)                                                                                 ttACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCgg                         <  1:1560432/140‑1 (MQ=255)                                                                                  tACTGCTGAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGt                        <  2:1064036/140‑1 (MQ=255)                                                                                      gctgAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAgg                    <  2:1311053/140‑1 (MQ=255)                                                                                       ctgAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGc                   >  2:193900/1‑140 (MQ=255)                                                                                        tgAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCa                  <  1:218763/140‑1 (MQ=255)                                                                                         gAATTCACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCAc                 <  1:892947/140‑1 (MQ=255)                                                                                              cACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTca            <  2:539229/140‑1 (MQ=255)                                                                                              cACTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTca            <  2:965560/140‑1 (MQ=255)                                                                                                cTGTCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTcaca          <  1:693601/140‑1 (MQ=255)                                                                                                   tCGGCGAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCa       <  2:1177470/140‑1 (MQ=255)                                                                                                        gAAGGTAAGCTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCaa  <  2:378206/140‑1 (MQ=255)                                                                                                                 |                                                                                                                                   CGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCACAGCATTCAA  >  NC_000913/2067162‑2067404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑