Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,598,092	C→A	A258A (GCG→GCT)	bamC ←	outer membrane protein assembly factor BamC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,598,092	0	C	A	100.0%	254.5 / NA	70	A258A (GCG→GCT)	bamC	outer membrane protein assembly factor BamC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/33);  total (37/33)

TATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGG  >  NC_000913/2597956‑2598228                                                                                                                                         |                                                                                                                                         tataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGagcc                                                                                                                                       <  1:633636/140‑1 (MQ=255)       cACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGc                                                                                                                                 >  2:769502/1‑140 (MQ=255)         ccGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAg                                                                                                                               >  2:1445803/1‑140 (MQ=255)         ccGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAg                                                                                                                               >  2:1195066/1‑140 (MQ=255)           ggATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGAc                                                                                                                             <  1:783793/140‑1 (MQ=255)             aTGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGt                                                                                                                           >  2:457307/1‑140 (MQ=255)                 cAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGcc                                                                                                                       >  1:763322/1‑140 (MQ=255)                 cAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGcc                                                                                                                       <  1:745660/140‑1 (MQ=255)                    gCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCaaa                                                                                                                    <  2:1216842/140‑1 (MQ=255)                         gATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACcaca                                                                                                               >  2:1283064/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTg                                                                                                                         <  2:804211/125‑1 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTg                                                                                                                         >  1:804211/1‑125 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCaaa                                                                                                                    >  1:567242/1‑130 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCaaa                                                                                                                    <  2:567242/130‑1 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:929330/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          <  1:979012/140‑1 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:606767/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  2:100481/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  2:1447783/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  2:1577720/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:442481/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:1679993/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  2:776504/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:1510042/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  2:84663/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  2:854016/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:1427541/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:1449472/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACAACATTGaa                                                                                                          >  2:352871/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGACAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:590256/1‑140 (MQ=255)                              ctcgcGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGCCACTTTCATGCCCACTTTTTCCAGAGCCTCTGGCAGACGTTGCCAAACCACATTGaa                                                                                                          >  1:1111138/1‑140 (MQ=255)                                 gcgcCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAGGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACgg                                                                                                       <  2:1636460/140‑1 (MQ=255)                                       aGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCcgcg                                                                                                 <  2:442481/140‑1 (MQ=255)                                             tGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGAc                                                                                           <  1:854016/140‑1 (MQ=255)                                             tGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGAc                                                                                           <  1:1577720/140‑1 (MQ=255)                                                cAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACTGCCCGCGTACGACCAg                                                                                        <  2:1073890/140‑1 (MQ=255)                                                cAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAg                                                                                        <  2:1607280/140‑1 (MQ=255)                                                cAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAg                                                                                        <  2:1427541/140‑1 (MQ=255)                                                 aGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGACAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGc                                                                                       <  2:590256/140‑1 (MQ=255)                                                  gTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCa                                                                                      >  2:966731/1‑140 (MQ=255)                                                   tCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCAt                                                                                     >  1:1455491/1‑140 (MQ=255)                                                   tCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCAt                                                                                     <  2:1679993/140‑1 (MQ=255)                                                      cTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTgg                                                                                  >  2:1483797/1‑140 (MQ=255)                                                                 cGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGgtgt                                                                       >  2:1510371/1‑140 (MQ=255)                                                                  ggCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGtc                                                                      <  1:100481/140‑1 (MQ=255)                                                                  ggCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGtc                                                                      <  1:1447622/140‑1 (MQ=255)                                                                  ggCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGtc                                                                      <  1:776504/140‑1 (MQ=255)                                                                   gCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGtca                                                                     >  1:1243606/1‑140 (MQ=255)                                                                    cTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGtcat                                                                    <  2:1510042/140‑1 (MQ=255)                                                                            ttACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCa                                                            <  1:1283064/140‑1 (MQ=255)                                                                            ttACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCa                                                            <  1:1447783/140‑1 (MQ=255)                                                                             tACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCAc                                                           <  1:966731/140‑1 (MQ=255)                                                                              aCGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAATCACATTGAACGGCCCGCGTACGACCAGCATTGGTATACCGGTGTCATCAGCTGAACt                                                          >  2:972199/1‑140 (MQ=255)                                                                              aCGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACt                                                          >  1:1669896/1‑140 (MQ=255)                                                                                 gCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTg                                                       <  2:625893/140‑1 (MQ=255)                                                                                  ccATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACAACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGt                                                      <  1:352871/140‑1 (MQ=255)                                                                                  caaTGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGt                                                      <  2:1270832/138‑1 (MQ=255)                                                                                    aTGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTAc                                                    <  2:1449472/140‑1 (MQ=255)                                                                                    aTGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTAc                                                    <  1:1496847/140‑1 (MQ=255)                                                                                     tGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACg                                                   <  1:84663/140‑1 (MQ=255)                                                                                     tGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACg                                                   <  1:1097807/140‑1 (MQ=255)                                                                                      gTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGt                                                  >  2:604440/1‑140 (MQ=255)                                                                                               cGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                         >  2:497479/1‑140 (MQ=255)                                                                                               cGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                         >  1:593975/1‑140 (MQ=255)                                                                                               cGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAgtgg                                         >  2:744531/1‑140 (MQ=255)                                                                                                       tGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCa                                 <  1:744531/140‑1 (MQ=255)                                                                                                         cTGTCGGTCACTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACg                               >  2:8189/1‑140 (MQ=255)                                                                                                                  aCTTTCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTgcgc                      <  2:1455491/140‑1 (MQ=255)                                                                                                                    tttCATGCCCACTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAg                    >  1:1611018/1‑140 (MQ=255)                                                                                                                              aCTTTTTCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTAGTGGAGGCACGATTTTGCGCAGCGTTCgcggc          >  1:1169412/1‑140 (MQ=255)                                                                                                                                tttttCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGt        >  1:557289/1‑140 (MQ=255)                                                                                                                                tttttCCAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGt        <  1:1483797/140‑1 (MQ=255)                                                                                                                                      cAGAGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTgg  <  2:1243606/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCCGCTGGCAGACGTTGCCAAACCACATTGAACGGCCCGCGTACGACCAGCATTGGTAAACCGGTGTCATCAGCTGCACTTTGTACGTCCATAGTGGTGGAGGCACGATTTTGCGCAGCGTTCGCGGCGTCAGTGG  >  NC_000913/2597956‑2598228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑