Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,053,387	G→T	G44G (GGC→GGA)	ubiH ←	2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,053,387	0	G	T	100.0%	171.5 / NA	50	G44G (GGC→GGA)	ubiH	2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/19);  total (31/19)

TCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTT  >  NC_000913/3053255‑3053523                                                                                                                                     |                                                                                                                                         tCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGAt                                                                                                                                   <  2:1650082/140‑1 (MQ=255) tCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGAt                                                                                                                                   <  1:734056/140‑1 (MQ=255)      cGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCa                                                                                                                              >  2:1447288/1‑140 (MQ=255)      cGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCa                                                                                                                              >  1:948512/1‑140 (MQ=255)         gACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCAtgt                                                                                                                           <  1:1447288/140‑1 (MQ=255)          aCCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCAtgtg                                                                                                                          >  1:101386/1‑140 (MQ=255)          aCCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCAtgtg                                                                                                                          >  1:1267952/1‑140 (MQ=255)          aCCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCAtgtg                                                                                                                          >  1:996239/1‑140 (MQ=255)          aCCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCAtgtg                                                                                                                          >  1:763555/1‑140 (MQ=255)           ccACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGa                                                                                                                         >  1:1119685/1‑140 (MQ=255)                      gACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGTTTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGa                                                                                                                         <  1:1566350/129‑1 (MQ=255)                      gACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGa                                                                                                                         >  2:1566350/1‑129 (MQ=255)                           gCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCGGACCCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCt                                                                                                         <  2:1628510/140‑1 (MQ=255)                           gCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCt                                                                                                         <  2:996239/140‑1 (MQ=255)                            cACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGt                                                                                                             >  1:830392/1‑135 (MQ=255)                            cACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGt                                                                                                             <  2:830392/135‑1 (MQ=255)                            cACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGc                                                                                                          <  1:1149367/138‑1 (MQ=255)                            cACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGc                                                                                                          >  2:1149367/1‑138 (MQ=255)                             aCGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTtc                                                                                                       >  1:993426/1‑140 (MQ=255)                               ggtggtGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTtcaa                                                                                                     >  2:1361667/1‑140 (MQ=255)                                gtggtgATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTtcaat                                                                                                    <  2:204025/140‑1 (MQ=255)                                 tggtgATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTtcaatc                                                                                                   >  1:776234/1‑140 (MQ=255)                                 tggtgATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTtcaatc                                                                                                   >  1:10241/1‑140 (MQ=255)                                 tggtgATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTtcaatc                                                                                                   >  2:1010952/1‑140 (MQ=255)                                 tggtgATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTtcaatc                                                                                                   >  1:467685/1‑140 (MQ=255)                                           aGTTGCGCAATCCGCCAGAGATTGCCATACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCg                                                                                         >  2:1021234/1‑140 (MQ=255)                                           aGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCg                                                                                         >  2:34421/1‑140 (MQ=255)                                           aGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCg                                                                                         <  2:1302793/140‑1 (MQ=255)                                             ttGCGCAATCCGCCAGAGCTTGCCAGACGCCGATGCGCGCCAGTTGATGACAGGCACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGc                                                                                       >  2:1212447/1‑140 (MQ=255)                                             ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGc                                                                                       >  2:1003303/1‑140 (MQ=255)                                             ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGc                                                                                       >  2:850936/1‑140 (MQ=255)                                             ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCCCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGc                                                                                       >  2:227787/1‑140 (MQ=255)                                                      ccGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCg                                                                              <  2:1379716/140‑1 (MQ=255)                                                                ttGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAAcc                                                                    <  2:10241/140‑1 (MQ=255)                                                                      gACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGaaa                                                              <  1:1010952/140‑1 (MQ=255)                                                                       aCGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAAt                                                             >  2:1371534/1‑140 (MQ=255)                                                                         gCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAg                                                           >  2:114261/1‑140 (MQ=255)                                                                             aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTCACCGGGAAATAgccag                                                       >  2:1023723/1‑140 (MQ=255)                                                                                cgcgcCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAgccagcgc                                                    <  2:948512/140‑1 (MQ=255)                                                                                  cgcCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAgccagcgcca                                                  >  1:673988/1‑140 (MQ=255)                                                                                     cAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGccagcgccagcg                                               >  1:157922/1‑140 (MQ=255)                                                                                     cAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGccagcgccagcg                                               <  1:1371534/140‑1 (MQ=255)                                                                                                     cccGCCGCCAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATgcc                               >  1:905749/1‑140 (MQ=255)                                                                                                          cgccAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCAc                          >  2:1187134/1‑140 (MQ=255)                                                                                                            ccAGCGCTATCGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACcg                        >  2:693472/1‑140 (MQ=255)                                                                                                                      cGCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTACCCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTa              <  2:467685/140‑1 (MQ=255)                                                                                                                       gCTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTACCCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTAc             <  2:1315356/140‑1 (MQ=255)                                                                                                                       gCTCGTCCAACAACCCCGGGAGGAGCAGGTGCCTTTGGGGCAGCCACTTCAAGCAAATGGACCGGCTGCGCCCCGTGACTTAGCCGGGAAATAGCCCGCGCCACCGTCGCGCCCGCCATGCCGCCACCGACGATGATTAc             <  2:993426/140‑1 (MQ=255)                                                                                                                        cTCGTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGAAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACg            <  2:776234/140‑1 (MQ=255)                                                                                                                           gTCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTc         >  1:655762/1‑140 (MQ=255)                                                                                                                            tCCATCAAATCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCa        >  1:933607/1‑140 (MQ=255)                                                                                                                                  aaaTCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCtt  <  2:1267952/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TCCAGCGTGACCACGATCGCTGACATGCACGGTGGTGATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTT  >  NC_000913/3053255‑3053523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑