| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,224,019 | G→T | A463S (GCG→TCG) | ebgA → | evolved beta‑D‑galactosidase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,224,019 | 0 | G | T | 99.1% | 404.0 / ‑3.5 | 113 | A463S (GCG→TCG) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (49/63); total (50/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
TCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCG > NC_000913/3223883‑3224155 | tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGa < 2:556775/140‑1 (MQ=255)tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGa < 1:400983/140‑1 (MQ=255)tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGa > 2:1092991/1‑140 (MQ=255) ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGaa < 1:571291/140‑1 (MQ=255) ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGaa < 1:513900/140‑1 (MQ=255) cGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGaaa > 2:639880/1‑140 (MQ=255) tGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCt > 1:185497/1‑140 (MQ=255) ggAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTgg > 2:1256606/1‑140 (MQ=255) gAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGa < 1:956676/140‑1 (MQ=255) gAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGa < 1:691830/140‑1 (MQ=255) gAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGa < 1:1579675/140‑1 (MQ=255) aaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATg > 1:894235/1‑140 (MQ=255) aaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATg > 1:186224/1‑140 (MQ=255) aaaGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGTAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGa < 1:775891/140‑1 (MQ=255) aGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGaca < 1:659376/140‑1 (MQ=255) cTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACAcgc < 2:12611/140‑1 (MQ=255) cTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACAcgc < 2:894235/140‑1 (MQ=255) tACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACAcgcg > 2:166956/1‑140 (MQ=255) aCGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGa < 1:438293/140‑1 (MQ=255) cGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGAc < 1:1533709/140‑1 (MQ=255) cGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGAc > 2:882083/1‑140 (MQ=255) gTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACt < 1:1573547/140‑1 (MQ=255) cgcATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCa < 2:717794/140‑1 (MQ=255) cATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCAtt < 2:1070361/140‑1 (MQ=255) gTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACga < 1:169393/140‑1 (MQ=255) atCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCGTGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg > 2:382354/1‑140 (MQ=255) atCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg > 1:775914/1‑140 (MQ=255) atCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg < 1:1503/140‑1 (MQ=255) atCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg > 1:694667/1‑140 (MQ=255) atCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg < 2:352297/140‑1 (MQ=255) ccACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg > 2:1609910/1‑140 (MQ=255) ccACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg > 2:237027/1‑140 (MQ=255) cgcgCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg > 2:1459880/1‑140 (MQ=255) cgcgCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg > 2:724480/1‑140 (MQ=255) cgcgCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg > 1:87344/1‑140 (MQ=255) cgcAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaagaa < 2:1173938/128‑1 (MQ=255) cgcAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaagaa > 1:1173938/1‑128 (MQ=255) cgcAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt > 2:1380399/1‑140 (MQ=255) cgcAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt > 2:115232/1‑140 (MQ=255) cgcAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt > 1:850648/1‑140 (MQ=255) cgcAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATG‑‑ACGCGACTGGTGCATTACGAAGAAGATcg < 1:131290/131‑1 (MQ=255) cgcAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATG‑‑ACGCGACTGGTGCATTACGAAGAAGATcg > 2:131290/1‑131 (MQ=255) aGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg < 1:956861/135‑1 (MQ=255) aGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg > 2:956861/1‑135 (MQ=255) gAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAg < 2:790235/140‑1 (MQ=255) gAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAg > 1:805711/1‑140 (MQ=255) aTCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGat < 1:1047310/140‑1 (MQ=255) aTCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGat < 1:1550843/140‑1 (MQ=255) cGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGAtatt > 2:1299956/1‑140 (MQ=255) cGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGAtatt > 1:1292312/1‑140 (MQ=255) gTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGAtatta < 2:805711/140‑1 (MQ=255) gATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTAttt < 1:237027/140‑1 (MQ=255) gATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTAttt < 2:186224/140‑1 (MQ=255) gATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTAttt < 1:1320849/140‑1 (MQ=255) gATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCAATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGATGAAGATCGCGATGCTGAAGTGGTCGATATTAttt < 2:1623214/140‑1 (MQ=255) tcatcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcc < 1:115232/140‑1 (MQ=255) catcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCTACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca < 1:144470/140‑1 (MQ=255) catcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca < 1:58433/140‑1 (MQ=255) catcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca < 1:38321/140‑1 (MQ=255) catcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca < 2:185389/140‑1 (MQ=255) catcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca < 1:1380399/140‑1 (MQ=255) catcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca < 1:842916/140‑1 (MQ=255) catcatcGGGTCGCTGGGCTATGTATCCCGCTATGTTAGTAACATCCGCGTGATGTATTTTGAGTCGAAAGCCCTGCATGGCACGCAACTGCTGCATTTCGAAGAAGATCGCGATGCTGAAGTGGTCGATATTGTTTtca < 2:330302/140‑1 (MQ=255) tcatcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTccacc < 2:992155/140‑1 (MQ=255) catcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca > 2:737947/1‑137 (MQ=255) catcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTcca < 1:737947/137‑1 (MQ=255) atcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCAt > 2:1239611/1‑140 (MQ=255) tcTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATg > 2:873189/1‑140 (MQ=255) ggTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTac > 2:764/1‑140 (MQ=255) gTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTaca > 2:1564662/1‑140 (MQ=255) gTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTaca > 2:347377/1‑140 (MQ=255) gTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTaca > 2:244238/1‑140 (MQ=255) tCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTacac > 1:158088/1‑140 (MQ=255) cTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCcg > 1:502256/1‑140 (MQ=255) cTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCCGAAGTGGTCGATATTATTTcca > 1:1041437/1‑127 (MQ=255) ggCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGt > 2:481229/1‑140 (MQ=255) gCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTg < 2:1183194/140‑1 (MQ=255) gCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTg < 1:463823/140‑1 (MQ=255) cAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGc < 1:1459880/140‑1 (MQ=255) gAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCt < 1:764/140‑1 (MQ=255) ccGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGGCGATATTATTTCCACCATGTACACCCGCGTGCCGCtgatg > 2:1609852/1‑140 (MQ=255) cGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCtgatga < 2:850648/140‑1 (MQ=255) ggCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGatgaa < 1:1119237/140‑1 (MQ=255) ggCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGatgaa > 2:1119237/1‑140 (MQ=255) gCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGatgaat < 1:882083/140‑1 (MQ=255) gCTATGGCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGatgaat < 2:908123/140‑1 (MQ=255) gCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCTCCATGTACACCCGCGTGCCGCTGATGAATGAGttt < 2:141356/140‑1 (MQ=255) gCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGttt > 2:454963/1‑140 (MQ=255) gCTGTAACATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGttt < 1:542302/140‑1 (MQ=255) aCATCCGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGaa < 1:166956/140‑1 (MQ=255) ccGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATAcc > 2:1037226/1‑140 (MQ=255) ccGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATAcc > 1:976002/1‑140 (MQ=255) ccGCGCGATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATAcc > 2:1244276/1‑140 (MQ=255) cgcgcgATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATAccc > 1:989978/1‑140 (MQ=255) gATGTACCATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATc < 1:347377/140‑1 (MQ=255) cATGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGcc < 1:466944/140‑1 (MQ=255) aTGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCcg < 1:1244276/140‑1 (MQ=255) aTGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCcg < 2:976002/140‑1 (MQ=255) tGCGTCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCcgc < 2:989978/140‑1 (MQ=255) gcgtcgAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCcgcg < 1:66045/140‑1 (MQ=255) | TCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCG > NC_000913/3223883‑3224155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |