Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,443	G→T	R297L (CGG→CTG)	modC →	molybdate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,443	0	G	T	100.0%	208.7 / NA	58	R297L (CGG→CTG)	modC	molybdate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/25);  total (33/25)

CGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTA  >  NC_000913/797308‑797578                                                                                                                                        |                                                                                                                                        cgctggGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCa                                                                                                                                     >  1:452614/1‑140 (MQ=255)    tggGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCaaaa                                                                                                                                  <  1:1004626/140‑1 (MQ=255)    ggggCGATCAGCTTTTGTGGGTCAATAGGCGGGACGACCCGCTGCAGGCTGCGCTCCGTATCCCCATCCAGGCTTCCGATTTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCaaaa                                                                                                                                  <  2:786322/139‑1 (MQ=255)    ggggCGATCAGCTTTTGTGGGTCAATAAGCGGGACGACCCGCTGCAAGCTGCGCTACGTACCCGCATTCAGGCTTCCGATTTTTTTCTGTTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCaaaa                                                                                                                                  <  2:1170142/139‑1 (MQ=255)      ggCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAgt                                                                                                                                >  1:663161/1‑140 (MQ=255)      ggCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGCTTTACAACCGCCGCAGCAAACCACCATTCGTAACGCATTGCTGGCAAAAgt                                                                                                                                >  2:790137/1‑140 (MQ=255)          aTCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAgttgtt                                                                                                                            <  1:564119/140‑1 (MQ=255)                   tgggggTAAATAAGCTGGCCGAACCGCTGCAGGTTGCGCTACGTCCCCGCATCCAGGTTTCCGATGTTTCTTTGGTTTTAAAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTAt                                                                                                                   <  2:1414165/137‑1 (MQ=255)                    gtgGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATg                                                                                                                  >  1:1194312/1‑140 (MQ=255)                    gtgGGTAAATAAGCGGAACAAACCGGCGAAAGCTGCGCTACTTTCCCGCTTTCGGGTTTCTGGTGTTTTTTTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATg                                                                                                                  <  2:164425/140‑1 (MQ=255)                     tgGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATga                                                                                                                 >  2:1500954/1‑140 (MQ=255)                         tCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATgacgac                                                                                                             >  1:914034/1‑140 (MQ=255)                           aaTAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACaa                                                                                                           >  2:1507699/1‑140 (MQ=255)                              aaGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACgg                                                                                                        <  1:1271494/140‑1 (MQ=255)                              aaGCGGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCTTTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACgg                                                                                                        <  2:110772/140‑1 (MQ=255)                                   ggccGACCCGCTGCAAGCTGCGCTACGTACCCGCATTCAGGCTTCCGATTTTTCTCTGTTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAgg                                                                                                   <  2:452614/137‑1 (MQ=255)                                    gACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgt                                                                                                  >  1:1520619/1‑140 (MQ=255)                                       gAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgtgga                                                                                               >  2:594787/1‑140 (MQ=255)                                       gAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgtgga                                                                                               >  2:1169168/1‑140 (MQ=255)                                         cccGCTGCAGGCTGCGCTACGTATCCGCATTCAGGCTTCCGTTGTTTCTTTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgtggaag                                                                                             <  2:153157/139‑1 (MQ=255)                                          ccGCTGCAAGCTGCGTTGGGTATCCGCATTCGGGCTTCCAATGTTTCTCTGTTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgtggaagt                                                                                            <  1:404504/140‑1 (MQ=255)                                              tGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGgtggaagtggaa                                                                                        >  1:84563/1‑140 (MQ=255)                                                    cTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGTTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGaa                                                                                  <  1:274571/140‑1 (MQ=255)                                                       cgcTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTc                                                                               >  2:1582992/1‑140 (MQ=255)                                                         cTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTcgg                                                                             <  2:1194312/140‑1 (MQ=255)                                                               aTCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTaa                                                                       >  2:1450444/1‑140 (MQ=255)                                                                 ccttaTTCAGGCTTCCATTTTTTCTTTGTTTTTAAACCCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTaaaa                                                                     <  2:1216457/136‑1 (MQ=255)                                                                 ccGCATTCAGGCTTCCGATGTTTCTCTGGTTTTAGAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTaaaa                                                                     >  1:1637885/1‑140 (MQ=255)                                                                      ttCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCtg                                                                <  1:1169168/140‑1 (MQ=255)                                                                      ttCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCtg                                                                >  1:859757/1‑140 (MQ=255)                                                                      ttCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCtg                                                                >  2:163446/1‑140 (MQ=255)                                                                         aGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTgg                                                             >  2:1209952/1‑140 (MQ=255)                                                                         aGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTgg                                                             >  1:404557/1‑140 (MQ=255)                                                                         aGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTgg                                                             >  1:96523/1‑140 (MQ=255)                                                                           gtttCCGATGTTTCAGTGGTTTCATAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGgc                                                           <  2:1338403/138‑1 (MQ=255)                                                                           gCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGgc                                                           >  1:1384522/1‑140 (MQ=255)                                                                            cTTCCGATGTTTCTCTGTTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGgcg                                                          <  1:936769/140‑1 (MQ=255)                                                                              tCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGcgcg                                                        >  1:413524/1‑140 (MQ=255)                                                                                 gATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTAt                                                     <  1:1507699/140‑1 (MQ=255)                                                                                       tctcTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGccc                                               >  2:1290235/1‑140 (MQ=255)                                                                                       tctcTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGccc                                               >  1:924898/1‑140 (MQ=255)                                                                                        ctctGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCg                                              <  1:688764/140‑1 (MQ=255)                                                                                             tttttACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGc                                         <  1:594787/139‑1 (MQ=255)                                                                                             gTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGc                                         <  2:413524/140‑1 (MQ=255)                                                                                                       cgccgcAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGaa                               >  1:999936/1‑140 (MQ=255)                                                                                                        gccgcAGCAAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAAc                              >  1:179612/1‑140 (MQ=255)                                                                                                               caAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGAt                       <  2:1520619/140‑1 (MQ=255)                                                                                                               caAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGAt                       <  2:38043/140‑1 (MQ=255)                                                                                                               caAACCAGCATTCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGAt                       <  2:404557/140‑1 (MQ=255)                                                                                                                         ttCGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGc             >  1:911277/1‑140 (MQ=255)                                                                                                                           cGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCt           >  2:1608281/1‑140 (MQ=255)                                                                                                                           cGTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCt           <  2:492089/140‑1 (MQ=255)                                                                                                                            gTAACGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCtg          >  2:1388486/1‑140 (MQ=255)                                                                                                                              aaCGTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCtgtg        >  2:440485/1‑140 (MQ=255)                                                                                                                                 gTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCt     >  2:1559931/1‑140 (MQ=255)                                                                                                                                 gTATTGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCt     >  1:1442213/1‑140 (MQ=255)                                                                                                                                    ttGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTa  >  1:779961/1‑140 (MQ=255)                                                                                                                                    ttGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTa  >  2:58487/1‑140 (MQ=255)                                                                                                                                    ttGCTGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTa  >  2:1292143/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CGCTGGGCGATCAGCATTTGTGGGTCAATAAGCTGGACGAACCGCTGCAAGCTGCGCTACGTATCCGCATTCAGGCTTCCGATGTTTCTCTGGTTTTACAACCGCCGCAGCAAACCAGCATTCGTAACGTATTGCGGGCAAAAGTTGTTAATAGTTATGACGACAACGGCCAGGTGGAAGTGGAACTGGAAGTCGGCGGTAAAACGCTGTGGGCGCGTATCAGCCCGTGGGCCAGGGATGAACTGGCGATCAAACCTGGCCTGTGGCTGTA  >  NC_000913/797308‑797578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑