Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,233,327	G→T	A464E (GCG→GAG)	nhaB ←	Na(+):H(+) antiporter NhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,233,327	0	G	T	100.0%	320.5 / NA	89	A464E (GCG→GAG)	nhaB	Na(+):H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/47);  total (42/47)

CCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTG  >  NC_000913/1233191‑1233462                                                                                                                                         |                                                                                                                                        ccAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGa                                                                                                                                      <  1:500/140‑1 (MQ=255) ccAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGa                                                                                                                                      <  2:302141/140‑1 (MQ=255) ccAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGa                                                                                                                                      <  2:1617121/140‑1 (MQ=255)    gCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTg                                                                                                                                   <  2:213487/140‑1 (MQ=255)    gCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTg                                                                                                                                   <  2:1684593/140‑1 (MQ=255)     cccATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGc                                                                                                                                  >  2:1452456/1‑140 (MQ=255)     cccATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGc                                                                                                                                  >  2:336115/1‑140 (MQ=255)     cccATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGc                                                                                                                                  >  1:704303/1‑140 (MQ=255)      ccATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCa                                                                                                                                 <  1:44509/140‑1 (MQ=255)          ttGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAgg                                                                                                                             >  2:1585092/1‑140 (MQ=255)              aTAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTcag                                                                                                                         <  2:176020/140‑1 (MQ=255)               tAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTcagc                                                                                                                        <  1:974672/140‑1 (MQ=255)                aaaCCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTcagca                                                                                                                       <  2:847697/140‑1 (MQ=255)                  aCCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGcagg                                                                                                                     <  1:636196/140‑1 (MQ=255)                      ttCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGcaggaaca                                                                                                                 <  1:1594605/140‑1 (MQ=255)                      ttCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGcaggaaca                                                                                                                 >  1:50749/1‑140 (MQ=255)                      ttCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGAGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGGCAGcaggaaca                                                                                                                 >  2:310914/1‑140 (MQ=255)                      ttCGGTTACAAGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGcaggaaca                                                                                                                 >  1:1491261/1‑140 (MQ=255)                       tCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGcaggaacag                                                                                                                >  1:1473985/1‑140 (MQ=255)                       tCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGcaggaacag                                                                                                                >  2:455905/1‑140 (MQ=255)                       tCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTCCACAGGCCAGcaggaacag                                                                                                                >  2:1431190/1‑140 (MQ=255)                        cGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGcaggaacagg                                                                                                               >  2:1350545/1‑140 (MQ=255)                              cAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCa                                                                                                         >  2:941081/1‑140 (MQ=255)                                   gCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACAATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTg                                                                                                    >  2:1226113/1‑140 (MQ=255)                                         gTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGtt                                                                                              >  2:586686/1‑140 (MQ=255)                                              cTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCg                                                                                         <  1:336115/140‑1 (MQ=255)                                              cTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCg                                                                                         <  2:495107/140‑1 (MQ=255)                                                   cGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCg                                                                                    >  2:213448/1‑140 (MQ=255)                                                      agagCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACg                                                                                 <  1:455905/140‑1 (MQ=255)                                                        agCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGa                                                                               >  2:829914/1‑140 (MQ=255)                                                           aaGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTTACCGTTCGGCGTAGCGACGGAggg                                                                            <  2:1491261/140‑1 (MQ=255)                                                           aaGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAggg                                                                            <  2:529050/140‑1 (MQ=255)                                                           aaGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAggg                                                                            <  1:1431190/140‑1 (MQ=255)                                                             gCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCa                                                                          >  2:330507/1‑140 (MQ=255)                                                              ccGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAg                                                                         <  1:996447/140‑1 (MQ=255)                                                                gacgaGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGAt                                                                       <  1:735850/140‑1 (MQ=255)                                                                gacgaGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGAt                                                                       <  1:941081/140‑1 (MQ=255)                                                                    aGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGt                                                                   <  1:213448/140‑1 (MQ=255)                                                                     gtgtCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTa                                                                  <  2:724927/140‑1 (MQ=255)                                                                     gtgtCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTa                                                                  <  2:1477244/140‑1 (MQ=255)                                                                     gtgtCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTa                                                                  <  1:310914/140‑1 (MQ=255)                                                                      tgtCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTAc                                                                 <  1:1659018/140‑1 (MQ=255)                                                                      tgtCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTAc                                                                 <  2:78439/140‑1 (MQ=255)                                                                       gtCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTAcc                                                                <  2:704303/140‑1 (MQ=255)                                                                           ggACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTa                                                            >  2:86597/1‑140 (MQ=255)                                                                                aGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAAt                                                       >  1:235564/1‑140 (MQ=255)                                                                                  ggTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATgg                                                     >  1:1038198/1‑140 (MQ=255)                                                                                  ggTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATgg                                                     <  2:50749/140‑1 (MQ=255)                                                                                   gtgtAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGc                                                    <  1:1350545/140‑1 (MQ=255)                                                                                    tgtAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCg                                                   >  1:1132259/1‑140 (MQ=255)                                                                                      tAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGAc                                                 >  2:94203/1‑140 (MQ=255)                                                                                             gggCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCcagc                                          >  2:1010454/1‑140 (MQ=255)                                                                                              ggCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGcc                                             <  2:1269730/136‑1 (MQ=255)                                                                                              ggCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGcc                                             >  1:1269730/1‑136 (MQ=255)                                                                                              ggCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCcagca                                         >  2:707089/1‑140 (MQ=255)                                                                                              ggCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCcagca                                         >  1:1518868/1‑140 (MQ=255)                                                                                                 cATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCt                                      <  1:1452456/140‑1 (MQ=255)                                                                                                 cATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCt                                      <  1:94203/140‑1 (MQ=255)                                                                                                 cATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCt                                      <  1:207595/140‑1 (MQ=255)                                                                                                   tCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCg                                    >  2:1431921/1‑140 (MQ=255)                                                                                                   tCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCg                                    >  1:912039/1‑140 (MQ=255)                                                                                                    ccACACCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGGAGCGACGGAGGGCAGATTGGTATCGGTATTCATGGCGACCGCCAGCAGCCCGt                                   >  2:816013/1‑140 (MQ=255)                                                                                                     cacaCCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTa                                  <  1:1359370/140‑1 (MQ=255)                                                                                                     cacaCCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTa                                  <  2:202609/140‑1 (MQ=255)                                                                                                      acacCATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTAt                                 <  1:393545/140‑1 (MQ=255)                                                                                                         caaTGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCAAGCAGCTCGTATTGc                              <  1:1226113/138‑1 (MQ=255)                                                                                                          cATGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCt                             >  1:1596311/1‑140 (MQ=255)                                                                                                            tGCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTc                           >  1:104353/1‑140 (MQ=255)                                                                                                             gCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCa                          >  1:1197102/1‑140 (MQ=255)                                                                                                             gCGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCa                          >  2:593202/1‑140 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCCGCAGGCACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCaa                         >  2:812181/1‑140 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCagcagc                                       <  1:169033/126‑1 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCagcagc                                       >  2:169033/1‑126 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGt                                   >  2:1419022/1‑130 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGt                                   <  1:1419022/130‑1 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGc                              >  2:1325789/1‑135 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGc                              <  1:1325789/135‑1 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCaa                         >  1:686045/1‑140 (MQ=255)                                                                                                              cGGCCATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCaa                         >  1:724294/1‑140 (MQ=255)                                                                                                                  cATAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTg                     >  2:1125246/1‑140 (MQ=255)                                                                                                                    tAAGAGAGGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGAt                   >  2:206577/1‑140 (MQ=255)                                                                                                                      agagagGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAg                 <  2:70037/140‑1 (MQ=255)                                                                                                                      agagagGCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAg                 <  2:1518868/140‑1 (MQ=255)                                                                                                                           ggCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGc                              >  2:543310/1‑122 (MQ=255)                                                                                                                           ggCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGc                              <  1:543310/122‑1 (MQ=255)                                                                                                                           ggCGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCa            >  2:1662350/1‑140 (MQ=255)                                                                                                                            gCGAATCAATGGCTCGAGTGCAGAGGGCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCAc           >  2:1607267/1‑140 (MQ=255)                                                                                                                             cGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACt          <  1:829914/140‑1 (MQ=255)                                                                                                                             cGAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACt          <  2:1596311/140‑1 (MQ=255)                                                                                                                              gAATCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACtt         <  1:593202/140‑1 (MQ=255)                                                                                                                               aaTCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACttt        >  1:469032/1‑140 (MQ=255)                                                                                                                               aaTCAATGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACttt        >  2:265793/1‑140 (MQ=255)                                                                                                                                     tGGCTCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTg  <  2:724294/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTG  >  NC_000913/1233191‑1233462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑