Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,390,202	C→A	G133C (GGC→TGC)	mpaA ←	murein tripeptide amidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,390,202	0	C	A	100.0%	248.4 / NA	69	G133C (GGC→TGC)	mpaA	murein tripeptide amidase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/33);  total (36/33)

GCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGT  >  NC_000913/1390076‑1390335                                                                                                                               |                                                                                                                                      gccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCt                                                                                                                          <  1:1031032/140‑1 (MQ=255)   caTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGt                                                                                                                        <  1:23841/140‑1 (MQ=255)   caTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGt                                                                                                                        <  2:1664021/140‑1 (MQ=255)    aTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTc                                                                                                                       <  2:1579867/140‑1 (MQ=255)    aTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTc                                                                                                                       <  1:99025/140‑1 (MQ=255)    aTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTc                                                                                                                       <  2:531844/140‑1 (MQ=255)      tCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAg                                                                                                                     <  2:391118/140‑1 (MQ=255)             aTTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACg                                                                                                              >  1:857000/1‑140 (MQ=255)                cGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACa                                                                                                           >  2:279971/1‑140 (MQ=255)                     tgtCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATcgcg                                                                                                      <  1:279971/140‑1 (MQ=255)                       tCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGtt                                                                                                    >  2:1122497/1‑140 (MQ=255)                          gggATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGttctt                                                                                                 <  1:1370821/140‑1 (MQ=255)                                 tCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACa                                                                                                           >  2:1113031/1‑123 (MQ=255)                                 tCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACa                                                                                                           <  1:1113031/123‑1 (MQ=255)                                 tCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcggc                                                                                          >  1:861256/1‑140 (MQ=255)                                 tCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcggc                                                                                          >  1:658387/1‑140 (MQ=255)                                  cAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcggcg                                                                                         <  2:1466249/140‑1 (MQ=255)                                     tACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTg                                                                                      >  2:19573/1‑140 (MQ=255)                                      aCAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGt                                                                                     >  2:542118/1‑140 (MQ=255)                                        aGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTc                                                                                   >  2:855793/1‑140 (MQ=255)                                          gCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 >  1:1074375/1‑140 (MQ=255)                                          gCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 >  2:612704/1‑140 (MQ=255)                                          gCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 >  1:77628/1‑140 (MQ=255)                                          gCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 >  1:749423/1‑140 (MQ=255)                                          gCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 >  1:458218/1‑140 (MQ=255)                                          gCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 >  1:377226/1‑140 (MQ=255)                                             aGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCg                                                                              <  1:1368533/140‑1 (MQ=255)                                              gTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGa                                                                             >  2:1382079/1‑140 (MQ=255)                                                    cGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACg                                                                       >  2:28205/1‑140 (MQ=255)                                                    cGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACg                                                                       <  1:1506504/140‑1 (MQ=255)                                                    cGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACg                                                                       >  1:1260799/1‑140 (MQ=255)                                                    cGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACg                                                                       >  1:1539655/1‑140 (MQ=255)                                                      tGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 >  1:1647115/1‑128 (MQ=255)                                                      tGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                 <  2:1647115/128‑1 (MQ=255)                                                      tGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCg                                                                              >  1:138200/1‑131 (MQ=255)                                                      tGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCg                                                                              <  2:138200/131‑1 (MQ=255)                                                      tGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGt                                                                     >  2:1629592/1‑140 (MQ=255)                                                         aaTGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTc                                                                  <  1:233363/140‑1 (MQ=255)                                                           tGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCAc                                                                <  2:861256/140‑1 (MQ=255)                                                            gagaCCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCAcc                                                               >  1:291849/1‑140 (MQ=255)                                                            gagaCCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCAcc                                                               >  2:1004347/1‑140 (MQ=255)                                                                   cccAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACcttccttc                                                        >  1:789025/1‑140 (MQ=255)                                                                             ttGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcgccg                                              >  1:1683002/1‑140 (MQ=255)                                                                              tGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcgccgc                                             >  2:200161/1‑140 (MQ=255)                                                                                      gtgtATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGt                                     >  1:158516/1‑140 (MQ=255)                                                                                        gtATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGttt                                   <  1:612704/140‑1 (MQ=255)                                                                                        gtATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGttt                                   <  2:1095271/140‑1 (MQ=255)                                                                                              gCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGttt                             <  2:458218/140‑1 (MQ=255)                                                                                               cTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTa                            <  1:1382079/140‑1 (MQ=255)                                                                                                   cacaGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATc                        >  2:647025/1‑140 (MQ=255)                                                                                                   cacaGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATc                        >  1:317215/1‑140 (MQ=255)                                                                                                   cacaGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATc                        >  2:478230/1‑140 (MQ=255)                                                                                                    acaGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATcc                       <  2:1074375/140‑1 (MQ=255)                                                                                                    acaGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATcc                       <  1:855793/140‑1 (MQ=255)                                                                                                     caGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCca                      <  1:379407/140‑1 (MQ=255)                                                                                                      aGCGCCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCcac                     <  2:1260799/140‑1 (MQ=255)                                                                                                        cgcCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGtt                              <  2:939755/129‑1 (MQ=255)                                                                                                        cgcCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGtt                              >  1:939755/1‑129 (MQ=255)                                                                                                        cgcCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCacac                   >  1:1478389/1‑140 (MQ=255)                                                                                                        cgcCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCacac                   <  1:1629592/140‑1 (MQ=255)                                                                                                        cgcCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCacac                   >  2:832703/1‑140 (MQ=255)                                                                                                        cgcCTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCacac                   >  1:666668/1‑140 (MQ=255)                                                                                                           cTGGGTTTCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCAt                <  1:832703/140‑1 (MQ=255)                                                                                                           cTGGGTTCCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGGTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCAt                <  2:377226/140‑1 (MQ=255)                                                                                                                tttCAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCa           <  2:666668/140‑1 (MQ=255)                                                                                                                   cAGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTg        <  2:857000/140‑1 (MQ=255)                                                                                                                    aGGTTCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTgg       >  1:270765/1‑140 (MQ=255)                                                                                                                       ttCGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGccc    <  1:478230/140‑1 (MQ=255)                                                                                                                         cGGAGCAAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGt  <  2:1539655/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                      GCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGT  >  NC_000913/1390076‑1390335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑