Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	24.2%	145.4 / 23.8	66	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (35/15);  new base C (0/16);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 3.51e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445419‑445692                                                                                                                                            |                                                                                                                                        tAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATa                                                                                                                                         >  1:1573204/1‑140 (MQ=255)  aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                        <  2:585378/140‑1 (MQ=255)     aaaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCCGCCCGCATTTCGCCCGCGAGAAAAAaccc                                                                                                                                     >  2:2630194/1‑140 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAcccccc                                                                                                                                   >  2:1161207/1‑139 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:2442284/140‑1 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:2864710/140‑1 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTCCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:1583733/140‑1 (MQ=255)         gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                 <  1:111630/140‑1 (MQ=255)         gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                 <  2:622764/140‑1 (MQ=255)           aCTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑cc                                                                                                                              <  2:504275/140‑1 (MQ=255)               ttAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCa                                                                                                                          <  1:2575468/140‑1 (MQ=255)                    gcttCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATCCGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACAt                                                                                                                     <  2:573814/137‑1 (MQ=255)                    gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACAt                                                                                                                     <  1:2725855/140‑1 (MQ=255)                       tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCGA‑CCGTCAAACATGcc                                                                                                                  >  1:1849812/1‑140 (MQ=255)                         gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGGCTGGCAGCCCCACTGCCGCCCGCATTTCGCCAGCGGGAAAAAACCCCCGA‑CCGCCAAAAATACCGt                                                                                                                >  2:710773/1‑140 (MQ=255)                         gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   >  1:2017828/1‑122 (MQ=255)                         gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:2017828/122‑1 (MQ=255)                                 cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAg                                                                                                        <  1:710773/140‑1 (MQ=255)                                 cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAg                                                                                                        <  2:2734969/140‑1 (MQ=255)                                          ccGGGTTTTAAATGCGAAAACTGTTGACATGCGGGGTTTTTTTCAGGGTACTGGCGCCTTTCAGCCACACTGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCTGC‑CCGCAAAACATGCCGTTAATCCAGCCGCCCAGc                                                                                               <  2:2992883/140‑1 (MQ=255)                                              gATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCCCACTGCGGCCAGCATTTCGCCAGCGAGAAATACCCCCCGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAAc                                                                                           >  2:1124042/1‑140 (MQ=255)                                                        cgcAAACTGCTGACATACGGCGGTTCTTTGATTGTAATGGCAGGCGACACCCCCACACCAGCCGGCATTGCGGCGGCGAGCAACACCCCCTGC‑CCGACAAACACGCCCTTAAACCAACCGCCCAGCGAACCGGCACTccc                                                                                 >  2:1822399/1‑138 (MQ=255)                                                        cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                 <  1:818629/140‑1 (MQ=255)                                                        cgcAAACTGCTGACATACGGCGGTTCTTTCATGGGACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                 >  1:536656/1‑140 (MQ=255)                                                          aaacctgctgACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                               <  2:1411716/136‑1 (MQ=255)                                                          aaaaCTGCTAACATCCGGCGGTCTTTTCATGGTACTGGCGACTGTCAGCCCCACGGCGGCAAGCATTGCGCCAGCGAGAAATCCCCCCGGA‑CCGAAAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                               <  2:894940/139‑1 (MQ=255)                                                            aaCGGCTGACATACGGGGTTTCTTTCATGGTACTGGCGACTGTCAGCCACCCGGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacg                                                                             <  2:1849812/140‑1 (MQ=255)                                                               tgctgACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacgcca                                                                          <  1:1161207/140‑1 (MQ=255)                                                                    aCATACGGCGGTTCTTTCATGGTACTGGCGACTGTCCGCCACACTGCGGCCAGCTTGGCGCCGGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                     <  2:3051836/140‑1 (MQ=255)                                                                         gggcggTTCTTTCATGGTCCTGGCGACTGTCAGCCACACTGCGGCAACAATTGCGCCAGCGAGAAATCCCCCCTGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                <  2:1258723/139‑1 (MQ=255)                                                                         cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                <  2:2102478/140‑1 (MQ=255)                                                                         cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                >  1:832598/1‑140 (MQ=255)                                                                              gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                           >  1:888823/1‑140 (MQ=255)                                                                              gTTCTTTCATGGTACTGGCGACTGTCAACCACACCGCCGCCCGCATTGCGCCCGCGAGAAATAACCCCTGA‑CCGTCAAACAAGCCCTTAAACCAGCCGCCCCACGAACCCCCAAACGCCCCGCCAAGAAActggccgggg                                                           >  2:974386/1‑138 (MQ=255)                                                                              gTTCTTCCATGGTCCTGGCGTCGCCCCCCCACTCTGCGGCAGCCATGGCGCCGGCAAAAAATCCCCCCGCA‑CCGTCAACGATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                           <  2:153964/140‑1 (MQ=255)                                                                               ttttttCATGTTATTGGCGCCTGTCAGCCACCCTGGGGCCAGCATTGCGCCAGGGAGAAATCCCCCCTGA‑CCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtgg                                                          <  2:157212/137‑1 (MQ=255)                                                                               ttCTTTAAGGGTACTGGCGCCTGTCAGCCACCCTGCGGCCAGCTTGGCGCAAGCAAGAAATCCCCCCTGA‑CCTTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtgg                                                          <  2:516885/140‑1 (MQ=255)                                                                                     cATGGTACGGGCGACTTTCAGCCACATTGCGGCCACCATTGCCCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaaa                                                    <  1:1591797/140‑1 (MQ=255)                                                                                        ggTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAacac                                                 >  1:1786997/1‑140 (MQ=255)                                                                                           aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                              >  1:852555/1‑140 (MQ=255)                                                                                            cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                             >  1:2069050/1‑140 (MQ=255)                                                                                             tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                            >  2:1388749/1‑140 (MQ=255)                                                                                             tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGGAAACACCCATc                                            >  1:2500956/1‑140 (MQ=255)                                                                                              ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                           >  2:513131/1‑140 (MQ=255)                                                                                               gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                          >  2:1374172/1‑140 (MQ=255)                                                                                                 gACTGTCAGCCCCCCTGGGGCCAGCATCGCGCCAGCGAGAAATCCCCCCTGC‑CCGTCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                        <  2:536656/140‑1 (MQ=255)                                                                                                  ccTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                       <  1:1851702/139‑1 (MQ=255)                                                                                                   cTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      >  2:253085/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  1:2829564/139‑1 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      >  2:2829564/1‑139 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  2:1224681/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  2:668180/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  2:1960853/1‑140 (MQ=255)                                                                                                      tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                   >  1:247489/1‑140 (MQ=255)                                                                                                      tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                   >  2:1876399/1‑140 (MQ=255)                                                                                                      tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                   >  1:34403/1‑140 (MQ=255)                                                                                                      tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                   >  1:625607/1‑140 (MQ=255)                                                                                                        aGCCACACTGCGGCCAGCATTGCGCCGGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGCTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCCTCGCCGTACCttt                                 >  1:2512562/1‑140 (MQ=255)                                                                                                             cacTGCGGCCACCTTTGCCCCAGCGAAAAATCCCCCCGGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAc                            <  2:1976546/140‑1 (MQ=255)                                                                                                                tGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCACCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                         <  1:2283325/140‑1 (MQ=255)                                                                                                                tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                         >  2:467592/1‑124 (MQ=255)                                                                                                                tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                         <  1:467592/124‑1 (MQ=255)                                                                                                                tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                         >  2:2673618/1‑140 (MQ=255)                                                                                                                  cGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                       <  1:1224681/140‑1 (MQ=255)                                                                                                                  cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                       >  1:1976546/1‑140 (MQ=255)                                                                                                                  cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                       >  1:673083/1‑140 (MQ=255)                                                                                                                  cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                       >  2:2726067/1‑140 (MQ=255)                                                                                                                  cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                       >  2:1809058/1‑140 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     <  1:1388749/140‑1 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     >  1:2712908/1‑140 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     <  1:1746603/140‑1 (MQ=255)                                                                                                                     ccAGCTTGGCGCCAGCGAAAAACCCCCCCTGA‑CCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                    <  2:241487/140‑1 (MQ=255)                                                                                                                       aGCTTTGCGCCAGCGAGAAAGCCCCCCGGC‑CCTTAAAACATGCCGTTAATCCAGCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  2:247489/140‑1 (MQ=255)                                                                                                                       aGCATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  1:2637823/140‑1 (MQ=255)                                                                                                                        gCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                 >  2:2990806/1‑140 (MQ=255)                                                                                                                        gCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                 <  1:513131/140‑1 (MQ=255)                                                                                                                         cATTGCGCCAGCGAGAAATCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                <  1:2525041/140‑1 (MQ=255)                                                                                                                         cATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                >  2:2202257/1‑140 (MQ=255)                                                                                                                           ttGCGCCGGCGAGAAATCCCCCTTGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:2827876/140‑1 (MQ=255)                                                                                                                           ttGCGCCAGCGAGAAATACCCCCTGA‑CCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:2630194/140‑1 (MQ=255)                                                                                                                           ttGCGCCAGCGAGAAATACCCCCTGA‑CCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:2899047/140‑1 (MQ=255)                                                                                                                            tGCGCCAGCGAAAAATCCCCCCTGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt             <  2:888823/140‑1 (MQ=255)                                                                                                                             gcggCAGCGAGAAAT‑CCCCCTGACCCATAAAACAGGCCGTTAATCCAGCCGCCCAGCGATCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  2:714631/140‑1 (MQ=255)                                                                                                                             gcgcCGGGGAGAAATCCCCCCTGC‑CCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  2:1573204/140‑1 (MQ=255)                                                                                                                             gccccAGCGAAAAATCCCCCCTGA‑CCTTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  2:2915051/137‑1 (MQ=255)                                                                                                                             ccgcCGGCGAAAAATCCCCCCTGC‑CCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  2:2069050/139‑1 (MQ=255)                                                                                                                              cgcCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           >  1:1620376/1‑140 (MQ=255)                                                                                                                              cgcCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           >  1:241487/1‑140 (MQ=255)                                                                                                                                  ggCGAGAAATCCCCCCGGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  2:832598/139‑1 (MQ=255)                                                                                                                                  aGCGAGAAATCCCCCCTGA‑CCGTCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  1:298560/140‑1 (MQ=255)                                                                                                                                  aGCGAAAAATCCCCCCGGC‑CCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  2:852555/140‑1 (MQ=255)                                                                                                                                   gCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt      >  1:1601726/1‑140 (MQ=255)                                                                                                                                   gCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt      >  1:1992985/1‑140 (MQ=255)                                                                                                                                       aaaaTCCCCCCTGC‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt  <  1:1214687/139‑1 (MQ=255)                                                                                                                                       aaaaTCCCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt  <  2:1786997/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        TAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGA‑CCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445419‑445692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑