| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,361,714 | C→A | R199S (CGC→AGC) | puuD → | gamma‑glutamyl‑gamma‑aminobutyrate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,361,714 | 0 | C | A | 98.6% | 517.2 / ‑0.5 | 145 | R199S (CGC→AGC) | puuD | gamma‑glutamyl‑gamma‑aminobutyrate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (68/75); total (69/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 154 total aligned reads displayed.
TGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTT > NC_000913/1361578‑1361849 | gCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAgctagctc < 1:1224588/140‑1 (MQ=255) aCCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCtagctcgc > 2:1888381/1‑140 (MQ=255) aCCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCtagctcgc > 1:2237485/1‑140 (MQ=255) cGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTGGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGCTGCGTGTTGAAGCTAGCTCGCCg > 2:291518/1‑140 (MQ=255) cGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCg < 1:2878615/140‑1 (MQ=255) cGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCg > 2:2353900/1‑140 (MQ=255) cGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCg > 1:1875825/1‑140 (MQ=255) gTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCgg < 2:376022/140‑1 (MQ=255) tcacATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGa < 2:2962110/137‑1 (MQ=255) tCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGa < 2:1010560/140‑1 (MQ=255) tCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGa < 2:2291439/140‑1 (MQ=255) cATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATgg > 1:859437/1‑140 (MQ=255) cATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATgg > 2:1378142/1‑140 (MQ=255) tCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTg > 2:561544/1‑140 (MQ=255) cAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGa < 1:1321630/140‑1 (MQ=255) aGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAg < 2:642098/140‑1 (MQ=255) tGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCgg < 1:2853506/140‑1 (MQ=255) aaGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGtt > 1:1231675/1‑140 (MQ=255) agagGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGttt > 2:2989230/1‑139 (MQ=255) agaaGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTa > 2:1674514/1‑140 (MQ=255) gagGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAg < 1:423516/140‑1 (MQ=255) ggggggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAg < 2:1388705/125‑1 (MQ=255) ggggggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAg > 1:1388705/1‑125 (MQ=255) gggggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCgg > 2:3060558/1‑139 (MQ=255) gtggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtc > 1:1352317/3‑140 (MQ=255) ggggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtc < 2:938682/140‑1 (MQ=255) ggggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtc > 1:1194169/1‑140 (MQ=255) ggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtcat < 1:1888381/140‑1 (MQ=255) aTTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtcatca > 1:2605083/1‑140 (MQ=255) aTTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGtcatc < 1:1670375/139‑1 (MQ=255) tGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTTGCGTCCTCAaacat > 2:766629/1‑140 (MQ=255) tCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATcc > 2:153741/1‑140 (MQ=255) tCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATcc > 2:1591117/1‑140 (MQ=255) tGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCtt > 2:1758201/1‑140 (MQ=255) tGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCtt > 2:57398/1‑140 (MQ=255) tGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCtt > 2:1954595/1‑140 (MQ=255) tGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCtt > 2:2819342/1‑140 (MQ=255) gCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCttt > 2:2860931/1‑140 (MQ=255) gttgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTg > 2:2081769/1‑140 (MQ=255) ttgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgc < 2:2605083/140‑1 (MQ=255) tgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTTGCGGCCTCAATCATCCTTTTtcg > 2:747409/1‑140 (MQ=255) tgttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgcg > 1:261343/1‑140 (MQ=255) gttACCTGAGTGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgcgc < 2:2116655/140‑1 (MQ=255) gttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgcgc < 2:1854984/140‑1 (MQ=255) gttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgcgc < 2:261343/140‑1 (MQ=255) gttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgcgc < 2:1181208/140‑1 (MQ=255) gttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgcgc < 2:547593/140‑1 (MQ=255) tACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTg > 2:343174/1‑140 (MQ=255) aaTGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTa > 1:2556771/1‑140 (MQ=255) aaTGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTa > 2:1933393/1‑140 (MQ=255) aTGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTAc > 1:573645/1‑140 (MQ=255) tGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTgcgc > 1:2932710/1‑130 (MQ=255) tGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACa < 2:159511/140‑1 (MQ=255) tAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGt < 1:1758201/140‑1 (MQ=255) gCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTgg > 2:3049751/1‑140 (MQ=255) cAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGc > 1:972720/1‑140 (MQ=255) cAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGc > 1:1628514/1‑140 (MQ=255) cTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCAcc < 1:2819342/140‑1 (MQ=255) cTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCAcc > 1:1118244/1‑140 (MQ=255) cTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCAcc < 1:1502293/140‑1 (MQ=255) cTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCAcc < 1:29358/140‑1 (MQ=255) cTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCAcc < 2:1314931/140‑1 (MQ=255) gggTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGaa < 1:561544/140‑1 (MQ=255) gggTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGaa < 1:739250/140‑1 (MQ=255) gggTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGaa < 1:679281/140‑1 (MQ=255) aaaCTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGa < 1:2563119/140‑1 (MQ=255) aaCTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGaa < 1:1151196/140‑1 (MQ=255) aaCTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGaa > 1:1965654/1‑140 (MQ=255) ctACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGaa < 2:1931258/134‑1 (MQ=255) ctACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGaa > 1:1931258/1‑134 (MQ=255) ctACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAg > 2:2170767/1‑140 (MQ=255) tACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGc > 2:746072/1‑140 (MQ=255) ggACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTa > 2:2375677/1‑140 (MQ=255) gACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGGCATCAATAACCCTTTTTTGCTAGGCGCACAGTGGGGCCCCGGAAGGGACAGTAACgcggac > 2:1545309/1‑135 (MQ=255) cAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTAcgc > 2:1887127/1‑140 (MQ=255) cAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTAcgc > 1:1067446/1‑140 (MQ=255) aGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACgcgc > 1:1831623/1‑140 (MQ=255) aGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGGACgcgc > 1:2081382/1‑140 (MQ=255) ggggCGAAGGTCGTTATTCCACGGTTGCGTGTTGAAGCTAGCTCGACGGATGGCTTAGGTGAGCCGGTTAGTGTCATTAACCACCGTTTTGCTCTGGGCGTACAGTTCCGCCCGGAATGCAACAGTAGCGGGTACGCGCt < 2:2911305/140‑1 (MQ=255) ggggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCTTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCt < 1:2218798/140‑1 (MQ=255) ggggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCt < 1:762915/140‑1 (MQ=255) ggggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCt < 1:353954/140‑1 (MQ=255) ggggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCt < 1:397360/140‑1 (MQ=255) ggggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCt < 1:1010267/140‑1 (MQ=255) ggggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCt < 2:1067446/140‑1 (MQ=255) ggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCttt < 1:2866643/140‑1 (MQ=255) cGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTcg < 2:1965654/140‑1 (MQ=255) gAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTcgc < 2:1252689/140‑1 (MQ=255) caGGTCGTTAGCCCACCGTTGCGTGTTGAAGCTAGCCCGCCGGATGGCTTAGTTGAGGCGGTTTATCTGATCAATCATTCTTTTTTGCTCGGAGTACACCGGCACCCGGGCGGGGACCGAATCGTGTAAGCACTTTcgcc > 2:218604/2‑139 (MQ=255) aaGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTcgcg > 1:308519/1‑140 (MQ=255) aaGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGGACAGGGGGACCCGGGATGGAAAAGTAGCGAGGACGCGCTTTcgcg > 2:1174227/1‑140 (MQ=255) aaGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCCTCATCAATCATCCTTTTTCGCCGGGCGTACAGGGGGACCCCGAATGGAACAGTAGCGAGGAAGCGCTTTcccg > 2:386553/1‑140 (MQ=255) gTTAGCCCACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTGATCCATCATCCTTTTTCGCCTGGCGTGAAGGGGGACCCGGAATAGAACAGAAGAGAGAACGCGCTTTCGCGGATAtt > 2:1977241/1‑140 (MQ=255) cccACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCg < 2:8087/140‑1 (MQ=255) cACGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAg < 1:1028823/140‑1 (MQ=255) cGGTTGCGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGGATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAggg > 1:2524561/1‑140 (MQ=255) cGTGTTGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTAt > 2:2041985/1‑140 (MQ=255) tGAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCg < 1:3049751/140‑1 (MQ=255) gAAGCTAGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGc < 1:2041985/140‑1 (MQ=255) agctagctCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCtt < 1:1545309/140‑1 (MQ=255) | TGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCGTCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTT > NC_000913/1361578‑1361849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |