Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,116,917 T→C D37D (GAT→GAC)  yceA → UPF0176 protein YceA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,116,9170TC99.0% 724.9 / ‑3.1 192D37D (GAT→GAC) yceAUPF0176 protein YceA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (83/107);  total (84/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

Only 100 of 193 total aligned reads displayed.

TCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCGCGCGCAG  >  NC_000913/1116782‑1117052
                                                                                                                                       |                                                                                                                                       
tCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTa                                                                                                                                     >  1:22268/1‑140 (MQ=255)
  ccATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCGTTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAg                                                                                                                                   <  1:1818423/140‑1 (MQ=255)
     tctcGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCg                                                                                                                                <  2:589469/140‑1 (MQ=255)
     tctcGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCg                                                                                                                                >  1:225720/1‑140 (MQ=255)
          cAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCg                                                                                                                           >  1:1266542/1‑140 (MQ=255)
          cAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCg                                                                                                                           >  1:1868647/1‑140 (MQ=255)
           aGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGt                                                                                                                          <  1:3030920/140‑1 (MQ=255)
            ggAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTg                                                                                                                         <  1:1346508/140‑1 (MQ=255)
            ggAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTg                                                                                                                         <  2:22268/140‑1 (MQ=255)
            ggAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTg                                                                                                                         <  2:2808364/140‑1 (MQ=255)
            ggAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTg                                                                                                                         <  1:2862360/140‑1 (MQ=255)
             gAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGa                                                                                                                        <  1:40712/140‑1 (MQ=255)
             gAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGa                                                                                                                        <  2:2940238/140‑1 (MQ=255)
             gAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGa                                                                                                                        <  1:1990997/140‑1 (MQ=255)
                ccGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGAGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGc                                                                                                                     <  2:1073453/140‑1 (MQ=255)
                   tACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAgg                                                                                                                                  <  2:1477290/124‑1 (MQ=255)
                   tACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCttt                                                                                                                  >  2:205813/1‑140 (MQ=255)
                   tACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCttt                                                                                                                  <  2:2306154/140‑1 (MQ=255)
                   tACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCttt                                                                                                                  >  1:2306154/1‑140 (MQ=255)
                   tACACCACGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCttt                                                                                                                  >  1:155676/1‑140 (MQ=255)
                       ccATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              <  2:1955195/140‑1 (MQ=255)
                       ccATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              <  2:1502662/140‑1 (MQ=255)
                       ccATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              <  1:997451/140‑1 (MQ=255)
                       ccACGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              <  2:155676/140‑1 (MQ=255)
                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTg                                                                                                                         <  1:2056103/126‑1 (MQ=255)
                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGc                                                                                                                     <  1:1440723/130‑1 (MQ=255)
                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAg                                                                                                           >  1:1912125/1‑140 (MQ=255)
                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAg                                                                                                           >  2:1204840/1‑140 (MQ=255)
                          tGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAg                                                                                                           >  1:2815328/1‑140 (MQ=255)
                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGc                                                                                                          >  1:2669331/1‑140 (MQ=255)
                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              >  1:1169684/1‑136 (MQ=255)
                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              <  2:1169684/136‑1 (MQ=255)
                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGc                                                                                                          >  1:2104813/1‑140 (MQ=255)
                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGc                                                                                                          >  2:13743/1‑140 (MQ=255)
                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGc                                                                                                          >  1:1810587/1‑140 (MQ=255)
                           gCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGc                                                                                                          >  1:1879191/1‑140 (MQ=255)
                                tgtTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGttt                                                                                                     >  2:1765829/1‑140 (MQ=255)
                                  ttACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTAc                                                                                                   <  2:1439728/140‑1 (MQ=255)
                                    acacAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              <  1:2675963/127‑1 (MQ=255)
                                    acacAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTatat                                                                                                              >  2:2675963/1‑127 (MQ=255)
                                          ccGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGATCCACCATTTCGTTTTACAAGTATTTCCACATCGCCGCCCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGa                                                                                           <  2:666427/140‑1 (MQ=255)
                                          ccGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGa                                                                                           >  1:2533977/1‑140 (MQ=255)
                                          ccGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGa                                                                                           >  1:2174461/1‑140 (MQ=255)
                                             cATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATg                                                                                        >  1:1583802/1‑140 (MQ=255)
                                                  ccAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGttttt                                                                                   >  2:988437/1‑140 (MQ=255)
                                                  ccAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGttttt                                                                                   >  1:291309/1‑140 (MQ=255)
                                                    aaCGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTgg                                                                                 <  2:2174461/140‑1 (MQ=255)
                                                    aaCGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTgg                                                                                 <  2:2332575/140‑1 (MQ=255)
                                                    aaCGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTgg                                                                                 <  2:1282696/140‑1 (MQ=255)
                                                     acgacgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTggg                                                                                <  2:727221/140‑1 (MQ=255)
                                                     acgacgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTggg                                                                                <  2:2363558/140‑1 (MQ=255)
                                                     acgacgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTggg                                                                                <  2:291309/140‑1 (MQ=255)
                                                     acgacgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTggg                                                                                <  1:48443/140‑1 (MQ=255)
                                                       gacgCGCTAAAAGTCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCg                                                                              <  2:2597839/140‑1 (MQ=255)
                                                       gacgCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCg                                                                              <  2:633811/140‑1 (MQ=255)
                                                         cgcgCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTgg                                                                                 >  2:2984833/1‑135 (MQ=255)
                                                         cgcgCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTgg                                                                                 <  1:2984833/135‑1 (MQ=255)
                                                          gcgcTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAgt                                                                           >  2:3076933/1‑140 (MQ=255)
                                                          gcgcTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAgt                                                                           >  2:537340/1‑140 (MQ=255)
                                                                 aaGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTg                                                                    <  2:2481758/140‑1 (MQ=255)
                                                                       aaaTGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCAt                                                              <  1:2345830/140‑1 (MQ=255)
                                                                             tGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAATTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATc                                                                      <  1:2499714/126‑1 (MQ=255)
                                                                                    gagCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAAcg                                                 >  2:2114317/1‑140 (MQ=255)
                                                                                     agCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAAcgc                                                >  1:1956313/1‑140 (MQ=255)
                                                                                        gaaccgcgaaccACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCa                                             <  1:1037768/140‑1 (MQ=255)
                                                                                           ccgcgaaccACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAAt                                          <  1:3076933/140‑1 (MQ=255)
                                                                                                aaccACCATTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCg                                     <  2:379290/140‑1 (MQ=255)
                                                                                                  ccaccaTTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTa                                   <  2:1912125/140‑1 (MQ=255)
                                                                                                     ccaTTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCt                                <  1:188738/140‑1 (MQ=255)
                                                                                                      caTTTCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTg                               >  2:500831/1‑140 (MQ=255)
                                                                                                          tCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAg                           <  2:156704/140‑1 (MQ=255)
                                                                                                          tCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAg                           <  2:1879191/140‑1 (MQ=255)
                                                                                                          tCGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAg                           <  2:2049657/140‑1 (MQ=255)
                                                                                                           cGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGc                          <  2:2815328/140‑1 (MQ=255)
                                                                                                           cGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGc                          <  1:101620/140‑1 (MQ=255)
                                                                                                           cGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGc                          <  2:1810587/140‑1 (MQ=255)
                                                                                                           cGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGc                          <  2:1350523/140‑1 (MQ=255)
                                                                                                           cGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGc                          <  1:417112/140‑1 (MQ=255)
                                                                                                           cGTTTTACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGc                          <  1:335441/140‑1 (MQ=255)
                                                                                                               ttACAAGTATTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATg                      >  1:772739/1‑140 (MQ=255)
                                                                                                                       aTTTCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACa              >  2:901687/1‑140 (MQ=255)
                                                                                                                        tttCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAg                           >  1:2526008/1‑126 (MQ=255)
                                                                                                                        tttCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAg                           <  2:2526008/126‑1 (MQ=255)
                                                                                                                          tCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACAttt           >  1:667093/1‑140 (MQ=255)
                                                                                                                          tCCACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACAttt           >  2:2758522/1‑140 (MQ=255)
                                                                                                                           ccACATCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTc          <  2:2695760/140‑1 (MQ=255)
                                                                                                                              caTCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcg       <  1:1765829/140‑1 (MQ=255)
                                                                                                                              caTCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcg       <  1:2951764/140‑1 (MQ=255)
                                                                                                                               aTCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcgc      >  1:1295998/1‑140 (MQ=255)
                                                                                                                                tCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcgcg     >  2:1843273/1‑140 (MQ=255)
                                                                                                                                tCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcgcg     <  1:901687/140‑1 (MQ=255)
                                                                                                                                tCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcgcg     <  1:318254/140‑1 (MQ=255)
                                                                                                                                tCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcgcg     <  1:2716425/140‑1 (MQ=255)
                                                                                                                                tCGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTcgcgcg     <  1:2439010/140‑1 (MQ=255)
                                                                                                                                 cGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCgcgcgc    <  1:1403682/140‑1 (MQ=255)
                                                                                                                                 cGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCgcgcgc    >  2:2635581/1‑140 (MQ=255)
                                                                                                                                 cGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCgcgcgc    <  1:577906/140‑1 (MQ=255)
                                                                                                                                 cGCCGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCgcgcgc    >  1:559045/1‑140 (MQ=255)
                                                                                                                                   ccGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCGCGCGCAg  >  2:1019740/1‑140 (MQ=255)
                                                                                                                                   ccGACCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCGCGCGCAg  >  2:1628878/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGCTTTATATCAGCTGTTTACCGCGCTGAATGTTTTTGGGCGAGTGTATCTGGCGCATGAGGGCATTAACGCGCAAATCAGCGTACCTGCGAGCAATGTTGAAACATTTCGCGCGCAG  >  NC_000913/1116782‑1117052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑