Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,918	0	A	C	28.1%	184.4 / 30.3	82	intergenic (‑129/+192)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (56/3);  new base C (0/23);  total (56/26)
Fisher's exact test for biased strand distribution p-value = 1.96e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAG  >  NC_000913/2001779‑2002051                                                                                                                                              |                                                                                                                                      agTGAATTCACGATAAACAACCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGaaaaaa‑a                                                                                                                                       >  2:1348146/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑Acccc                                                                                                                                   >  1:1896611/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑Acccc                                                                                                                                   >  1:457364/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑Acccc                                                                                                                                   >  1:844361/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCccg                                                                                                                                  >  2:1100563/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCccg                                                                                                                                  >  1:1922061/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCccg                                                                                                                                  >  1:487837/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCccg                                                                                                                                  >  1:535987/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCccg                                                                                                                                  >  2:1717139/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGGTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCccg                                                                                                                                  >  2:1599636/1‑140 (MQ=255)       ttCACGAAAAACAGCCCTGCTTTATATGAGTTATGGGCATGATTATCCTTTTCGGCGGGTTTTTAAATCGGACGTTTGGGGGGTAAAATGAGGGGTTATTTGGGGG‑TTACAGGAAAATCCCAGGCAAAAAAAA‑CCCccgc                                                                                                                                 <  2:1355745/140‑1 (MQ=255)        tCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCccgcc                                                                                                                                >  2:603837/1‑140 (MQ=255)               aaaCAGCCCTGTTTTATATGAGTTATCGGCATGATTATCCGTTTCTGCGGGGTTTTTAATCGGACGATTGGTGGGTGAAATGAGGGTTTTTTGGGGGT‑TTAAAGGTAAATCCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCg                                                                                                                         <  2:535987/140‑1 (MQ=255)                        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCAc                                                                                                                >  1:279509/1‑140 (MQ=255)                        tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCAc                                                                                                                >  1:1056388/1‑140 (MQ=255)                         gctttATATGATTTATCGGCATGATTATCCTTTTCTGCGGGTTTTTTAATCGGACGATTAGTGGGTAAAATGGGGGGTTTTTGGGGGG‑TTACAGGTAAATTCCAGGCAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACg                                                                                                               <  2:1547780/137‑1 (MQ=255)                          cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGt                                                                                                              >  1:553220/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                    >  2:113345/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                    >  1:904045/1‑140 (MQ=255)                                     ttATCGGCATGTTTATCCTTTTCTGCAGGTTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGGTTATTGGGGGG‑TTACAGTTAAATTCCAGGCAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                   <  2:92516/140‑1 (MQ=255)                                     ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGGCGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                   >  2:1520509/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCGGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                 >  1:92516/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                 >  1:1610181/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                 >  1:1520924/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                 >  1:927847/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                 >  2:1686508/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                 >  2:708751/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                 >  2:891911/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGGTTCTGACTAATTg                                                                                                 >  2:1820845/1‑140 (MQ=255)                                        tCGGCATGATTATCCGTTTCGGCGGGTTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgc                                                                                                <  2:904045/140‑1 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGGACGTTGCTGACAAATTgcg                                                                                               >  2:850277/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                               >  2:1504493/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                               >  2:1433446/1‑140 (MQ=255)                                           gCATGATTATCCTTTTCTGCGGGTTTTTTAATGGGATGATTAGTGGGTGAAATGGGGGGTTATTTGGGGG‑TTACAGGAAAATCCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCt                                                                                             <  2:1896611/140‑1 (MQ=255)                                                      cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg                                                                                  >  1:11612/1‑140 (MQ=255)                                                      cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg                                                                                  >  1:1026332/1‑140 (MQ=255)                                                      cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg                                                                                  >  2:95022/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                               >  1:1832024/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                               >  1:1109588/1‑140 (MQ=255)                                                           cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                             >  2:1091054/1‑140 (MQ=255)                                                              cAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                          >  1:644993/1‑140 (MQ=255)                                                                   tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa                                                                     >  2:1958842/1‑140 (MQ=255)                                                                           gggagaaTTAGGGGGTGAAATG‑GGGGTTTTTTGGGGGATTACGGGAAAATTCCGGGCAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                             <  1:1820845/134‑1 (MQ=255)                                                                           gggACGATTAGTGGGTGAAATGGGGGTTTTTTGGGGGG‑TTACAGTAAAATTCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                             <  2:279509/139‑1 (MQ=255)                                                                           cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                             >  1:1708955/1‑140 (MQ=255)                                                                            ggagaaTTAGGGGGTAAAATGGGGGTTTATTTGGGGG‑TTACAGGAAAATTCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                            <  2:487837/135‑1 (MQ=38)                                                                            ggACGATTAGGGGGTGAAAGGGGGGGTTATTGGGGGG‑TTACAGGTAAATCCCAGGAAGAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCTGCGTAAACGCCTTATc                                                            <  2:1832024/140‑1 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                           >  1:2071701/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                           >  1:783550/1‑140 (MQ=255)                                                                              aggATTAGTGGGTGAAATGGGGGTTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAAAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                          <  1:1348146/138‑1 (MQ=255)                                                                              aCGATTGGTGGGGAAAAGGAGGGGTTTTTGGGGGG‑TTACAGGAAATTTCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                          <  2:1520924/140‑1 (MQ=37)                                                                                aaTTAGTGGGTAAAAGGGGGGGTTATTTGGGGG‑TTACAGGAAAATTCCAGGCAGAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                        <  2:370355/139‑1 (MQ=39)                                                                                        ggTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                >  2:1710386/1‑140 (MQ=255)                                                                                        ggTAAAAGGAGGGTTTTTTGGGGGT‑TTACAGATAAATTCC‑GGCAAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                <  2:1109588/140‑1 (MQ=25)                                                                                         gtaaaaTGGGGGGTTTTTGGGGGT‑TTACAGGTAAATTCCAGGCAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                               <  1:704188/137‑1 (MQ=37)                                                                                         aagAAAGGGGGGGTTTTTTGGGGG‑TTACAGGAAAATCCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                               <  2:973339/138‑1 (MQ=37)                                                                                          tGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                              >  1:148619/1‑140 (MQ=255)                                                                                            aaaTGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                            >  2:1463849/1‑140 (MQ=255)                                                                                            aaaTGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgc                                            >  1:370355/1‑139 (MQ=255)                                                                                            aaaTGAGGGGTTATTTGGGGG‑TTACAGGCAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                            >  2:1227041/1‑140 (MQ=255)                                                                                            aaaTGAGGGGTTATTTGGGGA‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                            >  1:214550/1‑140 (MQ=255)                                                                                             aaTGGGGGTTTTTTTGGGGG‑TTAAAGGTAAATTCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                           <  2:927847/140‑1 (MQ=37)                                                                                                  ggggTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt                                      >  1:851964/1‑140 (MQ=255)                                                                                                   gggTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                     >  1:1242906/1‑140 (MQ=255)                                                                                                     tttATTGGGGGG‑TTACAGGTAAATCCCAGGCAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                   <  2:644993/139‑1 (MQ=255)                                                                                                       tATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTcctg                                 >  1:973339/1‑137 (MQ=255)                                                                                                              ggg‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                          >  1:840151/1‑140 (MQ=255)                                                                                                               gg‑TTAAAGGTAAATCCCAGGAAAAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                         <  2:11612/140‑1 (MQ=37)                                                                                                                g‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                        >  2:1889262/1‑140 (MQ=255)                                                                                                                 tttACAGGTAAATTCCAGGCAGAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                        <  1:1669693/139‑1 (MQ=255)                                                                                                                   tACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                      <  1:113345/140‑1 (MQ=255)                                                                                                                    aCAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                     >  2:1080983/1‑140 (MQ=255)                                                                                                                    aCAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                     >  2:705122/1‑140 (MQ=255)                                                                                                                     cAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                    <  1:891911/140‑1 (MQ=255)                                                                                                                     cAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                    >  2:2003968/1‑140 (MQ=255)                                                                                                                          aaaTTCCAGGCAGAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg               <  1:1686508/140‑1 (MQ=255)                                                                                                                          aaaTTCCAGCAAGAAAAAC‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg               <  2:148619/140‑1 (MQ=255)                                                                                                                           aaTTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt              >  2:357093/1‑140 (MQ=255)                                                                                                                            aTTCCAGGCAGAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTa             <  1:1091054/140‑1 (MQ=255)                                                                                                                             tcccAGGAAGAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg            <  2:553220/138‑1 (MQ=255)                                                                                                                                cAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc         >  1:1546544/1‑140 (MQ=255)                                                                                                                                cAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc         >  1:439840/1‑140 (MQ=255)                                                                                                                                aaGGAAGAAAAAA‑CCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGca         <  2:1984593/139‑2 (MQ=255)                                                                                                                                  ggCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg       >  2:5860/1‑140 (MQ=255)                                                                                                                                  ggCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg       >  1:364648/1‑140 (MQ=255)                                                                                                                                       aaaaaa‑cCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag  <  2:840151/140‑1 (MQ=255)                                                                                                                                       aaaaaa‑cCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag  <  2:1242906/140‑1 (MQ=255)                                                                                                                                       aaaaaa‑cCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag  <  2:1708955/140‑1 (MQ=255)                                                                                                                                              |                                                                                                                                      AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAA‑ACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAG  >  NC_000913/2001779‑2002051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑