BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,061,836	T→C	D329D (GAT→GAC)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,061,836	0	T	C	100.0%	431.5 / NA	113	D329D (GAT→GAC)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/60); total (53/60)

Only 100 of 114 total aligned reads displayed.

TTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACA  >  NC_000913/3061699‑3061972

tttATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGAccc                                                                                                                                        >  1:1067300/1‑140 (MQ=255)

 ttATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCg                                                                                                                                       <  1:240358/140‑1 (MQ=255)

 ttATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCg                                                                                                                                       <  2:1618381/140‑1 (MQ=255)

   aTGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGta                                                                                                                                     >  2:1461626/1‑140 (MQ=255)

   aTGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGta                                                                                                                                     <  1:618128/140‑1 (MQ=255)

    tGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGtat                                                                                                                                    >  2:379263/1‑140 (MQ=255)

        cGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaaca                                                                                                                                >  1:2065704/1‑140 (MQ=255)

        cGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaaca                                                                                                                                <  2:560608/140‑1 (MQ=255)

          tCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaacaac                                                                                                                              >  2:1772836/1‑140 (MQ=255)

          tCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaacaac                                                                                                                              >  2:1466091/1‑140 (MQ=255)

          tCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaacaac                                                                                                                              >  2:2056595/1‑140 (MQ=255)

          tCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaacaac                                                                                                                              >  1:1261248/1‑140 (MQ=255)

          tCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaacaac                                                                                                                              >  1:72809/1‑140 (MQ=255)

          tCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaa                                                                                                                                  >  1:90581/1‑136 (MQ=255)

          tCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATaa                                                                                                                                  <  2:90581/136‑1 (MQ=255)

             ccATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGtt                                                                                                                           <  2:1325336/140‑1 (MQ=255)

                tGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATc                                                                                                                        <  2:1153785/140‑1 (MQ=255)

                  ttGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCg                                                                                                                      <  1:1876498/140‑1 (MQ=255)

                   tgcgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGc                                                                                                                     >  1:232665/1‑140 (MQ=255)

                     cgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGcac                                                                                                                   <  1:1772836/140‑1 (MQ=255)

                     cgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGcac                                                                                                                   <  2:226057/140‑1 (MQ=255)

                     cgtgcgGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGcac                                                                                                                   >  2:2632/1‑140 (MQ=255)

                            cACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCAtt                                                                                                            >  2:700700/1‑140 (MQ=255)

                                    ttatGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCgct                                                                                                    <  2:1083665/140‑1 (MQ=255)

                                        ggAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCgctggct                                                                                                >  2:1505578/1‑140 (MQ=255)

                                         gAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACCGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCgctggctg                                                                                               <  1:836493/140‑1 (MQ=255)

                                          aGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGc                                                                                              >  1:1827815/1‑140 (MQ=255)

                                           gCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCg                                                                                             >  2:1669896/1‑140 (MQ=255)

                                           gCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCg                                                                                             >  1:1544386/1‑140 (MQ=255)

                                           gCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCg                                                                                             >  1:770741/1‑140 (MQ=255)

                                            cGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGa                                                                                            >  2:1880510/1‑140 (MQ=255)

                                            cGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGa                                                                                            >  1:695457/1‑140 (MQ=255)

                                            cGAAGCGGTCAGTGGATCTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGa                                                                                            >  2:771798/1‑140 (MQ=255)

                                             gAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGAc                                                                                           >  2:152722/1‑140 (MQ=255)

                                              aaGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACg                                                                                          <  1:43400/140‑1 (MQ=255)

                                               aGCGGTCAGTGGATTTCGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACGCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGc                                                                                         >  2:944378/1‑140 (MQ=255)

                                                gCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCt                                                                                        <  1:940690/140‑1 (MQ=255)

                                                   gTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTggg                                                                                     <  2:1692174/140‑1 (MQ=255)

                                                   gTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTggg                                                                                     <  2:44957/140‑1 (MQ=255)

                                                   gTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGATGCGTACCCACTGCCAGGCCTCAGGCTGGAGCCTGCCTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTggg                                                                                     <  2:236489/140‑1 (MQ=255)

                                                    tCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGc                                                                                    <  2:1514453/140‑1 (MQ=255)

                                                    tCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGc                                                                                    >  1:1826183/1‑140 (MQ=255)

                                                     cAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCg                                                                                   <  1:217835/140‑1 (MQ=255)

                                                      aGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCgg                                                                                  >  2:1788630/1‑140 (MQ=255)

                                                      aGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCgg                                                                                  <  2:1146496/140‑1 (MQ=255)

                                                         ggATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTAc                                                                               <  2:610803/140‑1 (MQ=255)

                                                           aTTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTc                                                                             <  1:1572311/140‑1 (MQ=255)

                                                              tGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                          <  2:209061/140‑1 (MQ=255)

                                                              tGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                          <  2:1871109/140‑1 (MQ=255)

                                                              tGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                          <  1:1660176/140‑1 (MQ=255)

                                                              tGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                          <  2:1236962/140‑1 (MQ=255)

                                                              tGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                          <  1:122784/140‑1 (MQ=255)

                                                              tGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGt                                                                          <  2:691351/140‑1 (MQ=255)

                                                                gcgcACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCa                                                                        <  2:1827815/140‑1 (MQ=255)

                                                                gcgcACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCa                                                                        >  2:911334/1‑140 (MQ=255)

                                                                 cgcACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCAc                                                                       <  2:939470/140‑1 (MQ=255)

                                                                  gcACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTggg                                                                                     <  2:622886/125‑1 (MQ=255)

                                                                  gcACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTggg                                                                                     >  1:622886/1‑125 (MQ=255)

                                                                   cacaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTg                                                                     <  1:1131157/140‑1 (MQ=255)

                                                                   cacaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTg                                                                     <  1:379263/140‑1 (MQ=255)

                                                                    acaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTg                                                                     <  2:342681/139‑1 (MQ=255)

                                                                    acaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTg                                                                     >  1:342681/1‑139 (MQ=255)

                                                                    acaGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGc                                                                    <  1:1669896/140‑1 (MQ=255)

                                                                     caGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCa                                                                   >  2:506828/1‑140 (MQ=255)

                                                                      aGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCAt                                                                  >  1:643766/1‑140 (MQ=255)

                                                                          cccGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCCACGCTGGGCGGTACTCAGTCACTGCATACCa                                                              >  1:1668804/1‑140 (MQ=255)

                                                                           ccGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCaa                                                             <  1:1788630/140‑1 (MQ=255)

                                                                                  cACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCttt                                                      >  2:899804/1‑140 (MQ=255)

                                                                                      ggCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACg                                                  <  1:1505578/140‑1 (MQ=255)

                                                                                             cGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCt                                           >  1:1961616/1‑140 (MQ=255)

                                                                                              gTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCtt                                          <  2:1784967/140‑1 (MQ=255)

                                                                                                     cTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGc                                   <  2:32411/140‑1 (MQ=255)

                                                                                                       gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGAc                                                   <  1:855323/122‑1 (MQ=255)

                                                                                                       gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGAc                                                   >  2:855323/1‑122 (MQ=255)

                                                                                                       gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCt                                 >  1:1452861/1‑140 (MQ=255)

                                                                                                       gCCAGACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCt                                 >  1:2049834/1‑140 (MQ=255)

                                                                                                           gACCTCAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCg                             >  2:927564/1‑140 (MQ=255)

                                                                                                                cAGGCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtc                        >  2:1135504/1‑140 (MQ=255)

                                                                                                                  ggCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTtctc                      >  1:1354081/1‑140 (MQ=255)

                                                                                                                   gCTGGAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCa                     >  1:722743/1‑140 (MQ=255)

                                                                                                                       gAGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCAc                 >  2:931244/1‑140 (MQ=255)

                                                                                                                        aGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACg                >  1:1426382/1‑140 (MQ=255)

                                                                                                                        aGCCTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACg                >  1:1224427/1‑140 (MQ=255)

                                                                                                                          ccTGACTGAACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCa              >  2:1679746/1‑140 (MQ=255)

                                                                                                                             gactgaACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTg           >  1:859113/1‑140 (MQ=255)

                                                                                                                              actgaACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGc          <  2:5893/140‑1 (MQ=255)

                                                                                                                                 gaACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCg       <  2:1961616/140‑1 (MQ=255)

                                                                                                                                 gaACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCg       <  2:722743/140‑1 (MQ=255)

                                                                                                                                 gaACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCg       <  2:1544386/140‑1 (MQ=255)

                                                                                                                                 gaACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCg       <  1:931244/140‑1 (MQ=255)

                                                                                                                                 gaACAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCg       >  2:2045266/1‑140 (MQ=255)

                                                                                                                                   aCAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCa     <  2:1452861/140‑1 (MQ=255)

                                                                                                                                   aCAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCa     <  2:188430/140‑1 (MQ=255)

                                                                                                                                   aCAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCa     <  2:729912/140‑1 (MQ=255)

                                                                                                                                   aCAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCa     <  2:1059267/140‑1 (MQ=255)

                                                                                                                                    cAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCaa    <  2:1067300/140‑1 (MQ=255)

                                                                                                                                    cAGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCaa    <  2:828954/140‑1 (MQ=255)

                                                                                                                                     aGGACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAac   <  1:876615/140‑1 (MQ=255)

                                                                                                                                      ggACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAaca  >  2:619860/1‑140 (MQ=255)

                                                                                                                                      ggACCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAaca  <  1:152722/140‑1 (MQ=255)

TTTATGAACGTCGCCATGTTGCGTGCGGCACGTTATTTATGGAGCGAAGCGGTCAGTGGATTTGGCGCACAGGACCCGAAATCACTGGCGCTGCGTACCCACTGCCAGACCTCAGGCTGGAGCCTGACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACA  >  NC_000913/3061699‑3061972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`