BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,964	G→T	L241F (TTG→TTT)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,964	0	G	T	100.0%	505.3 / NA	139	L241F (TTG→TTT)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (76/63); total (76/63)

Only 100 of 139 total aligned reads displayed.

CGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTA  >  NC_000913/1290829‑1291099

cGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGgcg                                                                                                                                     <  1:1841657/140‑1 (MQ=255)

cGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGgcg                                                                                                                                     <  2:1295554/140‑1 (MQ=255)

cGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGgcg                                                                                                                                     <  1:278643/140‑1 (MQ=255)

cGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGgcg                                                                                                                                     <  2:1539181/140‑1 (MQ=255)

 gATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGgcgc                                                                                                                                    <  2:1787369/140‑1 (MQ=255)

         aTTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGa                                                                                                                            <  1:248191/140‑1 (MQ=255)

         aTTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGa                                                                                                                            <  1:1670670/140‑1 (MQ=255)

          ttttATTGCCTTTATGTCACCCGAGCTGGACATAATGGCGTTCTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGaa                                                                                                                           <  2:548480/140‑1 (MQ=255)

          ttttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGaa                                                                                                                           <  1:876540/140‑1 (MQ=255)

          ttttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGaa                                                                                                                           <  2:1661212/140‑1 (MQ=255)

          ttttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGaa                                                                                                                           <  2:1462909/140‑1 (MQ=255)

           tttATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAg                                                                                                                          <  2:576597/140‑1 (MQ=255)

             tATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGgcgc                                                                                                                                    <  2:1890807/128‑1 (MQ=255)

             tATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGgcgc                                                                                                                                    >  1:1890807/1‑128 (MQ=255)

             tATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCa                                                                                                                        >  2:1019003/1‑140 (MQ=255)

                tGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGt                                                                                                                     <  1:1783071/140‑1 (MQ=255)

                tGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGt                                                                                                                     <  1:1019003/140‑1 (MQ=255)

                 gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTa                                                                                                                    <  1:1685261/140‑1 (MQ=255)

                 gCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTa                                                                                                                    <  1:401571/140‑1 (MQ=255)

                  ccTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGaa                                                                                                                           <  1:1594250/132‑1 (MQ=255)

                   cTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGt                                                                                                                                  >  2:1628300/1‑124 (MQ=255)

                    ttGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAAc                                                                                                                 >  2:1705714/1‑140 (MQ=255)

                     tGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAAcc                                                                                                                >  2:777728/1‑140 (MQ=255)

                      gATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCa                                                                                                               >  1:854138/1‑140 (MQ=255)

                      gATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCa                                                                                                               <  1:112793/140‑1 (MQ=255)

                            aCCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTaaa                                                                                                                  >  2:373709/1‑131 (MQ=255)

                            aCCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACt                                                                                                         >  1:1595279/1‑140 (MQ=255)

                            aCCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACt                                                                                                         >  1:1191843/1‑140 (MQ=255)

                             cccGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACtt                                                                                                        >  1:1704620/1‑140 (MQ=255)

                                      gACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc                                                                                               >  2:358351/1‑140 (MQ=255)

                                      gACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc                                                                                               >  2:421897/1‑140 (MQ=255)

                                           aaTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCg                                                                                                      >  1:934186/1‑128 (MQ=255)

                                            aTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTg                                                                                         >  2:1835309/1‑140 (MQ=255)

                                             tGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGc                                                                                        <  2:2003344/140‑1 (MQ=255)

                                                cGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgg                                                                                     >  1:1618340/1‑140 (MQ=255)

                                                cGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgg                                                                                     >  2:1578668/1‑140 (MQ=255)

                                                cGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgg                                                                                     >  2:879087/1‑140 (MQ=255)

                                                 gTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCggg                                                                                    <  1:879087/140‑1 (MQ=255)

                                                 gTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACC‑AAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgggg                                                                                   <  2:69934/140‑1 (MQ=255)

                                                  tACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCgggg                                                                                   >  2:966422/1‑140 (MQ=255)

                                                        ctgcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGttt                                                                             <  1:1162205/140‑1 (MQ=255)

                                                            cTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGc                                                                         >  2:812426/1‑140 (MQ=255)

                                                                   ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGt                                                                  <  1:1638210/140‑1 (MQ=255)

                                                                   ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGt                                                                  <  2:76784/140‑1 (MQ=255)

                                                                   ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGt                                                                  <  1:324447/140‑1 (MQ=255)

                                                                   ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGt                                                                  <  2:1036600/140‑1 (MQ=255)

                                                                   ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGt                                                                  <  2:1241417/140‑1 (MQ=255)

                                                                       gcgcATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGc                                                              <  1:84058/140‑1 (MQ=255)

                                                                        cgcATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCTGGGCAGTTTCCGCTATTAGTTGGCt                                                             >  2:452191/1‑140 (MQ=255)

                                                                        cgcATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCt                                                             >  2:1239647/1‑140 (MQ=255)

                                                                          cATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCtat                                                           >  1:709985/1‑140 (MQ=255)

                                                                           aTTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTc                                                                            >  1:1893105/1‑122 (MQ=255)

                                                                           aTTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTc                                                                            <  2:1893105/122‑1 (MQ=255)

                                                                           aTTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCtatt                                                          >  1:1069808/1‑140 (MQ=255)

                                                                           aTTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCtatt                                                          >  1:704208/1‑140 (MQ=255)

                                                                                     ggATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGa                                                <  1:1239647/140‑1 (MQ=255)

                                                                                       attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                              >  2:1194353/1‑140 (MQ=255)

                                                                                       attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                              >  2:494395/1‑140 (MQ=255)

                                                                                       attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                              >  2:591661/1‑140 (MQ=255)

                                                                                       attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                              >  2:2053089/1‑140 (MQ=255)

                                                                                       attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                              >  2:67476/1‑140 (MQ=255)

                                                                                       attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                              >  1:1095770/1‑140 (MQ=255)

                                                                                       attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                              >  2:1712382/1‑140 (MQ=255)

                                                                                         tattaCAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTg                                            >  2:1633626/1‑140 (MQ=255)

                                                                                         tattaCAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTg                                            >  1:1774885/1‑140 (MQ=255)

                                                                                               aGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAt                                      <  2:868751/140‑1 (MQ=255)

                                                                                                 gAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAtct                                    >  2:1900412/1‑140 (MQ=255)

                                                                                                 gAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAtct                                    >  1:1431114/1‑140 (MQ=255)

                                                                                                   aCAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa                                  >  2:1538948/1‑140 (MQ=255)

                                                                                                    cAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAt                                 <  2:1281639/140‑1 (MQ=255)

                                                                                                     aGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAtt                                >  1:1363235/1‑140 (MQ=255)

                                                                                                     aGCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAtt                                >  2:1302230/1‑140 (MQ=255)

                                                                                                      gCTTGCACACCAAAATCAATGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTc                               >  2:1012669/1‑140 (MQ=255)

                                                                                                      gCTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTc                               >  2:40717/1‑140 (MQ=255)

                                                                                                       cTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                              <  1:966422/140‑1 (MQ=255)

                                                                                                       cTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                              >  1:377497/1‑140 (MQ=255)

                                                                                                       cTTGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                              <  1:1312505/140‑1 (MQ=255)

                                                                                                         tGCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTgg                            >  2:733933/1‑140 (MQ=255)

                                                                                                          gCACACCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGt                           <  1:1851653/140‑1 (MQ=255)

                                                                                                           cacaCCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGtt                          >  2:407314/1‑140 (MQ=255)

                                                                                                           cacaCCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGtt                          >  1:281248/1‑140 (MQ=255)

                                                                                                            acacCAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt                         >  1:877564/1‑140 (MQ=255)

                                                                                                               ccAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTg                      <  1:1232492/140‑1 (MQ=255)

                                                                                                               ccAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTg                      <  2:877564/140‑1 (MQ=255)

                                                                                                                cAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGc                     >  1:1364531/1‑140 (MQ=255)

                                                                                                                cAAAATCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCC‑GGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCg                    >  1:1790394/1‑140 (MQ=255)

                                                                                                                 aaatTCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCg                    >  2:953489/1‑140 (MQ=255)

                                                                                                                   aaTCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCggg                  >  2:1372658/1‑140 (MQ=255)

                                                                                                                    aTCAACGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGt                 <  1:732460/140‑1 (MQ=255)

                                                                                                                         cGGTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGaa            <  1:532565/140‑1 (MQ=255)

                                                                                                                           gTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg          <  2:1191843/140‑1 (MQ=255)

                                                                                                                           gTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg          <  1:523625/140‑1 (MQ=255)

                                                                                                                           gTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg          <  1:1633626/140‑1 (MQ=255)

                                                                                                                           gTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg          <  1:2053089/140‑1 (MQ=255)

                                                                                                                           gTTGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg          <  1:1924706/140‑1 (MQ=255)

                                                                                                                            ttGCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGc         >  1:1451340/1‑140 (MQ=255)

                                                                                                                              gCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCg        >  2:1467494/1‑139 (MQ=255)

                                                                                                                              gCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGa       <  2:709985/140‑1 (MQ=255)

                                                                                                                              gCCAGAGTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGa       >  2:342556/1‑140 (MQ=255)

                                                                                                                                  gagTTTGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGtt   <  1:67476/140‑1 (MQ=255)

CGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTA  >  NC_000913/1290829‑1291099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`