Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	22.2%	114.5 / 16.0	54	intergenic (‑179/‑28)	ttdR/ttdA	DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (3/39);  new base T (12/0);  total (15/39)
Fisher's exact test for biased strand distribution p-value = 1.33e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574                                                                                                                                            |                                                                                                                                             aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCa                                                                                                                                            >  1:836998/1‑140 (MQ=255)   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                          <  2:594557/140‑1 (MQ=255)   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                          <  1:1597700/140‑1 (MQ=255)   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                          >  1:443221/1‑140 (MQ=255)   aaCATTGCCATGCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                          >  1:168876/1‑140 (MQ=255)       ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACaaa                                                                                                                                      <  2:264872/140‑1 (MQ=255)          ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                                                   <  1:1223864/140‑1 (MQ=255)          ccATGCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAc                                                                                                                                   <  2:168876/140‑1 (MQ=255)                ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAAc                                                                                                                             <  2:1010533/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                        <  2:706926/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                        <  1:612837/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                        <  1:1832401/140‑1 (MQ=255)                       tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCACAAAAAAAAAAAAAATGGAggt                                                                                                                      >  2:1119850/1‑138 (MQ=255)                        ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGC‑AAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAAAAAAAAAAAAAAAAGGAggttg                                                                                                                    >  2:965929/1‑136 (MQ=255)                         gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAACAAAAAATGGAG‑GTgg                                                                                                                   >  2:1225271/1‑139 (MQ=255)                         gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAG‑TTgg                                                                                                                   >  2:39144/1‑139 (MQ=255)                             aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCatg                                                                                                               <  1:355427/140‑1 (MQ=255)                             aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCatg                                                                                                               <  1:1790720/140‑1 (MQ=255)                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAAAGGAG‑TTGCCatga                                                                                                              >  1:302404/1‑140 (MQ=255)                                   cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAAAGGAG‑TTGCCATGATGAGc                                                                                                         >  1:1683508/1‑140 (MQ=255)                                   cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAG‑TTGCCATGATGAgg                                                                                                         >  1:222619/1‑139 (MQ=255)                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGa                                                                                                       <  1:874911/140‑1 (MQ=255)                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGa                                                                                                       <  2:513163/140‑1 (MQ=255)                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGa                                                                                                       <  2:572030/140‑1 (MQ=255)                                      tcTTATTTTCGCGTTTATTTACGAACTGTTAAATTCCTATTCCCGGTGGCAAAAAATATAAAACCCCAATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGa                                                                                                       <  2:416714/140‑1 (MQ=255)                                        ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAg                                                                                                    <  1:1909904/140‑1 (MQ=255)                                          aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGta                                                                                                  <  2:1975960/140‑1 (MQ=255)                                           ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAAACAAAAAAAAAAAAAGGAG‑GTTCCATGATGAGCGaaaaaaa                                                                                                 >  2:629872/1‑136 (MQ=255)                                             ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGtaata                                                                                               <  1:285268/140‑1 (MQ=255)                                                      aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                      <  2:976140/140‑1 (MQ=255)                                                      aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                      <  2:1143474/140‑1 (MQ=255)                                                       ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                     <  1:1225271/140‑1 (MQ=255)                                                         cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg                                                                                   <  2:443221/140‑1 (MQ=255)                                                           cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCA‑ACAAAAACAAAAAATGGAG‑TTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTg                                                                                 >  2:271193/1‑140 (MQ=255)                                                             aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                               <  1:1185671/140‑1 (MQ=255)                                                             aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                               <  1:341394/140‑1 (MQ=255)                                                             aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                               <  2:836100/140‑1 (MQ=255)                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                            <  1:336676/140‑1 (MQ=255)                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                            <  1:1828645/140‑1 (MQ=255)                                                                  ttAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAACAAAAAATGGAG‑TTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGGGAATaaat                                                                          >  1:1039662/1‑138 (MQ=255)                                                                    aaTTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAAAAAAAAATGGAG‑TTGCCATGATGAGCGGAAGGAATAAAAAAAAAGCAGAGAATAAATTg                                                                        >  2:1366063/1‑140 (MQ=255)                                                                            atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAATAAAAAAAATGGAG‑GTGGCATGATGAGGGAAAGTAATAAGCAACAAGCAGTGAATAAGTTGGCAGAGAt                                                                >  2:1809659/1‑140 (MQ=255)                                                                            atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAATGGAG‑GTGCCATGATGAGCGAAAATAATAAAAAACAAGCAGTGAATAAAATGACAgagaa                                                                >  2:1251828/1‑139 (MQ=255)                                                                            atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAAAAAAAAAAAAAAAAGGGG‑TTGGCATGATGAGAGAAAGTAAAAAAAAAAAAGCAGAGAATAAAGTTACAgagaa                                                                >  2:1992801/1‑139 (MQ=255)                                                                             ttCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                               <  1:688123/140‑1 (MQ=255)                                                                                aCGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGGG‑AAATTTTTCTGACAAAAACAAAAAATGGAGATGGCCAT‑ATGGGCGAAAGAAATAAAAAAAAAACAGGGAATAAGGTGAAAgagagtttt                                                            >  2:1956892/1‑135 (MQ=255)                                                                                   gTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAAAGGAG‑GTGGCATGATGAGGGAAAGGAAAAAACAAAAAGCAATGAATAAATTGACAGAGAATGttggc                                                         >  2:2030578/1‑136 (MQ=255)                                                                                    tGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAG‑GTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAATAAATTGACAGAGATTGTCGCTa                                                        >  2:878505/1‑140 (MQ=255)                                                                                      gCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAATAAAAAAAATGGAG‑GTGCCGTGGTGGGGGGAAGTAAAAAACAAAAAGCAGTGAATAAATTGGAAGAGATTGTCGCTAAc                                                      >  2:874264/1‑140 (MQ=255)                                                                                              tataAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAAAGGAG‑GTGCCCTGATGAGGGAAAGTAAAAAACAAAAAGCAGGGAAAAAATTTACAGAGAATTTCGCTAAAttttaccc                                              >  2:1506254/1‑135 (MQ=255)                                                                                                taAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                            <  2:763996/140‑1 (MQ=255)                                                                                                aaaaaCCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                            <  2:243374/139‑1 (MQ=255)                                                                                                aaaaaCCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                            <  2:1427797/139‑1 (MQ=255)                                                                                                      cac‑aTTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAACAAAAAATGGAG‑GTGCCATGATGAGCGAAAGTAAAAAAAAAAAAGCAGTGAAAAAGTTGACAGAGATTGTCGCTAAATTTACCCCCCTGAttt                                      >  2:1636081/1‑140 (MQ=255)                                                                                                       gc‑aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTc                                     <  1:914714/139‑1 (MQ=255)                                                                                                               tAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                              <  2:1683508/140‑1 (MQ=255)                                                                                                                    gTAGTTTGTGGCGGTG‑GATTTTTTCCGACAAAAACAAAAAATGGAG‑GTGCCCTGATGAGCGAAAGTAATAAACAAAAGGCAGGGAAAAAATTGACAGAGATTGTCGCTAAATTTTCCGCCCTGATTTTTACCCGAATGcc                         >  2:914714/1‑140 (MQ=255)                                                                                                                    gTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc                         <  2:836998/140‑1 (MQ=255)                                                                                                                      aGTTTGTGGCGGTG‑AATTTTTTCCGAAAAAAAAAAAAAATGGAG‑GTGGCATGATGGGGGAAAAAAATAAACAAAAGGCAATGAATAAGATGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTTCCCCAATGCCtg                       >  2:809682/1‑140 (MQ=255)                                                                                                                        tttGTGGGGGTA‑AATTTTTCCAGACAAATACAAAAACGGGAG‑TTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCtgat                     <  2:1129274/140‑1 (MQ=255)                                                                                                                         ttGTGGCGGTG‑AATTTTTCAAGACAAATAAAAAAACGGGAG‑TTGCAATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATATGTGGAGAGAATTTGCCGAAAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                    <  2:996823/140‑1 (MQ=255)                                                                                                                          tgtgGCGGTG‑AATTTTTTCAGACAAATACAAAAAAAGGAG‑TTGGCATGATGGGGGAAAGAAATAAACAACAGGCAGTGAATAAAGTGACAGAGATTGTCGCTAACTTTACCCCCATGATTTTTACCCGCATGCCtgatga                   >  2:623919/1‑140 (MQ=255)                                                                                                                            tgGCGGTG‑GATTTTTCCCGACAAATACAAAAACTGGAG‑GTGCCATGATGAGAGCAAGAAATAAGAAAAAGGCAGGGAAAAAGTTGACAGAGATTGTCGCTAACTTTTCCCCCATGATTTTTAACCGAATGCCTGATGACg                 >  2:644635/1‑140 (MQ=255)                                                                                                                             ggCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                <  1:1199382/140‑1 (MQ=255)                                                                                                                             ggCGGTG‑AATTTTTCCAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                <  1:2030578/140‑1 (MQ=255)                                                                                                                             ggCGGTG‑AATTTTTCAAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                <  2:1351001/140‑1 (MQ=255)                                                                                                                              gCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGGG‑GTGCCCTGATGTGCGAAAAGAATAAAAAAAAAGAAGTGAATAAATTGACAGAGATTGTCGCTAAATTTTCCCCCCTGATTTCTACCCCAATGCCCGATGACggg               >  2:560004/1‑138 (MQ=255)                                                                                                                              gCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg               <  1:1119850/140‑1 (MQ=255)                                                                                                                                 gTG‑GATTTTTTCAGACAAATACAAAAAATGGAG‑GTTGCATGGTGAGCGGAAGTAATAAACAACAAGCAAGGAAAAAAGTGAAAGAGATTTTCGCTAACTTTACCGCCATGATTTTTACCCCAAAGCCTGATTACgggggg            >  2:781633/1‑138 (MQ=255)                                                                                                                                 gTG‑AATTTTTTCAGACAAATACAAAAAATGGAG‑TTGCCATGATGAGGGAAAGGAATAAGCAACAAGCAGTGAAAAAGATGACAGAGATTGTCGCTAACTTTACCCCCATGATTTTTACCCGAATGCCTGATGAAgtggtg            >  2:254045/1‑140 (MQ=255)                                                                                                                                   g‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  2:222619/140‑1 (MQ=255)                                                                                                                                   g‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:1477501/140‑1 (MQ=255)                                                                                                                                    aattttttCCAGACAAATACAAAAACTGGAG‑TTGCAATGATGAGCGAAAGTAATAAGCAACAGGCATTAAATAAGTTGACAGAGATTGTCTCAACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  2:938020/137‑1 (MQ=255)                                                                                                                                            ccAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  1:436804/140‑1 (MQ=255)                                                                                                                                            ccAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  1:1731652/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑