Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	212,802	C→A	R158S (CGC→AGC)	tilS →	tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	212,802	0	C	A	100.0%	291.7 / NA	81	R158S (CGC→AGC)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/57);  total (24/57)

ACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGCCGTTATTGCAGC  >  NC_000913/212667‑212928                                                                                                                                        |                                                                                                                               aCCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCAc                                                                                                                            >  1:528356/1‑140 (MQ=255) aCCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCAc                                                                                                                            <  2:476326/140‑1 (MQ=255) aCCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCAc                                                                                                                            >  2:1428368/1‑140 (MQ=255)  ccGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCAcg                                                                                                                           <  2:518228/140‑1 (MQ=255)   cgcgCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCAcgc                                                                                                                          >  2:493515/1‑140 (MQ=255)     cgcAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCgg                                                                                                                        >  2:579274/1‑140 (MQ=255)         aCATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCgggggg                                                                                                                    <  1:843087/140‑1 (MQ=255)           aTCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGaa                                                                                                                  <  1:1011440/140‑1 (MQ=255)               cgacgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTgg                                                                                                              >  2:840466/1‑140 (MQ=255)                 acgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTg                                                                                                            <  1:1653871/140‑1 (MQ=255)                    aTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAg                                                                                                         >  2:791287/1‑140 (MQ=255)                    aTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAg                                                                                                         >  1:1553086/1‑140 (MQ=255)                        aTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCa                                                                                                          >  2:1788409/1‑135 (MQ=255)                        aTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCa                                                                                                          <  1:1788409/135‑1 (MQ=255)                        aTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTggg                                                                                                     <  1:1750473/140‑1 (MQ=255)                        aTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTggg                                                                                                     <  1:752314/140‑1 (MQ=255)                        aTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTggg                                                                                                     <  2:1936564/140‑1 (MQ=255)                          gtgAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGgcg                                                                                                   <  1:186106/140‑1 (MQ=255)                             aaaCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGt                                                                                                <  1:486142/140‑1 (MQ=255)                               aCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCa                                                                                              >  2:1316588/1‑140 (MQ=255)                                ccTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAg                                                                                             <  2:1831864/140‑1 (MQ=255)                                 cTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGt                                                                                            <  1:306694/140‑1 (MQ=255)                                  tttCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTa                                                                                           <  1:292503/140‑1 (MQ=255)                                   ttCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTg                                                                                                            >  2:485729/1‑122 (MQ=255)                                   ttCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTg                                                                                                            <  1:485729/122‑1 (MQ=255)                                   ttCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTAt                                                                                          >  1:740000/1‑140 (MQ=255)                                      tgctgGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGAt                                                                                       <  1:1249389/140‑1 (MQ=255)                                        ctgGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGAttt                                                                                     >  1:85051/1‑140 (MQ=255)                                        ctgGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGAttt                                                                                     >  1:1194303/1‑140 (MQ=255)                                          ggCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTAc                                                                                   >  2:1957104/1‑140 (MQ=255)                                           gcgcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACg                                                                                  <  1:1348173/140‑1 (MQ=255)                                           gcgcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACg                                                                                  <  1:3212/140‑1 (MQ=255)                                             gcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCt                                                                                >  1:1768184/1‑140 (MQ=255)                                                    cgcgGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGa                                                                         >  2:1194586/1‑140 (MQ=255)                                                       ggCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGa                                                                      <  1:1194586/140‑1 (MQ=255)                                                       ggCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGa                                                                      <  2:1553086/140‑1 (MQ=255)                                                       ggCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGa                                                                      <  2:155902/140‑1 (MQ=255)                                                          aGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGa                                                                   <  1:1428368/140‑1 (MQ=255)                                                            tGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGaaa                                                                 <  2:1887598/140‑1 (MQ=255)                                                              gCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGt                                                               >  2:1159656/1‑140 (MQ=255)                                                                 cTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAAt                                                            <  1:1684993/140‑1 (MQ=255)                                                                    ccGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAg                                                         <  1:835385/140‑1 (MQ=255)                                                                     cGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAgg                                                        <  1:402795/140‑1 (MQ=255)                                                                      gggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGga                                                       <  2:1866835/140‑1 (MQ=255)                                                                      gggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGga                                                       <  2:528356/140‑1 (MQ=255)                                                                      gggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGga                                                       <  2:1050975/140‑1 (MQ=255)                                                                      gggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGga                                                       <  2:220729/140‑1 (MQ=255)                                                                      gggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGga                                                       <  2:1708867/140‑1 (MQ=255)                                                                      gggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGga                                                       <  2:645464/140‑1 (MQ=255)                                                                      gggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGga                                                       <  2:851289/140‑1 (MQ=255)                                                                       ggCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgac                                                      <  2:1937581/140‑1 (MQ=255)                                                                        gCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacg                                                     <  2:85051/140‑1 (MQ=255)                                                                        gCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacg                                                     <  1:1993534/140‑1 (MQ=255)                                                                        gCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGAAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacg                                                     >  2:370464/1‑140 (MQ=255)                                                                         cTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacga                                                    <  2:1631136/140‑1 (MQ=255)                                                                         cTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacga                                                    <  1:1518659/140‑1 (MQ=255)                                                                          tttCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacgac                                                   <  1:1231872/140‑1 (MQ=255)                                                                            tCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTc                                                 >  2:1428457/1‑140 (MQ=255)                                                                                cTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATAc                                             <  2:1768184/140‑1 (MQ=255)                                                                                  aTGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCAt                                               <  2:776937/136‑1 (MQ=255)                                                                                  aTGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCAt                                               >  1:776937/1‑136 (MQ=255)                                                                                  aTGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGa                                           <  2:740000/140‑1 (MQ=255)                                                                                      cGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGt                                       <  1:579274/140‑1 (MQ=255)                                                                                      cGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGt                                       >  1:1505901/1‑140 (MQ=255)                                                                                          aGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATAc                                             <  2:891169/130‑1 (MQ=255)                                                                                          aGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATAc                                             >  1:891169/1‑130 (MQ=255)                                                                                              tcGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTct                               <  2:1664466/140‑1 (MQ=255)                                                                                               cGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGTAAGTAATCAGGACGACTCATACGATCGTAACTTTctg                              >  1:299846/1‑140 (MQ=255)                                                                                               cGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTctg                              <  2:1916701/140‑1 (MQ=255)                                                                                                      tGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTctgcgcctgc                       <  2:372275/140‑1 (MQ=255)                                                                                                      tGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTctgcgcctgc                       >  1:1365710/1‑140 (MQ=255)                                                                                                        ccGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTctgcgcctgcgc                     <  1:843656/140‑1 (MQ=255)                                                                                                         cGGAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGcgcg                    <  2:1344149/140‑1 (MQ=255)                                                                                                          ggAACGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGt                   <  1:2033356/140‑1 (MQ=255)                                                                                                            aaCGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAg                 <  1:493515/140‑1 (MQ=255)                                                                                                             aCGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGt                <  1:791287/140‑1 (MQ=255)                                                                                                             aCGCGGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGt                <  1:2016296/140‑1 (MQ=255)                                                                                                                cgGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGcc             <  1:644762/140‑1 (MQ=255)                                                                                                                cgGCTTATTCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGcc             <  1:1203392/140‑1 (MQ=255)                                                                                                                       ttCGCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGCCGTTATTg      <  2:1365710/140‑1 (MQ=255)                                                                                                                          gCCCGTTGCTCGCCAGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGCCGTTATTgcag   <  1:1375868/140‑1 (MQ=255)                                                                                                                           cccGTTGCTCGCCAGCACGCGGGGGGAACTGGTGAAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGCCGTTATTgcagc  <  2:1493620/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                               ACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGCCGTTATTGCAGC  >  NC_000913/212667‑212928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑